scholarly journals Photoperiod-sensitive cytoplasmic male sterile elite lines for hybrid wheat breeding, showing high cross-pollination fertility under long-day conditions

Euphytica ◽  
2016 ◽  
Vol 212 (2) ◽  
pp. 313-322 ◽  
Author(s):  
K. Murai ◽  
H. Ohta ◽  
M. Kurushima ◽  
N. Ishikawa
Keyword(s):  
Wheat Breeding ◽  
Hybrid Wheat ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Cross Pollination ◽  
Long Day ◽  
Photoperiod Sensitive

Identification of genes related to the regulation of anther and pollen development in Mu-type cytoplasmic male sterile wheat (Triticum aestivum) by transcriptome analysis

2019 ◽  
Vol 70 (4) ◽  
pp. 306 ◽  
Author(s):  
Wei Li ◽  
Zihan Liu ◽  
Chang Meng ◽  
Yulin Jia ◽  
Lingli Zhang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Wheat Breeding ◽  
Abnormal Development ◽  
Nutrient Deficiencies ◽  
Hybrid Wheat ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Pectin Degradation ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Cytoplasmic male sterile (CMS) lines are important tools for hybrid production but they cannot produce viable pollen. Breeding new CMS lines and studying their sterility mechanism in wheat (Triticum aestivum L.) greatly facilitates the process of hybrid wheat breeding. We conducted transcriptome sequencing for a recently identified Mu-CMS line with Aegilops uniaristata Vis. cytoplasm, named U706A, and its isonuclear maintainer line (706B) at the binucleate stage, which was a critical period when abortion occurred. We found that most of the genes involved in phosphatidylinositol metabolism and pectin degradation were downregulated, as well as genes encoding the MYB21 and MYC2 transcription factors, in U706A compared with 706B. In addition, pectin contents indicated that the production of pectin has been enhanced from the binucleate stage to the trinucleate stage, owing to the downregulation of pectin-degradation-related genes in U706A at the binucleate stage, which confirmed the reliability of the sequencing results. We also discovered that the accumulation period of pectin content in U706A is abnormal compared with 706B, which may be an important reason for abortion. Some differentially expressed genes that might be related to the sterile phenotype were verified by quantitative RT-PCR. Therefore, we suggest that the downregulation of these genes possibly leads to the anther not to crack; the tapetum and microspore membrane system is less metabolised, and the abnormal pectin accumulation results in microspore nutrient deficiencies and abnormal development. These findings provide novel insights into the mechanism responsible for pollen abortion in CMS, which may facilitate hybrid wheat breeding.

scholarly journals Construction of a male sterility system for hybrid rice breeding and seed production using a nuclear male sterility gene

2016 ◽  
Vol 113 (49) ◽  
pp. 14145-14150 ◽  
Author(s):  
Zhenyi Chang ◽  
Zhufeng Chen ◽  
Na Wang ◽  
Gang Xie ◽  
Jiawei Lu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Environmental Conditions ◽  
Hybrid Rice ◽  
Rice Breeding ◽  
Male Sterile ◽  
Red Fluorescence ◽  
Cytoplasmic Male Sterile ◽  
Cross Pollination ◽  
Self Pollination

The breeding and large-scale adoption of hybrid seeds is an important achievement in agriculture. Rice hybrid seed production uses cytoplasmic male sterile lines or photoperiod/thermo-sensitive genic male sterile lines (PTGMS) as female parent. Cytoplasmic male sterile lines are propagated via cross-pollination by corresponding maintainer lines, whereas PTGMS lines are propagated via self-pollination under environmental conditions restoring male fertility. Despite huge successes, both systems have their intrinsic drawbacks. Here, we constructed a rice male sterility system using a nuclear gene named Oryza sativa No Pollen 1 (OsNP1). OsNP1 encodes a putative glucose–methanol–choline oxidoreductase regulating tapetum degeneration and pollen exine formation; it is specifically expressed in the tapetum and miscrospores. The osnp1 mutant plant displays normal vegetative growth but complete male sterility insensitive to environmental conditions. OsNP1 was coupled with an α-amylase gene to devitalize transgenic pollen and the red fluorescence protein (DsRed) gene to mark transgenic seed and transformed into the osnp1 mutant. Self-pollination of the transgenic plant carrying a single hemizygous transgene produced nontransgenic male sterile and transgenic fertile seeds in 1:1 ratio that can be sorted out based on the red fluorescence coded by DsRed. Cross-pollination of the fertile transgenic plants to the nontransgenic male sterile plants propagated the male sterile seeds of high purity. The male sterile line was crossed with ∼1,200 individual rice germplasms available. Approximately 85% of the F1s outperformed their parents in per plant yield, and 10% out-yielded the best local cultivars, indicating that the technology is promising in hybrid rice breeding and production.

scholarly journals Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding

2020 ◽  
Author(s):  
Hongsheng Li ◽  
Shaoxiang Li ◽  
Sedhom Abdelkhalik ◽  
Armaghan Shahzad ◽  
Jian Gu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Doubled Haploid ◽  
Yellow Rust ◽  
Wheat Breeding ◽  
Yield Potential ◽  
Limiting Factor ◽  
Hybrid Wheat ◽  
Male Sterile ◽  
Dh Lines ◽  
F2 Generation

Abstract Background: Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past twenty years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids. Application of doubled haploid (DH) breeding provides a useful strategy to efficiently develop practically usable TPSGMS lines. Results: F1s and selected F2 and F3 sterile plants of eight crosses made from two commercial TPSGMS lines were used to produce DH lines. We developed a total of 24 elite DH sterile lines with stable sterility, good outcrossing and yield potential, resistance to yellow rust and powdery mildew, as well as desirable plant height (50-60 cm). These DH lines were developed within 4 years through at least one year of evaluation. The stability of male sterility was confirmed for most (20/24) of these elite DH sterile lines by multiple tests in two or three years. These lines are expected to be used in hybrid wheat breeding. The percentage of elite lines developed from the tested DH lines produced from filial generations was in the order of F2 > F3 > F1. Conclusions: We demonstrate that coupling DH techniques with conventional breeding is an efficient strategy for accelerating the development of more practical wheat TPSGMS lines. Generation of DHs from F2 generation appeared to be the better choice considering the balance of shortening breeding time and overall breeding efficiency.

scholarly journals Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding

2020 ◽  
Author(s):  
Hongsheng Li ◽  
Shaoxiang Li ◽  
Sedhom Abdelkhalik ◽  
Armaghan Shahzad ◽  
Jian Gu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Doubled Haploid ◽  
Yellow Rust ◽  
Wheat Breeding ◽  
Yield Potential ◽  
Limiting Factor ◽  
Hybrid Wheat ◽  
Male Sterile ◽  
One Year ◽  
Dh Lines

Abstract Background: Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past twenty years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids. Application of doubled haploid (DH) breeding provides a useful strategy to efficiently develop practically usable TPSGMS lines.Results: F1s and selected F2 and F3 sterile plants of eight crosses made from two commercial TPSGMS lines were used to produce DH lines. We developed a total of 24 elite DH sterile lines with stable sterility, good outcrossing and yield potential, resistance to yellow rust and powdery mildew, as well as desirable plant height (50-60 cm). These DH lines were developed within 4 years through at least one year of evaluation. The stability of male sterility was confirmed for most (20/24) of these elite DH sterile lines by multiple tests in two or three years. These lines are expected to be used in hybrid wheat breeding. The percentage of elite lines developed from the tested DH lines produced from filial generations was in the order of F2 > F3 > F1.Conclusions: We demonstrate that coupling DH techniques with conventional breeding is an efficient strategy for accelerating the development of more practical wheat TPSGMS lines. Generation of DHs from F2 generation appeared to be the better choice considering the balance of shortening breeding time and overall breeding efficiency.

scholarly journals Floral traits and flowering behaviors of Malaysian rice cytoplasmic male sterile and maintainer lines and its relationship with out-crossing performance

2021 ◽  
pp. 180-186
Author(s):  
Shahida Hashim ◽  
Phebe Ding ◽  
Mohd Firdaus Ismail ◽  
Asfaliza Ramli
Keyword(s):  
Seed Production ◽  
Hybrid Rice ◽  
Agricultural Research ◽  
Floral Traits ◽  
Rice Seed ◽  
Male Sterile ◽  
Floral Trait ◽  
Cytoplasmic Male Sterile ◽  
Cross Pollination ◽  
Parental Lines

Rice is a strictly self-pollinating crop. However, in hybrid rice seed production, an effective male sterility system is used to produce hybrid seed in bulk. In hybrid rice system, the pollen grains of cytoplasmic male sterile (CMS) are sterile and the female organ of the CMS depends on the fertile pollen released by the maintainer or restorer lines via out-crossing or cross-pollination in order to produce seed. Floral trait and flowering behavior of CMS and its corresponding maintainer or restorer lines are essential factors in hybrid rice seed production because they influenced the out-crossing or cross-pollination between parental lines. Two local CMSs and their corresponding maintainer lines were developed through breeding program in Malaysian Agricultural Research and Development Institute (MARDI) namely 0025A/0025B and 0047A/0047B. This study was carried out on floral traits and flowering behavior of these two hybrid line. Present studies have shown that there were variations between the CMS and its maintainer lines whether on floral trait or flowering behavior for both hybrid rice combinations. The results showed that stigma characters for both 0025A and 0047 were superior than their respective maintainers. Therefore, it is expected that the out-crossing rate would be high. Seeding date intervals need to be done on 0025A/0025B during nursery stage because the on-set of flowering between parental lines was significantly different. Panicle of both CMS was also classified as just exserted and partially-exserted and application of exogenous hormones such as gibberellic acid was useful to improve panicle elongation and consequently increase the seed set and yield. Correlation study indicates that the stigma area of both 0025A and 0047A has significant positive correlation with out-crossing rate.

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