scholarly journals Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 533 ◽  
Author(s):  
Jiao-jiao Ji ◽  
Qi Feng ◽  
Hai-feng Sun ◽  
Xue-jun Zhang ◽  
Xiao-xiao Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Multiple Shoots ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Codonopsis Pilosula ◽  
Meja Treatment ◽  
Metabolite Accumulation ◽  
Culture Growth ◽  
Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

scholarly journals A Review of Antiviral and Antioxidant Activity of Bioactive Metabolite of Macroalgae within an Optimized Extraction Method

Energies ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3092
Author(s):  
Rasha El-Shafei ◽  
Hala Hegazy ◽  
Bishnu Acharya
Keyword(s):  
Antioxidant Activity ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Extraction Method ◽  
Bioactive Metabolites ◽  
Active Metabolites ◽  
Bioactive Metabolite ◽  
Conventional Extraction ◽  
Simplex Virus ◽  
Macro Algae

Non-conventional extraction of bioactive metabolites could provide sustainable alternative techniques to preserve the potency of antioxidants and antiviral compounds extracted from macro-algae. In this paper, we first reviewed the antioxidant and antiviral potential of the active metabolites that exist in the three known macro-algae classes; Phaeophyceae, Rhodophyceae, and Chlorophyceae, and a comparison between their activities is discussed. Secondly, a review of conventional and non-conventional extraction methods is undertaken. The review then focused on identifying the optimal extraction method of sulphated polysaccharide from macro-algae that exhibits both antiviral and antioxidant activity. The review finds that species belonging to the Phaeophyceae and Rhodophceae classes are primarily potent against herpes simplex virus, followed by human immunodeficiency virus and influenza virus. At the same time, species belonging to Chlorophyceae class are recorded by most of the scholars to have antiviral activity against herpes simplex virus 1. Additionally, all three macro-algae classes exhibit antioxidant activity, the potency of which is a factor of the molecular structure of the bioactive metabolite as well as the extraction method applied.

Optimization of cultural conditions affecting improved bioactive metabolite production by endophytic fungus Trichoderma harzianum

Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2187-2192
Author(s):  
Rashid Rahim Hateet ◽  
Zainab Alag Hassan ◽  
Abdulameer Abdullah Al-Mussawi ◽  
Shaima Rabeea Banoon
Keyword(s):  
Antibacterial Activity ◽  
Trichoderma Harzianum ◽  
Endophytic Fungus ◽  
Nitrogen Sources ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Metabolite Production ◽  
Surface Sterilization ◽  
Cultural Conditions

The present study aimed to optimize cultural conditions for optimum bioactive metabolite production by endophytic fungus Trichoderma harzianum, isolated by surface sterilization method from the leaf of the eucalyptus plant. The fungus was identified based on morphological characterization. Fungal metabolites were carried out by ethyl acetate solvent. The antibacterial activity was tested against Escherichia coli (ATCC 25922) and Staphylococcus aureus (NCTC 6571). Various carbon, nitrogen sources, pH, temperature, incubation period, and NaCl on the antibacterial metabolite production were studied. Bioactive metabolite production of T. harzianum exhibits a broad spectrum of in vitro antibacterial activity against two strains of bacteria. For the optimum production of bioactive metabolites, Dextrose and Glucose were found to be the best sources of carbon and the best sources of Nitrogen Yeast extract (YE) and (NH4)2SO. The maximum production of bioactive metabolites occurs at pH 7 and 25°C.; the NaCl showed a positive influence on bioactive metabolites.

Environmental Factors Impacting Bioactive Metabolite Accumulation in Brazilian Medicinal Plants

2019 ◽  
pp. 109-134
Author(s):  
Camila Fernanda de Oliveira Junkes ◽  
Franciele Antonia Neis ◽  
Fernanda de Costa ◽  
Anna Carolina Alves Yendo ◽  
Arthur Germano Fett-Neto
Keyword(s):  
Medicinal Plants ◽  
Environmental Factors ◽  
Bioactive Metabolite ◽  
Metabolite Accumulation ◽  
Brazilian Medicinal Plants

The effects of methyl jasmonate on growth, gene expression and metabolite accumulation in Isatis indigotica Fort.

2022 ◽  
Vol 177 ◽  
pp. 114482
Author(s):  
Rui Liu ◽  
Zirong Wang ◽  
Jinyu Zheng ◽  
Zhaojin Xu ◽  
Xue Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methyl Jasmonate ◽  
Isatis Indigotica ◽  
Metabolite Accumulation

scholarly journals Effect of Methyl Jasmonate on Phenolic Accumulation in Wounded Broccoli

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3537 ◽  
Author(s):  
Yuge Guan ◽  
Wenzhong Hu ◽  
Aili Jiang ◽  
Yongping Xu ◽  
Rengaowa Sa ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Enzyme Activities ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phenylpropanoid Metabolism ◽  
Antioxidant Power ◽  
Meja Treatment ◽  
Coenzyme A Ligase ◽  
Key Enzyme

In order to find an efficient way for broccoli to increase the phenolic content, this study intended primarily to elucidate the effect of methyl jasmonate (MeJA) treatment on the phenolic accumulation in broccoli. The optimum concentration of MeJA was studied first, and 10 μM MeJA was chosen as the most effective concentration to improve the phenolic content in wounded broccoli. Furthermore, in order to elucidate the effect of methyl jasmonate (MeJA) treatment on phenolic biosynthesis in broccoli, the key enzyme activities of phenylpropanoid metabolism, the total phenolic content (TPC), individual phenolic compounds (PC), antioxidant activity (AOX) and antioxidant metabolism-associated enzyme activities were investigated. Results show that MeJA treatment stimulated phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarin coenzyme A ligase (4CL) enzymes activities in phenylpropanoid metabolism, and inhibited the activity of polyphenol oxidase (PPO), and further accelerated the accumulation of the wound-induced rutin, caffeic acid, and cinnamic acid accumulation, which contributed to the result of the total phenolic content increasing by 34.8% and ferric reducing antioxidant power increasing by 154.9% in broccoli. These results demonstrate that MeJA in combination with wounding stress can induce phenylpropanoid metabolism for the wound-induced phenolic accumulation in broccoli.

scholarly journals Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 447 ◽  
Author(s):  
Felipe Valenzuela-Riffo ◽  
Paz E. Zúñiga ◽  
Luis Morales-Quintana ◽  
Mauricio Lolas ◽  
Marcela Cáceres ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Botrytis Cinerea ◽  
Defense Responses ◽  
Gray Mold ◽  
The Past ◽  
Defense Systems ◽  
Meja Treatment ◽  
Fruit Development And Ripening ◽  
Genes Encoding ◽  
And Control

Several attempts have been made to study the effects of methyl jasmonate (MeJA) on plants in the past years. However, the comparative effects of the number and phenological time of MeJA applications on the activation of defense systems is currently unknown in strawberries. In the present research, we performed three field treatments during strawberry (Fragaria × ananassa ‘Camarosa’) fruit development and ripening which consisted of differential MeJA applications at flowering (M3), and the large green (M2 and M3) and red ripe (M1, M2, and M3) fruit stages. We also checked changes in gene expression related to plant defense against Botrytis cinerea inoculation post-harvest. In M3 treatment, we observed an upregulation of the anthocyanin and lignin contents and the defense-related genes, encoding for chitinases, β-1,3-glucanases and polygalacturonase-inhibiting proteins, after harvest (0 hpi), along with the jasmonate signaling-related genes FaMYC2 and FaJAZ1 at 48 h after B. cinerea inoculation (48 hpi) during postharvest storage. Although we did not find differences in gray mold incidence between the MeJA treatments and control, these results suggest that preharvest MeJA treatment from the flowering stage onwards (M3) primes defense responses mediated by the upregulation of different defense-related genes and retains the upregulation of MYC2 and JAZ1 at 48 hpi.

Physiological trade-offs associated with methyl jasmonate - induced resistance in Pinus radiata

2008 ◽  
Vol 38 (4) ◽  
pp. 677-684 ◽  
Author(s):  
Nick Gould ◽  
Tony Reglinski ◽  
Mike Spiers ◽  
Joe T. Taylor
Keyword(s):  
Growth Rate ◽  
Disease Resistance ◽  
Methyl Jasmonate ◽  
Water Potential ◽  
Seedling Growth ◽  
Pinus Radiata ◽  
Transpiration Rate ◽  
Meja Treatment ◽  
Seedling Growth Rate ◽  
The Impact

Methyl jasmonate (MeJA) can induce defence responses in plants to pathogen attack, but it can also have consequences for plant growth. The transient effects of exogenous MeJA treatment on the resistance of Monterey pine ( Pinus radiata D. Don) seedlings to Diplodia pinea (Desm.) Kickx. and some physiological parameters affecting the impact of treatment on seedling growth were investigated. Following foliar application of 4.5 mmol·L–1 MeJA, disease resistance was greatest 1–2 weeks after treatment and declined with time thereafter. Elevated disease resistance was accompanied by a reduction in seedling growth rate the second week following MeJA treatment. Thereafter, seedling growth rate recovered and exceeded that of the control seedlings 4–5 weeks after MeJA treatment. Within hours of MeJA treatment, reductions in both the capacity of photosystem II and transpiration rate were observed, resulting in a concomitant reduction in net CO2 uptake rate. The slight reduction in transpiration rate was also associated with an increase in needle water potential. Longer term measurements showed no effect of MeJA on photosynthetic rate, transpiration rate, chlorophyll content, or shoot water potential and thus could not account for the elevated growth rate observed 4–5 weeks after treatment.

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