Bone induction and defect repair by true bone ceramics incorporated with rhBMP-2 and Sr

AbstractObjective: To study the bone induction and defect repair of true bone ceramics (TBC) combined with rhBMP-2 and Sr. Methods: MC3T3-E1 cells were used to evaluate the bioactivity of the composite. Cell proliferation activity was detected by CCK-8, ALP activity was detected by p-nitrophenyl phosphate (PNPP), and the differences of material surface topography were observed by scanning electron microscopy (SEM). Bone induction was verified by the implantation in nude mice. The rabbit femoral condyle defect model was achieved to verify the bone defect repair ability of the material. Results: SEM results showed nearly the same surface morphology and cell proliferation quantified by CCK-8 showed that compared with TBC, both TBC&Sr and TBC&BMP-2&Sr had a significant promoting effect (P < 0.05). ALP activity result showed that the ALP activity of TBC&BMP-2&Sr was significantly higher than that of TBC alone (P < 0.05). The bone induction result showed that TBC&Sr had a small amount of new bone formation, and the new bone area was only 2.5 ± 0.11%. The bone induction activity of TBC&BMP-2&Sr was the highest, the new bone area was up to 75.36 ± 4.21%. Histological result of bone defect repair showed that TBC&BMP-2&Sr was also the highest, the new bone area was up to 72.42 ± 3.14%. The repair effect of TBC& BMP-2 was second, and better than that of TBC&Sr. Conclusion: TBC combined with rhBMP-2 and Sr had the good bioactivity, obvious bone conduction and bone defect repair performance, laying the foundation of clinical application potentially.

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miR-196a-5p Promoted the Osteogenic Differentiation and Calvarial Bone Defect Repair of Wharton’s Jelly Umbilical Cord Stem Cells

10.21203/rs.3.rs-962654/v1 ◽

2021 ◽

Author(s):

Yantong Wang ◽

Simin Zhang ◽

Haoqing Yang ◽

Yangyang Cao ◽

Dianqin Yu ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Bone Defect ◽

Bone Repair ◽

Calvarial Bone ◽

Bone Defect Repair ◽

Alp Activity ◽

Defect Repair ◽

Umbilical Cord Stem Cells ◽

Calvarial Bone Defect

Abstract Background: To investigate the effect of miR‐196a-5p on the osteogenic differentiation and defected bone repair of Wharton’s jelly umbilical cord stem cells (WJCMSCs). Methods: miR‐196a-5p mimic or inhibitor was applied to overexpress or silence miR‐196a-5p expression in WJCMSCs. The alkaline phosphatase (ALP) activity, mineralization ability, and osteogenic markers expression were used to test WJCMSCs osteogenic potential in vitro. Calvarial bone defect model of rat was used to evaluate WJCMSCs bone regeneration ability in vivo. mRNA microarray was used to reveal the underling mechanisms that miR‐196a-5p regulated bone repair.Results: miR-196a-5p inhibition reduced the ALP activity, mineralization ability, and level of osteogenic markers OCN, DSPP, DMP1 and BSP, while miR-196a-5p overexpression enhanced the ALP activity, mineralization ability, and level of OCN, DSPP, DMP1 and BSP of WJCMSCs in vitro. Next, the micro-CT and histopathology results showed miR-196a-5p-overexpressed-WJCMSCs obviously promoted the new bone tissue regeneration and calvarial bone defect repair after MSCs transplanted for 12 weeks. Further, mRNA microarray of miR-196a-5p-overexpressed-WJCMSCs revealed totally 959 significantly differentially expressed genes (DEGs), among which 34 upregulated and 925 downregulated. Also, 241 miR-196a-5p targeted genes were predicted by using miRNA targeted websites and only 19 predicted genes were consistent with microarray results. On this basis, one significantly downregulated gene SERPINB2 was selected and revealed that SERPINB2 deletion obviously enhanced the ALP activity and mineralization ability of WJCMSCs in vitro.Conclusions: miR-196a-5p promoted the osteogenic differentiation potential and calvarial bone defect repair ability of WJCMSCs. And SERPINB2 acted as one key downstream gene to participate in the miR-196a-5p promoted MSCs osteogenic differentiation.

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Design and characterization of injectable abalone shell/calcium sulfate bone cement scaffold for bone defect repair

Chemical Engineering Journal ◽

10.1016/j.cej.2021.129866 ◽

2021 ◽

Vol 420 ◽

pp. 129866

Author(s):

Mingzu Du ◽

Qian Li ◽

Jingdi Chen ◽

Kaihua Liu ◽

Cui Song

Keyword(s):

Bone Cement ◽

Bone Defect ◽

Calcium Sulfate ◽

Bone Defect Repair ◽

Defect Repair ◽

Abalone Shell

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Bone defect repair on the alveolar wall of the maxillary sinus using collagen membranes and temporal fascia: an experimental study in monkeys

Brazilian Journal of Otorhinolaryngology ◽

10.1590/s1808-86942011000400006 ◽

2011 ◽

Vol 77 (4) ◽

pp. 439-446 ◽

Cited By ~ 1

Author(s):

Adalberto Novaes Silva ◽

José Américo de Oliveira ◽

Maria Célia Jamur ◽

José Ari Gualberto Junqueira ◽

Vani Maria Correa ◽

...

Keyword(s):

Experimental Study ◽

Maxillary Sinus ◽

Bone Defect ◽

Alveolar Wall ◽

Bone Defect Repair ◽

Temporal Fascia ◽

Defect Repair ◽

Collagen Membranes

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Clay-based nanocomposite hydrogel with attractive mechanical properties and sustained bioactive ion release for bone defect repair

Journal of Materials Chemistry B ◽

10.1039/d1tb00184a ◽

2021 ◽

Author(s):

Xinyun Zhai ◽

Changshun Ruan ◽

Jie Shen ◽

Chuping Zheng ◽

Xiaoli Zhao ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Defect ◽

Nanocomposite Hydrogel ◽

Ion Release ◽

Bone Defect Repair ◽

Defect Repair ◽

Gradual Release

Using nanoclay as the physical crosslinker, a novel clay-based nanocomposite hydrogel with attractive mechanical properties has be obtained, and the gradual release of intrinsic Mg2+ and Si4+ endows the system with excellent osteogenesis.

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Injectable gel graft for bone defect repair

Regenerative Medicine ◽

10.2217/rme.13.76 ◽

2014 ◽

Vol 9 (1) ◽

pp. 41-51 ◽

Cited By ~ 13

Author(s):

Josephine Fang ◽

Zhi Yang ◽

ShihJye Tan ◽

Charisse Tayag ◽

Marcel E Nimni ◽

...

Keyword(s):

Bone Defect ◽

Bone Defect Repair ◽

Defect Repair

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Nano-Hydroxyapatite Scaffold Based on Recombinant Human Bone Morphogenetic Protein 2 and Its Application in Bone Defect Repair

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3110 ◽

2021 ◽

Vol 17 (7) ◽

pp. 1330-1338

Author(s):

Shibai Zhu ◽

Xiaotian Zhang ◽

Xi Chen ◽

Yiou Wang ◽

Shanni Li ◽

...

Keyword(s):

Bone Defect ◽

Bone Defects ◽

Bone Morphogenetic Protein 2 ◽

Artificial Bone ◽

Bone Defect Repair ◽

Morphogenetic Protein ◽

Human Bone Morphogenetic Protein ◽

Defect Repair ◽

Hydroxyapatite Scaffold ◽

Recombinant Human Bone

The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 μL glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In in vivo experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.

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