Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L.

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 544-551 ◽  
Author(s):  
Yonghe Bai ◽  
T. E. Michaels ◽  
K. P. Pauls
Keyword(s):  
Phaseolus Vulgaris ◽  
Linkage Group ◽  
Bacterial Blight ◽  
Rapd Markers ◽  
Interspecific Cross ◽  
Breeding Population ◽  
Common Bacterial Blight ◽  
Linkage Groups ◽  
Phaseolus Vulgaris L ◽  
Total Contribution

Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.Key words: Phaseolus vulgaris L., common bacterial blight (CBB), polymerase chain reaction (PCR), RAPD markers, linkage groups.

Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

2013 ◽  
Vol 14 (1) ◽  
pp. 41 ◽  
Author(s):  
Y. He ◽  
G. P. Munkvold
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Bacterial Blight ◽  
Disease Incidence ◽  
Seed Transmission ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Transmission Frequency ◽  
Xanthomonas Axonopodis ◽  
Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

scholarly journals Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.)

2015 ◽  
Vol 95 (4) ◽  
pp. 703-710 ◽  
Author(s):  
J. G. Boersma ◽  
A. Hou ◽  
C. L. Gillard ◽  
K. B. McRae ◽  
R. L. Conner
Keyword(s):  
Phaseolus Vulgaris ◽  
Seed Weight ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Yield Losses ◽  
Dry Beans ◽  
Phaseolus Vulgaris L ◽  
Navy Bean ◽  
Navy Beans ◽  
The Impact

Boersma, J. G., Hou, A., Gillard, C. L., McRae, K. B. and Conner, R. L. 2015. Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.). Can. J. Plant Sci. 95: 703–710. Common bacterial blight (CBB) is a seed-borne disease of dry bean (Phaseolus vulgaris L.), causing significant economic loss to growers due to reductions in seed yield and quality and the need to annually purchase disease-free seed. Over the past decade a number of breeding lines and cultivars with resistance to CBB have been developed in several bean market classes including navy, black and cranberry beans. A comparison of three susceptible navy bean cultivars and seven resistant navy, three black and one cranberry bean entries in Manitoba revealed that most resistant navy and black bean lines had significantly reduced the incidence of leaf symptoms and their mean yield losses were reduced to less than 17%, while those of the susceptible lines were as high as 36% under severe disease pressure. Only the weakly resistant navy bean cultivar HR67 and the cranberry bean line F4GR1 failed to substantially reduce CBB symptoms or show a yield advantage. The Simple Sequence Repeat (SSR) marker PVctt001 in combination with Sequence Characterized Amplified Region (SCAR) marker SU91 was associated with a low incidence of CBB symptoms and a reduced yield loss in five navy bean lines, but not in the cranberry bean line F4GR1. Disease symptoms on the pods in the resistant black and navy beans and seed discoloration of navy beans caused by CBB were also significantly reduced by resistance. Seed weights were reduced by 2.1–4.7% in the susceptible cultivars, but there was only a slight or no decrease or no effect on the seed weight of the CBB-resistant lines and cultivars. Generally the magnitude of the reductions in yield was much greater than the impact on seed weight, which suggests that yield losses were caused by a combination of reduced seed weight and the number of seeds per plant.

Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1046-1056 ◽  
Author(s):  
B Tar'an ◽  
T E Michaels ◽  
K P Pauls
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Length Polymorphism ◽  
Bacterial Blight ◽  
Genetic Factors ◽  
Fragment Length Polymorphism ◽  
Segregation Ratio ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

scholarly journals Diversity and interaction of common bacterial blight disease-causing bacteria (Xanthomonas spp.) with Phaseolus vulgaris L.

2019 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
Joab K. Tugume ◽  
Geoffrey Tusiime ◽  
Allan Male Sekamate ◽  
Robin Buruchara ◽  
Clare Mugisha Mukankusi
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Bacterial Blight Disease ◽  
Blight Disease

scholarly journals REPOSIÇÕES DE ÁGUA NO SOLO E INCIDÊNCIA DE DOENÇAS FOLIARES EM CULTIVARES DE FEIJOEIRO (Phaseolus vulgaris L.)

Irriga ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 697-712
Author(s):  
Taynara Tuany Borges Valeriano ◽  
Ana Flávia Oliveira ◽  
Márcio José Santana ◽  
Isaias Antônio de Paiva ◽  
Daniel Rufino Amaral
Keyword(s):  
Phaseolus Vulgaris ◽  
Soil Water ◽  
Bacterial Blight ◽  
Irrigation Management ◽  
Common Bacterial Blight ◽  
Crop Evapotranspiration ◽  
Phaseolus Vulgaris L ◽  
Foliar Diseases ◽  
Bean Crop ◽  
Water Depths

 REPOSIÇÕES DE ÁGUA NO SOLO E INCIDÊNCIA DE DOENÇAS FOLIARES EM CULTIVARES DE FEIJOEIRO (Phaseolus vulgaris L.)     TAYNARA TUANY BORGES VALERIANO1; ANA FLÁVIA OLIVEIRA2; MÁRCIO JOSÉ DE SANTANA3; ISAÍAS ANTONIO DE PAIVA4 E DANIEL RUFINO AMARAL5   1 Departamento de Ciências Exatas, Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castelane, S/N, Vila Industrial – CEP: 14884-900, Jaboticabal-SP, Brasil, [email protected]; 2 Instituto Federal de Educação Ciência e Tecnologia do Triângulo Mineiro, Rua João Batista Ribeiro, 4000, Distrito Industrial II - CEP: 38064-790, Uberaba-MG, Brasil, [email protected]; 3 Instituto Federal de Educação Ciência e Tecnologia do Triângulo Mineiro, Rua João Batista Ribeiro, 4000, Distrito Industrial II - CEP: 38064-790, Uberaba-MG, Brasil, [email protected]; 4 Departamento de Solos e Engenharia Agrícola, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Juvevê - CEP:80035-050, Curitiba-PR, Brasil, [email protected]; 5 Instituto Federal de Educação Ciência e Tecnologia do Triângulo Mineiro, Rua João Batista Ribeiro, 4000 - Bairro: Distrito Industrial II - CEP: 38064-790 – Uberaba-MG, [email protected].     1 RESUMO   A incidência de doenças foliares é um dos principais fatores que interferem na produtividade, e está diretamente relacionada com as lâminas de água aplicadas na cultura do feijoeiro. Objetivou-se com este trabalho avaliar o efeito das reposições de água no solo sobre a incidência de doenças foliares em cultivares de feijoeiro comum (Phaseolus vulgaris L.). O experimento foi conduzido em uma casa de vegetação, localizada no Instituto Federal de Educação, Ciência e Tecnologia do Triângulo Mineiro (IFTM Campus - Uberaba). O delineamento experimental foi em blocos casualizados com cinco repetições, disposto em um esquema fatorial 2x5, sendo duas cultivares: IAC Imperador e IPR Juriti, cinco reposições de água no solo, 40%, 60%, 80%, 100% e 120% da evapotranspiração da cultura (ETc), e cinco repetições. As doenças avaliadas foram crestamento bacteriano e mancha de alternaria, no intervalo de 10 dias. O manejo da irrigação a 100% e 120% de ETc registraram maior severidade de mancha de alternaria e crestamento bacteriano comum para cultivar IPR Juriti. Houve um aumento linear e quadrático da AACPD para crestamento bacteriano comum e mancha de alternaria, respectivamente, com aumento das lâminas de irrigação. As maiores médias de produtividade do feijoeiro, assim como a maior eficiência do uso da água (EUA) foram obtidas com a lâmina de 80% da ETc.   Palavras-chave: fitopatologia, crestamento bacteriano, mancha de alternaria, evapotranspiração.     VALERIANO, T. T. B.; OLIVEIRA, A. F.; SANTANA, M. J. de; PAIVA, I. A., AMARAL, D. R. WATER REPLENISHMENT ON SOIL AND IMPACT OF FOLIARY DISEASES ON FEIJOEIRO CULTIVARS (Phaseolus vulgaris L.)         2 ABSTRACT   The incidence of foliar diseases is one of the main factors that interfere with yield and is directly related to the water depths applied in bean crop. The objective of this work was to evaluate the effect of soil water replenishment on foliar diseases incommon bean cultivars. The experiment was conducted in a greenhouse located at the Instituto Federal do Triângulo Mineiro (IFTM Campus - Uberaba). The experimental design was randomized blocks with five replications, arranged in a 2x5 factorial arrangement, two cultivars, IAC Imperador and IPR Juriti, five replenishments of soil water, 40%, 60%, 80%, 100% and 120% of crop evapotranspiration, and five water replacements. Diseases evaluated were common bacterial blight and alternaria spot in the 10-day interval. The irrigation management was carried out by the method of percolating water collection drains. Depth corresponding to 100% and 120% ETC showed higher severity of alternaria and common bacterial blight to cultivar IPR Juriti. There was linear and quadratic increase of ASCPD for common bacterial blight and alternaria spot, respectively, with increased depth. The highest productivity values of common bean, as well as greater efficiency in water use, were obtained with depth of approximately 80% of the ETC.   Keywords: plant pathology, bacterial blight, alternaria spot, evapotranspiration.

AC Scarlet dry bean

2001 ◽  
Vol 81 (4) ◽  
pp. 731-732
Author(s):  
Hans-Henning Mündel ◽  
Ferdinand A. Kiehn ◽  
Gilles Saindon ◽  
Henry C. Huang
Keyword(s):  
Phaseolus Vulgaris ◽  
Key Words ◽  
Bacterial Blight ◽  
White Mold ◽  
High Yield ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Canadian Prairies ◽  
Dry Bean ◽  
Red Bean

AC Scarlet is a high-yielding small red dry bean (Phaseolus vulgaris L.) cultivar. AC Scarlet is well adapted to the eastern Canadian prairies and yielded significantly more than the check cultivar NW 63. AC Scarlet is susceptible to white mold and susceptible to common bacterial blight. Key words: Phaseolus vulgaris, small red bean, high yield

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