Fifteen tests carried out at an average interval of 4 weeks for 14 months showed an annual rhythm in the proportion of spayed crossbred ewes which exhibited oestrus after an injection of 15.6 µg oestradiol benzoate (ODB) following a 12 day period of progesterone treatment. Reactivity was highest in late summer and early autumn and lowest in winter and early spring. The periods of minimum reactivity coincided with the periods of low environmental temperatures and low body weights. The significance of the annual rhythm is discussed with particular relation to the phenomenon of "silent oestrus". No similar rhythm was detected in the 7-aginal response to oestrogen.