Fluorescence Spectroscopy and Confocal Microscopy of the Mycotoxin Citrinin in Condensed Phase and Hydrogel Films

2014 ◽  
Author(s):  
Milena H. Lauer ◽  
Marcelo H. Gehlen ◽  
Karen de Jesus ◽  
Roberto G. S. Berlinck
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Spectroscopy ◽  
Condensed Phase ◽  
Hydrogel Films

Synthesis and photophysical properties of conjugated (dodecyl)benzoateethynylene macromolecules: staining ofBacillus subtilisandEscherichia colirhizobacteria

2019 ◽  
Vol 43 (8) ◽  
pp. 3332-3340 ◽  
Author(s):  
Ma. Concepción García ◽  
Gleb Turlakov ◽  
Ivana Moggio ◽  
Eduardo Arias ◽  
J. Humberto Valenzuela ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Spectroscopy ◽  
Photophysical Properties ◽  
Laser Confocal Microscopy ◽  
Spectroscopy Laser

The staining of agrobacteria was successfully demonstrated through a benzoateethynylene by fluorescence spectroscopy, laser confocal microscopy and microRaman.

Applications of Fluorescence Spectroscopy and Confocal Microscopy

2011 ◽  
pp. 235-272 ◽  
Author(s):  
Anya Salih ◽  
Paul Wormell ◽  
K. Benjamin Garbutcheon-Singh ◽  
Benjamin Harper ◽  
Simon Myers ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Spectroscopy

Structure–property relationships in two-dimensionally extended benzoporphyrin molecules probed using single-molecule fluorescence spectroscopy

2016 ◽  
Vol 18 (10) ◽  
pp. 7521-7526 ◽  
Author(s):  
Sujin Ham ◽  
Sang Hyeon Lee ◽  
Heejae Chung ◽  
Dongho Kim
Keyword(s):  
Fluorescence Spectroscopy ◽  
Single Molecule ◽  
Condensed Phase ◽  
Photophysical Properties ◽  
Structure Property ◽  
Single Molecule Fluorescence ◽  
Structure Property Relationships ◽  
Single Molecule Fluorescence Spectroscopy ◽  
Different Shapes

The photophysical properties of a series of highly π-conjugated benzoporphyrin molecules (BPNs) with different shapes were investigated in the condensed phase using single-molecule fluorescence spectroscopy.

Confocal microscopy: an experimental set up for biomolecule structure investigation based on dynamical fluorescence spectroscopy

2003 ◽  
Author(s):  
Amanda Oldani ◽  
Maddalena Collini ◽  
Giuseppe Chirico ◽  
Laura D'Alfonso ◽  
Giancarlo Baldini ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Spectroscopy ◽  
Structure Investigation ◽  
Set Up

In situ optical spectroscopy of some systems of biological interest

1988 ◽  
Vol 8 (6) ◽  
pp. 497-508
Author(s):  
D. Balasubramanian
Keyword(s):  
Optical Absorption ◽  
Fluorescence Spectroscopy ◽  
Condensed Phase ◽  
Supramolecular Assembly ◽  
Eye Lens ◽  
Biological Interest ◽  
The Status ◽  
Phase Sample ◽  
Packing Effects

Monitoring the optical absorption or emission spectrum of a condensed phase sample offers information about the supramolecular assembly, packing effects and other features characteristic of the phase that would be missed when one studies solution-state spectra. We have used the technique of photoacoustic spectroscopy to study intact biological specimens, such as algae, parasite cells and the eye lens. Such a study has offered information about the status of endogenous hemin in Plasmodium cells and the mode of interaction of antimalarial drugs of the chloroquine class therein. We have also attempted to do in situ fluorescence spectroscopy on isolated intact eye lenses, which has enabled us to follow the photochemistry and the status of the photoproduct of the oxidation of the trp residues of the crystallins of the lens.

Double Resonance Excitation of Fluorescence in the Condensed Phase—An Alternative to Infrared, Raman, and Fluorescence Spectroscopy

1980 ◽  
Vol 34 (2) ◽  
pp. 151-157 ◽  
Author(s):  
John C. Wright
Keyword(s):  
Fluorescence Spectroscopy ◽  
Raman Scattering ◽  
Condensed Phase ◽  
Stimulated Raman Scattering ◽  
Vibrational Excitation ◽  
Double Resonance ◽  
Excitation Frequency ◽  
Resonance Excitation ◽  
Relaxation Rates ◽  
Stimulated Raman

The feasibility of performing a double resonance excitation of fluorescence from molecules in a condensed phase is discussed. A vibrational excitation can be created by either stimulated Raman scattering or infrared absorption while a second excitation can excite the molecule to an excited electronic state from which fluorescence can occur. By providing high rates of excitation, one can overcome the rapid vibrational relaxation rates to produce fluorescence. By scanning the second excitation frequency in synchronization with either the infrared frequency or the difference frequency producing the stimulated Raman scattering, one can obtain spectra that are analogous to Raman or infrared spectra. This approach would have the sharp lines characteristic of Raman or infrared spectroscopy but would have an increased sensitivity characteristic of fluorescence spectroscopy.

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

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