Interaction of quantitative trait loci for resistance to common bacterial blight and pathogen isolates in Phaseolus vulgaris L.

Breeding a model plant that encompasses individual traits thought to enhance yield potential, known as ideotype breeding, has traditionally focused on phenotypic selection of plants with desirable morphological traits. Broadening this breeding method to the molecular level through the use of molecular markers would avoid the environmental interactions associated with phenotypic selection. A population of 110 F5 recombinant inbred lines (RILs), derived from the cross between WO3391 and 'OAC Speedvale', was used to develop a genetic linkage map consisting of 105 random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and sequence-tagged site (STS) markers. The map has a total length of 641 cM distributed across 8 linkage groups (LGs). Five of them were aligned on the core linkage map of bean. Twenty-one quantitative trait loci (QTLs) were identified over three environments for eight agronomic and architectural traits previously defined for a bean (Phaseolus vulgaris L.) ideotype. The QTLs were mapped to seven LGs with several regions containing QTLs for multiple traits. At least one QTL was located for each trait and a maximum of four were associated with lodging. Total explained phenotypic variance ranged from 10.6% for hypocotyl diameter to 45.4% for maturity. Some of the QTLs identified will be useful for early generation selection of tall, upright, high-yielding lines in a breeding program.Key words: molecular markers, genetic map, plant architecture, yield.

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Quantitative Trait Loci Associated with Bacterial Brown Spot in Phaseolus vulgaris L.

Crop Science ◽

10.2135/cropsci2006.01.0056 ◽

2007 ◽

Vol 47 (4) ◽

pp. 1344-1353 ◽

Cited By ~ 9

Author(s):

Felix Navarro ◽

Paul Skroch ◽

Geunhwa Jung ◽

James Nienhuis

Keyword(s):

Quantitative Trait Loci ◽

Phaseolus Vulgaris ◽

Quantitative Trait ◽

Brown Spot ◽

Phaseolus Vulgaris L ◽

Trait Loci ◽

Bacterial Brown Spot

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Interaction of common bacterial blight quantitative trait loci in a resistant inter-cross population of common bean

Plant Breeding ◽

10.1111/pbr.12103 ◽

2013 ◽

Vol 132 (6) ◽

pp. 658-666 ◽

Cited By ~ 7

Author(s):

Kelli M. Durham ◽

Weilong Xie ◽

Kangfu Yu ◽

K. Peter Pauls ◽

Elizabeth Lee ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Common Bean ◽

Quantitative Trait ◽

Bacterial Blight ◽

Common Bacterial Blight ◽

Trait Loci

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Interaction of Common Bacterial Blight Bacteria with Disease Resistance Quantitative Trait Loci in Common Bean

Phytopathology ◽

10.1094/phyto-03-10-0095 ◽

2011 ◽

Vol 101 (4) ◽

pp. 425-435 ◽

Cited By ~ 13

Author(s):

Robert W. Duncan ◽

Shree P. Singh ◽

Robert L. Gilbertson

Keyword(s):

Quantitative Trait Loci ◽

Common Bean ◽

Quantitative Trait ◽

Bacterial Blight ◽

Brown Pigment ◽

Common Bacterial Blight ◽

Specific Primers ◽

Geographical Regions ◽

New Genotype ◽

Trait Loci

Common bacterial blight (CBB) of common bean (Phaseolus vulgaris L.) is caused by Xanthomonas campestris pv. phaseoli and X. fuscans subsp. fuscans, and is the most important bacterial disease of this crop in many regions of the world. In 2005 and 2006, dark red kidney bean fields in a major bean-growing region in central Wisconsin were surveyed for CBB incidence and representative symptomatic leaves collected. Xanthomonad-like bacteria were isolated from these leaves and characterized based upon phenotypic (colony) characteristics, pathogenicity on common bean, polymerase chain reaction (PCR) with X. campestris pv. phaseoli- and X. fuscans subsp. fuscans-specific primers, and repetitive-element PCR (rep-PCR) and 16S-28S ribosomal RNA spacer region sequence analyses. Of 348 isolates that were characterized, 293 were identified as common blight bacteria (i.e., pathogenic on common bean and positive in PCR tests with the X. campestris pv. phaseoli- and X. fuscans subsp. fuscans-specific primers), whereas the other isolates were nonpathogenic xanthomonads. Most (98%) of the pathogenic xanthomonads were X. campestris pv. phaseoli, consistent with the association of this bacterium with CBB in large-seeded bean cultivars of the Andean gene pool. Two types of X. campestris pv. phaseoli were involved with CBB in this region: typical X. campestris pv. phaseoli (P) isolates with yellow mucoid colonies, no brown pigment production, and a typical X. campestris pv. phaseoli rep-PCR fingerprint (60% of strains); and a new phenotype and genotype (Px) with an X. campestris pv. phaseoli-type fingerprint and less mucoid colonies that produced brown pigment (40% of strains). In addition, a small number of X. fuscans subsp. fuscans strains, representing a new genotype (FH), were isolated from two fields in 2005. Representative P and Px X. campestris pv. phaseoli strains, an FH X. fuscans subsp. fuscans strain, plus five previously characterized X. campestris pv. phaseoli and X. fuscans subsp. fuscans genotypes were inoculated onto 28 common bean genotypes having various combinations of known CBB resistance quantitative trait loci (QTL) and associated sequence-characterized amplified region markers. Different levels of virulence were observed for X. campestris pv. phaseoli strains, whereas X. fuscans subsp. fuscans strains were similar in virulence. The typical X. campestris pv. phaseoli strain from Wisconsin was most virulent, whereas X. campestris pv. phaseoli genotypes from East Africa were the least virulent. Host genotypes having the SU91 marker-associated resistance and one or more other QTL (i.e., pyramided resistance), such as the VAX lines, were highly resistant to all genotypes of common blight bacteria tested. This information will help in the development of CBB resistance-breeding strategies for different common bean market classes in different geographical regions, as well as the identification of appropriate pathogen genotypes for screening for resistance.

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Development of a Mesoamerican intra-genepool genetic map for quantitative trait loci detection in a drought tolerant × susceptible common bean (Phaseolus vulgaris L.) cross

Molecular Breeding ◽

10.1007/s11032-010-9527-9 ◽

2010 ◽

Vol 29 (1) ◽

pp. 71-88 ◽

Cited By ~ 57

Author(s):

Matthew W. Blair ◽

Carlos H. Galeano ◽

Eduardo Tovar ◽

Monica C. Muñoz Torres ◽

Alejandro Velasco Castrillón ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Phaseolus Vulgaris ◽

Common Bean ◽

Genetic Map ◽

Quantitative Trait ◽

Phaseolus Vulgaris L ◽

Drought Tolerant ◽

Trait Loci

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Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L.

Genome ◽

10.1139/g97-071 ◽

1997 ◽

Vol 40 (4) ◽

pp. 544-551 ◽

Cited By ~ 25

Author(s):

Yonghe Bai ◽

T. E. Michaels ◽

K. P. Pauls

Keyword(s):

Phaseolus Vulgaris ◽

Linkage Group ◽

Bacterial Blight ◽

Rapd Markers ◽

Interspecific Cross ◽

Breeding Population ◽

Common Bacterial Blight ◽

Linkage Groups ◽

Phaseolus Vulgaris L ◽

Total Contribution

Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.Key words: Phaseolus vulgaris L., common bacterial blight (CBB), polymerase chain reaction (PCR), RAPD markers, linkage groups.

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Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

Plant Health Progress ◽

10.1094/php-2013-0923-01-br ◽

2013 ◽

Vol 14 (1) ◽

pp. 41 ◽

Cited By ~ 1

Author(s):

Y. He ◽

G. P. Munkvold

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Bacterial Blight ◽

Disease Incidence ◽

Seed Transmission ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Transmission Frequency ◽

Xanthomonas Axonopodis ◽

Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

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Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.)

Canadian Journal of Plant Science ◽

10.4141/cjps-2014-399 ◽

2015 ◽

Vol 95 (4) ◽

pp. 703-710 ◽

Cited By ~ 4

Author(s):

J. G. Boersma ◽

A. Hou ◽

C. L. Gillard ◽

K. B. McRae ◽

R. L. Conner

Keyword(s):

Phaseolus Vulgaris ◽

Seed Weight ◽

Bacterial Blight ◽

Common Bacterial Blight ◽

Yield Losses ◽

Dry Beans ◽

Phaseolus Vulgaris L ◽

Navy Bean ◽

Navy Beans ◽

The Impact

Boersma, J. G., Hou, A., Gillard, C. L., McRae, K. B. and Conner, R. L. 2015. Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.). Can. J. Plant Sci. 95: 703–710. Common bacterial blight (CBB) is a seed-borne disease of dry bean (Phaseolus vulgaris L.), causing significant economic loss to growers due to reductions in seed yield and quality and the need to annually purchase disease-free seed. Over the past decade a number of breeding lines and cultivars with resistance to CBB have been developed in several bean market classes including navy, black and cranberry beans. A comparison of three susceptible navy bean cultivars and seven resistant navy, three black and one cranberry bean entries in Manitoba revealed that most resistant navy and black bean lines had significantly reduced the incidence of leaf symptoms and their mean yield losses were reduced to less than 17%, while those of the susceptible lines were as high as 36% under severe disease pressure. Only the weakly resistant navy bean cultivar HR67 and the cranberry bean line F4GR1 failed to substantially reduce CBB symptoms or show a yield advantage. The Simple Sequence Repeat (SSR) marker PVctt001 in combination with Sequence Characterized Amplified Region (SCAR) marker SU91 was associated with a low incidence of CBB symptoms and a reduced yield loss in five navy bean lines, but not in the cranberry bean line F4GR1. Disease symptoms on the pods in the resistant black and navy beans and seed discoloration of navy beans caused by CBB were also significantly reduced by resistance. Seed weights were reduced by 2.1–4.7% in the susceptible cultivars, but there was only a slight or no decrease or no effect on the seed weight of the CBB-resistant lines and cultivars. Generally the magnitude of the reductions in yield was much greater than the impact on seed weight, which suggests that yield losses were caused by a combination of reduced seed weight and the number of seeds per plant.

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Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽

10.1139/g01-099 ◽

2001 ◽

Vol 44 (6) ◽

pp. 1046-1056 ◽

Cited By ~ 19

Author(s):

B Tar'an ◽

T E Michaels ◽

K P Pauls

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Length Polymorphism ◽

Bacterial Blight ◽

Genetic Factors ◽

Fragment Length Polymorphism ◽

Segregation Ratio ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

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