Strain Enterococcus faecalis BGPT1-10P was isolated from artisanal semi-hard
homemade cheese from Stara Planina, Serbia. Results showed that BGPT1-10P
synthesized a heat labile bacteriocin with a broad spectrum of activity,
including Listeria and Candida species. Further analysis revealed that
synthesized bacteriocin is enterolysin A. Moreover, the entL gene encoding
enterolysin A was found to be located on the chromosome. The entL gene was
cloned and sequenced. Analysis of nucleotide sequence showed that the entL
gene in natural isolate En. faecalis BGPT1-10P is identical to that of the
entL gene described previously in En. faecalis LMG 2333. Within the cloned
DNA fragment containing the entL gene, four ORFs were detected. One of them
was identified as the scpE gene, which encodes a virulent factor staphopain
peptidase. Functional analysis of the entL gene showed that the complete
genetic information necessary for the synthesis of enterolysin A were
directly linked solely to it. Strain BGPT1-10P also synthesized gelatinase
and citolysin, and contained a set of virulent factors. In addition,
BGPT1-10P carries the ermB and tetM genes conferring the resistance to
erythromycin and tetracycline, respectively.
Molecular analysis of enterolysin a and entL gene cluster from natural isolate Enterococcus faecalis BGPT1-10P
