Isolation and characterization of nodules endophytic bacteria Pseudomonas protegens Sneb1997 and Serratia plymuthica Sneb2001 for the biological control of root-knot nematode

2021 ◽  
Vol 164 ◽  
pp. 103924
Author(s):  
Jinjie Zhao ◽  
Shuai Wang ◽  
Xiaofeng Zhu ◽  
Yuanyuan Wang ◽  
Xiaoyu Liu ◽  
...  
Keyword(s):  
Biological Control ◽  
Endophytic Bacteria ◽  
Root Knot Nematode ◽  
Serratia Plymuthica ◽  
Isolation And Characterization

scholarly journals Short Communication: Isolation and characterization of the endophytic bacteria, and their potential as maize diseases control

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Endang Mugiastuti ◽  
SUPRAYOGI ◽  
NUR PRIHATININGSIH ◽  
LOEKAS SOESANTO
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Endophytic Bacteria ◽  
Short Communication ◽  
Plant Protection ◽  
Mechanical Test ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Isolation And Characterization

Abstract. Mugiastuti E, Suprayogi, Prihatiningsih N, Soesanto L. 2020. Short Communication: Isolation And Characterization Of The Endophytic Bacteria, And Their Potential As Maize Diseases Control. Biodiversitas 21: 1809-1815. Sheath blight and bacterial wilt are diseases that can reduce maize production. Biological control with the endophytic bacteria offers environmentally friendly control for these pathogens. The study aimed to isolate and characterize the endophytic bacteria morphologically and biochemically and to study their potential to control maize diseases, especially sheat blight and bacterial wilt causing pathogens. The study was conducted at the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University, from April to August 2019. The study consisted of four stages: isolation and characterization of endophytic bacteria, the antagonism test of the endophytic bacterial to R solani, the antagonism test of the endophytic bacteria to Pantoea sp., and the mechanical test of the endophytic bacteria as biological control agents and plant growth-promoting bacteria. Based on the research, four endophytic bacteria isolates have been successfully isolated, and characterized successfully and found have the potential to be developed as biopesticides to control maize disease, especially R. solani and Pantoea sp. Bacillus sp, endophytic from the root (BK.A1; BK.A3; PP.A5) and Bacillus sp. endophytic from the stem (PPD.B2) can suppress the growth of R.solani by more than 50%, have a strong antagonistic index against Pantoea sp (> 4), and can produce protease and lipase enzyme, and phosphate solubilization.

Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties

2007 ◽  
Vol 44 (1) ◽  
pp. 155-162 ◽  
Author(s):  
Pham Q. Hung ◽  
Senthil M. Kumar ◽  
V. Govindsamy ◽  
K. Annapurna
Keyword(s):  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Cultivated Soybean

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria Bacillus stratosphericus LW-03 from Lilium wardii

3 Biotech ◽  
2020 ◽  
Vol 10 (7) ◽  
Author(s):  
Mohammad Sayyar Khan ◽  
Junlian Gao ◽  
Mingfang Zhang ◽  
Xuqing Chen ◽  
The Su Moe ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

Isolation and Characterization of Endophytic Bacteria from the Nickel Hyperaccumulator Plant Alyssum bertolonii

2007 ◽  
Vol 53 (2) ◽  
pp. 306-316 ◽  
Author(s):  
Rita Barzanti ◽  
Francesca Ozino ◽  
Marco Bazzicalupo ◽  
Roberto Gabbrielli ◽  
Francesca Galardi ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Nickel Hyperaccumulator ◽  
Hyperaccumulator Plant

Isolation and Characterization of Agar-Degrading Endophytic Bacteria from Plants

2014 ◽  
Vol 70 (2) ◽  
pp. 275-281 ◽  
Author(s):  
Tao Song ◽  
Weijia Zhang ◽  
Congchong Wei ◽  
Tengfei Jiang ◽  
Hui Xu ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Isolation And Characterization

scholarly journals Bakteri Endofit asal Tanaman Tembakau sebagai Agens Pengendali Meloidogyne spp.

2019 ◽  
Vol 14 (6) ◽  
pp. 215 ◽  
Author(s):  
Isnainy dinul Mursyalatiyus ◽  
Abdul Munif ◽  
Abdjad Asih Nawangsih
Keyword(s):  
Biological Control ◽  
Endophytic Bacteria ◽  
Biological Control Agent ◽  
Tobacco Plant ◽  
Control Agent ◽  
Root Knot Nematode ◽  
Tobacco Plants ◽  
Physiological Characterization ◽  
Protease Enzyme ◽  
Meloidogyne Spp

Endophytic Bacteria from Tobacco Plant as Biocontrol Agent of  Meloidogyne spp.Soilborne disease on tobacco plants caused by fungal and bacterial infection in association with root-knot nematode (Meloidogyne spp.) may cause significant yield loss.  Endophytic bacteria have been recognized as biological control agent for Meloidogyne spp. as well as  plant promoting growth agent. Research was conducted to evaluate endophytic bacteria isolated from tobacco plants as biological control agent for Meloidogyne spp. infecting tobacco. A total of 215 isolates of endophytic bacteria were isolated from root of two tobacco varieties, Kemloko and Prancak 95.  Biosafety screening showed that 80 isolates (37%) and 7 isolates (8%) gave negative reaction on hypersensitivity test and hemolysis test, respectively.  Seven isolates i.e. TPT3.10, TPT2.1, TK3n8, TK2t21, TK2n8, TK3n1 and TK2t11 were able to promote plant growth and increase the mortality of juvenile Meloidogyne spp. Physiological characterization of endophytic bacteria showed that most of the isolates were able to produce protease enzyme, phosphate, nitrogen and HCN. The same isolates were also able to suppress the number of galls from 80.09% up to 93.82%. Two isolates, TPT3.10 and TK2n8, are considered having the best suppression on root gall formation.

scholarly journals Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer

2015 ◽  
Vol 2 (2) ◽  
pp. 86-98
Author(s):  
Dina Dyah Saputri ◽  
Maria Bintang ◽  
Fachriyan H Pasaribu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Lantana Camara ◽  
Rrna Gene ◽  
Antibacterial Compounds ◽  
Isolation And Characterization ◽  
Ms Analysis

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.

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