Hexane partition from Annona crassiflora Mart. promotes cytotoxity and apoptosis on human cervical cancer cell lines

2018 ◽  
Vol 37 (4) ◽  
pp. 602-615 ◽  
Author(s):  
Viviane A. O. Silva ◽  
Ana Laura V. Alves ◽  
Marcela N. Rosa ◽  
Larissa R. V. Silva ◽  
Matias E. Melendez ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Cervical Cancer Cell ◽  
Human Cervical Cancer Cell ◽  
Annona Crassiflora ◽  
Human Cervical Cancer

Su.71. MHC Class-I Related Chain B and Not Chain A is Preferentially Expressed on Human Cervical Cancer Cell Lines

2008 ◽  
Vol 127 ◽  
pp. S147
Author(s):  
Luis Jave-Suarez ◽  
Naela Arreygue-Garcia ◽  
Adriana Aguilar-Lemarrroy ◽  
Miriam Jimenez-Perez ◽  
Angel Arregui ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Mhc Class I ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Class I ◽  
Cervical Cancer Cell ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Ectonucleotidase expression profile and activity in human cervical cancer cell lines

2014 ◽  
Vol 92 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Aline Beckenkamp ◽  
Danielle Bertodo Santana ◽  
Alessandra Nejar Bruno ◽  
Luciane Noal Calil ◽  
Emerson André Casali ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Adenine Nucleotide ◽  
Expression Profiles ◽  
Cancer Cell Lines ◽  
Cervical Cancer Cell ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Cervical cancer is the third most frequent cancer in women worldwide. Adenine nucleotide signaling is modulated by the ectonucleotidases that act in sequence, forming an enzymatic cascade. Considering the relationship between the purinergic signaling and cancer, we studied the E-NTPDases, ecto-5′-nucleotidase, and E-NPPs in human cervical cancer cell lines and keratinocytes. We evaluated the expression profiles of these enzymes using RT-PCR and quantitative real-time PCR analysis. The activities of these enzymes were examined using ATP, ADP, AMP, and p-nitrophenyl-5′-thymidine monophosphate (p-Nph-5′-TMP) as substrate, in a colorimetric assay. The extracellular adenine nucleotide hydrolysis was estimated by HPLC analysis. The hydrolysis of all substrates exhibited a linear pattern and these activities were cation-dependent. An interesting difference in the degradation rate was observed between cervical cancer cell lines SiHa, HeLa, and C33A and normal imortalized keratinocytes, HaCaT cells. The mRNA of ecto-5′-nucleotidase, E-NTPDases 5 and 6 were detectable in all cell lines, and the dominant gene expressed was the Entpd 5 enzyme, in SiHa cell line (HPV16 positive). In accordance with this result, a higher hydrolysis activity for UDP and GDP nucleotides was observed in the supernatant of the SiHa cells. Both normal and cancer cells presented activity and mRNAs of members of the NPP family. Considering that these enzymes exert an important catalytic activity, controlling purinergic nucleotide concentrations in tumors, the presence of ectonucleotidases in cervical cancer cells can be important to regulate the levels of extracellular adenine nucleotides, limiting their effects.

scholarly journals Phosphatidylinositol 3-Kinase Inhibition by LY294002 Radiosensitizes Human Cervical Cancer Cell Lines

2006 ◽  
Vol 12 (1) ◽  
pp. 250-256 ◽  
Author(s):  
Christopher M. Lee ◽  
Christa B. Fuhrman ◽  
Vicente Planelles ◽  
Morgan R. Peltier ◽  
David K. Gaffney ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Cervical Cancer Cell ◽  
Phosphatidylinositol 3 Kinase ◽  
Kinase Inhibition ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer ◽  
Phosphatidylinositol 3
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