A Series of Carbazole Cationic Compounds with Large Two-Photon Absorption Cross Sections for Imaging Mitochondria in Living Cells with Two-Photon Fluorescence Microscopy

2010 ◽  
Vol 21 (2) ◽  
pp. 497-506 ◽  
Author(s):  
Xin Liu ◽  
Yuming Sun ◽  
Yuanhong Zhang ◽  
Ning Zhao ◽  
Hongshi Zhao ◽  
...  
2019 ◽  
Vol 955 ◽  
pp. 37-43
Author(s):  
Martin Vala ◽  
Matouš Kratochvíl ◽  
Patricie Heinrichova ◽  
Martin Weiter

The paper deals with determination of two-photon absorption cross-sections of selected representatives of diketo-pyrrolo-pyrrole. The materials were optimized in order to test the influence of various substitutions on the diketo-pyrrolo-pyrrole basic molecule. The two-photon absorption cross-sections were determined using two-photon excited fluorescence technique. A considerably high two-photon absorption cross-section in order of 1000 GM was found for non-symmetrically substituted derivative with donor-acceptor structure. All of the derivatives showed strong fluorescence in solid state visible by naked eye. These results demonstrate that this class of materials can be used in e.g. two-photon fluorescence microscopy in the form of nanoparticles.


Absolute values of the two-photon absorption cross-sections of Rhodamine 6G, Rhodamine B, Acridine Red, Disodium Fluorescein and DODCI have been obtained by excitation with a neodymium: glass laser (1060 nm). Measurements over eight orders of magnitude of the exciting light flux were carried out by employing the laser both Q-switched and mode-locked. The two-photon fluorescence flux measurements were calibrated by single-photon fluorescence generated by excitation with second-harmonic laser pulses. The experimental results are compared with values predicted by semi-empirical theoretical models and good agreement is obtained. Two-photon amplification of the mode-locked neodymium laser by organic dyes is shown not to be practicable at present. Other possible two-photon oscillators are discussed.


2010 ◽  
Vol 97 (17) ◽  
pp. 173703 ◽  
Author(s):  
Shen Zheng ◽  
Ji-Yao Chen ◽  
Pei-Nan Wang ◽  
Wu-Li Yang ◽  
Lu-Wei Zhou

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