Comparison of the bacterial viability assessments for the disinfected quarantined water along with an effect of total residual oxidants

2021 ◽  
Vol 193 (12) ◽  
Author(s):  
Hong Keun Yoon ◽  
Seon Yeong Park ◽  
Chang Gyun Kim
Keyword(s):  
2017 ◽  
Vol 5 (30) ◽  
pp. 6008-6015 ◽  
Author(s):  
Yuxiang Song ◽  
Hao Li ◽  
Fang Lu ◽  
Huibo Wang ◽  
Mengling Zhang ◽  
...  

Doped carbon dots from a yeast extract were first applied in real-time monitoring of bacterial viability as a nano-thermometer.


2015 ◽  
Vol 13 (1) ◽  
pp. 015602 ◽  
Author(s):  
P A Lukyanovich ◽  
B A Zon ◽  
M Yu Grabovich ◽  
E V Shchelukhina ◽  
I I Danilova ◽  
...  

3 Biotech ◽  
2021 ◽  
Vol 11 (6) ◽  
Author(s):  
Pablo Souza-Alonso ◽  
Miguel Rocha ◽  
Inês Rocha ◽  
Ying Ma ◽  
Helena Freitas ◽  
...  

2008 ◽  
Vol 78 (2) ◽  
Author(s):  
Utkur Mirsaidov ◽  
Winston Timp ◽  
Kaethe Timp ◽  
Mustafa Mir ◽  
Paul Matsudaira ◽  
...  

2018 ◽  
Author(s):  
Merilin Rosenberg ◽  
Nuno F. Azevedo ◽  
Angela Ivask

AbstractCombining membrane impermeable DNA-binding stain propidium iodide (PI) with membrane-permeable DNA-binding counterstains is a widely used approach for bacterial viability staining. In this paper we show that PI staining of adherent cells in biofilms may significantly underestimate bacterial viability due to the presence of extracellular nucleic acids. We demonstrate that gram-positive Staphylococcus epidermidis and gram-negative Escherichia coli 24-hour initial biofilms on glass consist of 76 and 96% PI-positive red cells in situ, respectively, even though 68% the cells of either species in these aggregates are metabolically active. Furthermore, 82% of E. coli and 89% S. epidermidis are cultivable after harvesting. Confocal laser scanning microscopy (CLSM) revealed that this false dead layer of red cells is due to a subpopulation of double-stained cells that have green interiors under red coating layer which hints at extracellular DNA (eDNA) being stained outside intact membranes. Therefore, viability staining results of adherent cells should always be validated by an alternative method for estimating viability, preferably by cultivation.


2005 ◽  
Vol 71 (3) ◽  
pp. 1662-1663 ◽  
Author(s):  
Sampo J. Lahtinen ◽  
Miguel Gueimonde ◽  
Arthur C. Ouwehand ◽  
Johanna P. Reinikainen ◽  
Seppo J. Salminen

ABSTRACT The determination of bacterial viability in probiotic products is of economic, technological, and clinical significance. We compared four methods to enumerate three Bifidobacterium strains in fermented oat products during storage. A subpopulation of nonculturable cells retained a functional cell membrane typical of viable cells, indicating that probiotic bacteria become dormant during storage.


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