Genome Analysis of Bacillus aryabhattai to Identify Biosynthetic Gene Clusters and In Silico Methods to Elucidate its Antimicrobial Nature

2021 ◽  
Author(s):  
Deepak A. Yaraguppi ◽  
Sanjay H. Deshpande ◽  
Zabin K. Bagewadi ◽  
Sudeep Kumar ◽  
Uday M. Muddapur
Keyword(s):  
Genome Analysis ◽  
In Silico ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Bacillus Aryabhattai ◽  
In Silico Methods

scholarly journals In silico exploration of Red Sea Bacillus genomes for natural product biosynthetic gene clusters

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Ghofran Othoum ◽  
Salim Bougouffa ◽  
Rozaimi Razali ◽  
Ameerah Bokhari ◽  
Soha Alamoudi ◽  
...  
Keyword(s):  
Natural Product ◽  
Red Sea ◽  
In Silico ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters

In silico genome mining of potential novel biosynthetic gene clusters for drug discovery from Burkholderia bacteria

2021 ◽  
pp. 105046
Author(s):  
Khorshed Alam ◽  
Md Mahmudul Islam ◽  
Kai Gong ◽  
Muhammad Nazeer Abbasi ◽  
Ruijuan Li ◽  
...  
Keyword(s):  
Drug Discovery ◽  
In Silico ◽  
Genome Mining ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters

scholarly journals Genome Analysis of Streptomyces nojiriensis JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus Streptomyces

2021 ◽  
Vol 9 (9) ◽  
pp. 1802
Author(s):  
Jin-Soo Park ◽  
Da-Eun Kim ◽  
Sung-Chul Hong ◽  
Seung-Young Kim ◽  
Hak Cheol Kwon ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Chemical Profile ◽  
Biosynthetic Gene Clusters ◽  
Genus Streptomyces ◽  
Streptomyces Spp ◽  
Spectroscopic Analyses ◽  
Different Types ◽  
Different Strains

Streptomyces spp. have been major contributors of novel natural products that are used in many application areas. We found that the nojirimycin (NJ) producer JCM 3382 has antimicrobial activity against Staphylococcus aureus via cellular degradation. Genome analysis revealed 30 biosynthetic gene clusters, including those responsible for producing antibiotics, including an azasugar NJ. In-depth MS/MS analysis confirmed the production of 1-deoxynojirimycin (DNJ) along with NJ. In addition, the production of tambromycins, setomimycin, and linearmycins was verified by spectroscopic analyses, including LC-MS and NMR. The distribution of the clusters of genes coding for antibiotics in 2061 Streptomyces genomes suggested potential producers of tambromycin, setomimycin, and linearmycin. For a DNJ gene cluster, homologs of gabT1 and gutB1 were commonly found; however, yktC1 was identified in only 112 genomes. The presence of several types of clusters suggests that different strains may produce different types of azasugars. Chemical-profile-inspired comparative genome analysis may facilitate a more accurate assessment of the biosynthetic potential to produce secondary metabolites.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Sign in / Sign up

Export Citation Format

Share Document