Fluorescence Enhancement Effect for the Determination of Nucleic Acids With Morin–NanoTiO2

2011 ◽  
Vol 21 (4) ◽  
pp. 881-885
Author(s):  
Kang Du ◽  
Weihua Liu ◽  
Shuang Qiao ◽  
Changli Zhou ◽  
Wei Cao
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Enhancement ◽  
Enhancement Effect

Determination of Nucleic Acids at Nanogram Levelsby their Enhancement Effect on the Rateof the Metalloporphyrin-Catalyzed p -HydroxyphenylaceticAcid Fluorescence Reaction

2002 ◽  
Vol 140 (3-4) ◽  
pp. 241-246 ◽  
Author(s):  
Xiao-Lan Chen ◽  
Zong-Bao Li ◽  
Ling Wei ◽  
Hui-Hing Qu ◽  
Jin-Gou Xu
Keyword(s):  
Nucleic Acids ◽  
Enhancement Effect ◽  
Fluorescence Reaction

The Sensitive Determination of Nucleic Acids Using Fluorescence Enhancement of Eu3+-BenzoylacetoneCetyltrimethylammonium Bromide-Nucleic Acid System

2005 ◽  
Vol 15 (5) ◽  
pp. 655-660 ◽  
Author(s):  
Xia Wu ◽  
Changying Guo ◽  
Jinghe Yang ◽  
Minqin Wang ◽  
Yanjing Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Fluorescence Enhancement ◽  
Acid System ◽  
Sensitive Determination

Fluorescence enhancement effect of morin-nucleic acid-l-cysteine-capped nano-ZnS system and the determination of nucleic acid

The Analyst ◽  
2008 ◽  
Vol 133 (7) ◽  
pp. 896 ◽  
Author(s):  
Liangliang Zhao ◽  
Xia Wu ◽  
Honghong Ding ◽  
Jinghe Yang
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Enhancement ◽  
Enhancement Effect

DNA—Dye Fluorescence Enhancement Based on Shifting the Dimer—Monomer Equilibrium of Fluorescent Dye

1997 ◽  
Vol 51 (7) ◽  
pp. 1002-1007 ◽  
Author(s):  
Xiang-Qun Guo ◽  
Zu-Lin Zhang ◽  
Yi-Bing Zhao ◽  
Dong-Yuan Wang ◽  
Jin-Gou Xu
Keyword(s):  
Nucleic Acids ◽  
Acridine Orange ◽  
Anionic Surfactant ◽  
Fluorescence Enhancement ◽  
Sodium Dodecyl ◽  
Fluorescent Dye ◽  
Enhancement Effect ◽  
Solution Intercalation ◽  
Dye Fluorescence ◽  
Ct Dna

In this paper, the investigation of DNA–dye fluorescence enhancement based on shifting the dimer–monomer equilibrium of a fluorescent dye, acridine orange (AO), is reported. Formation of a virtually nonfluorescent dimeric dye, acridine orange homodimer (AOAO), induced by the pre-micellar aggregation of an anionic surfactant, sodium dodecyl sulfate (SDS), was observed. The possibility of using the in situ formed AOAO as a fluorescent probe for nucleic acids and polynucleotides was studied. The results showed that a nearly 1000-fold fluorescence enhancement was observed upon addition of calf thymus DNA (CT DNA). The fluorescence enhancement effect of DNA was thought to be based on the DNA modulated shift of the dimmer monomer equilibrium of AO in the anionic surfactant solution. Intercalation of the monomer in DNA caused the dissociation of AOAO and led to a very high fluorescence enhancement. It seemed that the dimeric dye molecules acted as a source of monomer molecules ready for interacting with nucleic acids and, at the same time, decreased the inherent fluorescence of monomer molecules, which proved to be unfavorable to the detection of fluorescence enhancement. A linear dependence of fluorescence intensity on CT DNA concentration over a range from 7.8 ng/mL to 10.0 g/mL, in the presence of AO at a concentration of 1.65 × 10−6mol/L and of SDS at a concentration of 8.0 × 10−4 mol/L, allowed sensitive quantitation of CT DNA in a conventional fluorometer. Calibration graphs for yeast RNA and polynucleotides, such as poly A, poly U, and poly I, were also obtained.

Fluorescence enhancement effect for the determination of proteins with morin–Al–cetyltrimethylammonium bromide

Talanta ◽  
2005 ◽  
Vol 67 (4) ◽  
pp. 836-842 ◽  
Author(s):  
F WANG ◽  
J YANG ◽  
X WU ◽  
C SUN ◽  
S LIU ◽  
...  
Keyword(s):  
Cetyltrimethylammonium Bromide ◽  
Fluorescence Enhancement ◽  
Enhancement Effect

Fluorescence enhancement effect for the determination of DNA with calcein–cetyl trimethyl ammonium bromide system

2008 ◽  
Vol 625 (2) ◽  
pp. 195-200 ◽  
Author(s):  
Fengshan Yu ◽  
Yanbin Ding ◽  
Yongwei Gao ◽  
Shishi Zheng ◽  
Fang Chen
Keyword(s):  
Cetyl Trimethyl Ammonium Bromide ◽  
Fluorescence Enhancement ◽  
Ammonium Bromide ◽  
Enhancement Effect ◽  
Cetyl Trimethyl Ammonium
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