Identification and characterization of responsive genes in rice during compatible interactions with pathogenic pathovars of Xanthomonas oryzae

2017 ◽  
Author(s):  
Moein Khojasteh ◽  
Bahman Khahani ◽  
Mohsen Taghavi ◽  
Elahe Tavakol
Keyword(s):  
Xanthomonas Oryzae ◽  
Identification And Characterization ◽  
Compatible Interactions

scholarly journals Identification and characterization of virulence factor of several Indonesian Xanthomonas oryzae pv. oryzae

2014 ◽  
Vol 8 (3) ◽  
pp. 103-111
Author(s):  
FATIMAH FATIMAH ◽  
◽  
APON ZAENAL MUSTOPA ◽  
IQBAL KUSNANDARSYAH
Keyword(s):  
Virulence Factor ◽  
Xanthomonas Oryzae ◽  
Identification And Characterization

Identification and characterization of rice circular RNAs responding to Xanthomonas oryzae pv. oryzae invasion

2021 ◽  
Author(s):  
Peihong Wang ◽  
Sai Wang ◽  
Yan Wu ◽  
Wenhan Nie ◽  
Ayizekeranmu Yiming ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Enrichment Analysis ◽  
Xanthomonas Oryzae ◽  
Pcr Analysis ◽  
Circular Rnas ◽  
Transcriptional Gene Regulation ◽  
Identification And Characterization ◽  
Host Genes

Emerging role of circular RNAs (circRNAs) in various biological processes have advanced our knowledge of transcriptional and post-transcriptional gene regulation. To date, no research has been conducted to explore their roles in the rice- Xanthomonas oryzae pv. oryzae (Xoo) interaction. Therefore, we identified 3517 circRNAs from the highly virulent Xoo strain PXO99A-infected rice leaves using the ribosomal RNA (rRNA) depleted RNA-sequencing technique coupled with the CIRI2 and CIRCexplorer2 pipeline. Characterization analyses showed that these circRNAs were distributed across the whole genome of rice, and most circRNAs arised from exons (85.13 %), ranged from 200 bp to 1000 bp and were with a non-canonical GT/AG (including CT/AC equivalent) splicing signal. Functional annotation and enrichment analysis of the host genes that produced the DEcircRNAs suggested that these identified circRNAs might play an important role in reprogramming rice responses to PXO99A invasion, mainly by mediating photorespiration, chloroplast, peroxisome and diterpenoid biosynthesis. Moreover, 31 differentially expressed circRNAs (DEcircRNAs) were predicted to act as miRNA decoys in rice. The expression profile of 4 DEcircRNAs were validated by RT-qPCR with divergent primers, and the back-splicing sites of seven DEcircRNAs were verified by PCR analysis and Sanger sequencing. Collectively, these results inferred a potential functional role of circRNAs in the regulation of rice immunity and provide novel clues for revealing the molecular mechanisms of rice-PXO99A interaction.

scholarly journals Identification and Characterization of 2′-Deoxyuridine from the Supernatant of Conidial Suspensions of Rice Blast Fungus as an Infection-Promoting Factor in Rice Plants

2011 ◽  
Vol 24 (5) ◽  
pp. 519-532 ◽  
Author(s):  
Sugihiro Ando ◽  
Yuko Sato ◽  
Hideyuki Shigemori ◽  
Takafumi Shimizu ◽  
Kazunori Okada ◽  
...  
Keyword(s):  
Rice Blast ◽  
Chemical Structure ◽  
Rice Blast Fungus ◽  
Conidial Suspension ◽  
Rice Plants ◽  
Blast Fungus ◽  
Plant Pathogen Interactions ◽  
Identification And Characterization ◽  
Compatible Interactions

We previously detected infection-promoting activity in the supernatant of the conidial suspension (SCS) of the rice blast fungus. In the present study, a molecule carrying the activity was purified and identified as 2′-deoxyuridine (dU). The infection-promoting activity of dU was strictly dependent on its chemical structure and displayed characteristics consistent with those of the SCS. Notably, the activity of dU was exclusively detected during interactions between rice and virulent isolates of the fungus, the number of susceptible lesions in leaf blades was increased by dU, and nonhost resistance in rice plants was not affected by treatment with dU. In addition, the expression of pathogensis-related genes, accumulation of H2O2, and production of phytoalexins in rice in response to inoculation with virulent fungal isolates was not suppressed by dU. The infection-promoting activity of dU was not accompanied by elevated levels of endogenous abscissic acid, which is known to modify plant-pathogen interactions, and was not detected in interactions between oat plants and a virulent oat blast fungus isolate. Taken together, these results demonstrate that dU is a novel infection-promoting factor that acts specifically during compatible interactions between rice plants and rice blast fungus in a mode distinct from that of toxins and suppressors.

Identification and characterization of five new OP2-related Myoviridae bacteriophages infecting different strains of Xanthomonas oryzae pv. oryzae

2018 ◽  
Vol 101 (2) ◽  
pp. 263-273 ◽  
Author(s):  
Solabomi Olaitan Ogunyemi ◽  
Jie Chen ◽  
Muchen Zhang ◽  
Li Wang ◽  
Md. Mahidul Islam Masum ◽  
...  
Keyword(s):  
Xanthomonas Oryzae ◽  
Different Strains ◽  
Identification And Characterization

Identification and characterization of peptidases secreted by quahog parasite unknown (QPX), the protistan parasite of hard clams

2016 ◽  
Vol 122 (1) ◽  
pp. 21-33 ◽  
Author(s):  
E Rubin ◽  
GT Werneburg ◽  
E Pales Espinosa ◽  
DG Thanassi ◽  
B Allam
Keyword(s):  
Quahog Parasite Unknown ◽  
Hard Clams ◽  
Identification And Characterization
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