Sequence requirement of the ade6-4095 meiotic recombination hotspot in Schizosaccharomyces pombe

Genetica ◽  
2017 ◽  
Vol 146 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Steven J. Foulis ◽  
Kyle R. Fowler ◽  
Walter W. Steiner
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Recombination Hotspot

scholarly journals Active and inactive transplacement of the M26 recombination hotspot in Schizosaccharomyces pombe.

Genetics ◽  
1995 ◽  
Vol 141 (1) ◽  
pp. 33-48
Author(s):  
J B Virgin ◽  
J Metzger ◽  
G R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Plasmid Dna ◽  
Context Effect ◽  
Intragenic Recombination ◽  
Multicopy Plasmid ◽  
Chromosomal Locus ◽  
Sequence Element ◽  
Recombination Hotspot ◽  
Recombination Hotspots

Abstract The ade6-M26 mutation of the fission yeast Schizosaccharomyces pombe creates a meiotic recombination hotspot that elevates ade6 intragenic recombination approximately 10-15-fold. A heptanucleotide sequence including the M26 point mutation is required but not sufficient for hotspot activity. We studied the effects of plasmid and chromosomal context on M26 hotspot activity. The M26 hotspot was inactive on a multicopy plasmid containing M26 embedded within 3.0 or 5.9 kb of ade6 DNA. Random S. pombe genomic fragments totaling approximately 7 Mb did not activate the M26 hotspot on a plasmid. M26 hotspot activity was maintained when 3.0-, 4.4-, and 5.9-kb ade6-M26 DNA fragments, with various amounts of non-S. pombe plasmid DNA, were integrated at the ura4 chromosomal locus, but only in certain configurations relative to the ura4 gene and the cointegrated plasmid DNA. Several integrations created new M26-independent recombination hotspots. In all cases the non-ade6 DNA was located > 1 kb from the M26 site, and in some cases > 2 kb. Because the chromosomal context effect was transmitted over large distances, and did not appear to be mediated by a single discrete DNA sequence element, we infer that the local chromatin structure has a pronounced effect on M26 hotspot activity.

scholarly journals Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe.

Genetics ◽  
1988 ◽  
Vol 119 (3) ◽  
pp. 491-497
Author(s):  
A S Ponticelli ◽  
E P Sena ◽  
G R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Base Pair ◽  
Initiation Site ◽  
Mitotic Recombination ◽  
Physical Analysis ◽  
Nucleotide Change ◽  
Recombination Hotspot ◽  
Base Pair Mismatch

Abstract The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate ade6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G----T nucleotide change, that M26 stimulated recombination within ade6 but not at other distinct loci, and that M26 stimulated meiotic but not mitotic recombination. In addition, M26 stimulated recombination within ade6 when M26 is homozygous; this result demonstrates that a base-pair mismatch at the M26 site was not required for the stimulation. These results are consistent with the ade6-M26 mutation creating a meiotic recombination initiation site.

scholarly journals A Family of cAMP-Response-Element-Related DNA Sequences With Meiotic Recombination Hotspot Activity in Schizosaccharomyces pombe

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 59-68
Author(s):  
Mary E Fox ◽  
Takatomi Yamada ◽  
Kunihiro Ohta ◽  
Gerald R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Dna Sequences ◽  
Meiotic Recombination ◽  
Consensus Sequence ◽  
Micrococcal Nuclease ◽  
Open Chromatin ◽  
Camp Response Element ◽  
Response Element ◽  
Recombination Hotspot ◽  
Single Sequence

Abstract The heptamer sequence ATGACGT is essential for activity of the M26 meiotic recombination hotspot in the ade6 gene of Schizosaccharomyces pombe. Hotspot activity is associated with binding of the heterodimeric transcription factor Atf1·Pcr1 to M26. We have found that the sequences (C/T/G) TGACGT also bound Atf1·Pcr1 and acted as meiotic hotspots, but unlike M26 they must be followed by A or C for Atf1·Pcr1 binding and hotspot activity. The basis of the hotspot activity of CTGACGTA (ade6-3013) appears to be identical to that of M26: hotspot activity of both sequences was abolished in cells mutant for atf1, pcr1, spc1, or wis1 and was undetectable in mitotic recombination and in meiotic recombination when located on a plasmid. Both hotspot sequences were sites of micrococcal nuclease hypersensitivity in meiotic chromatin, suggesting that they create an open chromatin structure during meiosis at the site of the hotspots. The newly identified hotspot sequences (C/T/G)TGACGT(A/C) and M26 are closely related to the cAMP response element (CRE) consensus sequence for binding of cAMP-responsive transcription factors such as Atf1·Pcr1, suggesting a link between transcription and meiotic recombination. These results significantly expand the list of identified sequences with meiotic recombination hotspot activity in S. pombe from a single sequence to a family of CRE-related sequences.

scholarly journals Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.

Genetics ◽  
1989 ◽  
Vol 123 (1) ◽  
pp. 45-54 ◽  
Author(s):  
A S Ponticelli ◽  
G R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Intragenic Recombination ◽  
Gene Products ◽  
Recombination Hotspot ◽  
Mutant Screen ◽  
Complementation Groups ◽  
Chromosome Iii ◽  
Rec Genes ◽  
Chromosome I

Abstract A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than or equal to 300-fold when homozygous. Three recessive rec mutations analyzed further also reduced meiotic intragenic recombination at ura4 on chromosome III and intergenic recombination between pro2 and arg3 on chromosome I. The observed non-co-ordinate reductions of the recombinant frequencies in the three test intervals suggest a degree of locus (or intragenic vs. intergenic) specificity of the corresponding rec+ gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods.

scholarly journals Fine-Structure Mapping of Meiosis-Specific Double-Strand DNA Breaks at a Recombination Hotspot Associated With an Insertion of Telomeric Sequences Upstream of the HIS4 Locus in Yeast

Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1115-1125 ◽  
Author(s):  
Fei Xu ◽  
Thomas D Petes
Keyword(s):  
Meiotic Recombination ◽  
Hydroxyl Groups ◽  
Sequence Specificity ◽  
Structure Mapping ◽  
Dna Breaks ◽  
Exonuclease Iii ◽  
Recombination Hotspot ◽  
Telomeric Sequences ◽  
Double Strand Dna Breaks ◽  
Double Strand Dna

Abstract Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of ~50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3′-hydroxyl groups.

scholarly journals A 160-bp Palindrome Is a Rad50·Rad32-Dependent Mitotic Recombination Hotspot inSchizosaccharomyces pombe

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 461-468 ◽  
Author(s):  
Joseph A Farah ◽  
Edgar Hartsuiker ◽  
Ken-ichi Mizuno ◽  
Kunihiro Ohta ◽  
Gerald R Smith
Keyword(s):  
Secondary Structure ◽  
Schizosaccharomyces Pombe ◽  
Inverted Repeat ◽  
Mitotic Recombination ◽  
Genome Integrity ◽  
Recombination Hotspot ◽  
Nuclease Domain ◽  
Palindromic Sequences

AbstractPalindromic sequences can form hairpin and cruciform structures that pose a threat to genome integrity. We found that a 160-bp palindrome (an inverted repeat of 80 bp) conferred a mitotic recombination hotspot relative to a control nonpalindromic sequence when inserted into the ade6 gene of Schizosaccharomyces pombe. The hotspot activity of the palindrome, but not the basal level of recombination, was abolished by a rad50 deletion, by a rad50S “separation of function” mutation, or by a rad32-D25A mutation in the nuclease domain of the Rad32 protein, an Mre11 homolog. We propose that upon extrusion of the palindrome the Rad50·Rad32 nuclease complex recognizes and cleaves the secondary structure thus formed and generates a recombinogenic break in the DNA.

Region‐specific meiotic recombination in Schizosaccharomyces pombe : the rec11 gene

1997 ◽  
Vol 23 (5) ◽  
pp. 869-878 ◽  
Author(s):  
Ywan Feng Li ◽  
Masayuki Numata ◽  
Wayne P. Wahls ◽  
Gerald R. Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination
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