Functional analysis of the promoter of the MdFRK2 gene encoding a high-affinity fructokinase in apple (malus × domestica)

2020 ◽  
Vol 265 ◽  
pp. 109088
Author(s):  
Jingjing Yang ◽  
Ruiling Zhan ◽  
Yuru Jin ◽  
JianYu Song ◽  
Dongxia Li ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Malus Domestica ◽  
Gene Encoding ◽  
High Affinity

Isolation and functional analysis of MdCS1: a gene encoding a citrate synthase in Malus domestica (L.) Borkh

2014 ◽  
Vol 75 (1) ◽  
pp. 209-218 ◽  
Author(s):  
Deguo Han ◽  
Yan Shi ◽  
Zeyuan Yu ◽  
Wei Liu ◽  
Bingyu Lv ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Malus Domestica ◽  
Citrate Synthase ◽  
Gene Encoding

scholarly journals Molecular analysis of enterolysin a and entL gene cluster from natural isolate Enterococcus faecalis BGPT1-10P

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 479-492 ◽  
Author(s):  
Katarina Veljovic ◽  
Amarela Terzic-Vidojevic ◽  
Maja Tolinacki ◽  
Milan Kojic ◽  
Ljubisa Topisirovic
Keyword(s):  
Functional Analysis ◽  
Nucleotide Sequence ◽  
Enterococcus Faecalis ◽  
Broad Spectrum ◽  
Candida Species ◽  
Genetic Information ◽  
Natural Isolate ◽  
Gene Encoding ◽  
Dna Fragment ◽  
Virulent Factor

Strain Enterococcus faecalis BGPT1-10P was isolated from artisanal semi-hard homemade cheese from Stara Planina, Serbia. Results showed that BGPT1-10P synthesized a heat labile bacteriocin with a broad spectrum of activity, including Listeria and Candida species. Further analysis revealed that synthesized bacteriocin is enterolysin A. Moreover, the entL gene encoding enterolysin A was found to be located on the chromosome. The entL gene was cloned and sequenced. Analysis of nucleotide sequence showed that the entL gene in natural isolate En. faecalis BGPT1-10P is identical to that of the entL gene described previously in En. faecalis LMG 2333. Within the cloned DNA fragment containing the entL gene, four ORFs were detected. One of them was identified as the scpE gene, which encodes a virulent factor staphopain peptidase. Functional analysis of the entL gene showed that the complete genetic information necessary for the synthesis of enterolysin A were directly linked solely to it. Strain BGPT1-10P also synthesized gelatinase and citolysin, and contained a set of virulent factors. In addition, BGPT1-10P carries the ermB and tetM genes conferring the resistance to erythromycin and tetracycline, respectively.

scholarly journals Functional analysis of the aroC gene encoding chorismate synthase from Xanthomonas oryzae pathovar oryzae

2012 ◽  
Vol 167 (6) ◽  
pp. 326-331 ◽  
Author(s):  
Eun-Sung Song ◽  
Young-Jin Park ◽  
Tae-Hwan Noh ◽  
Yeong-Tae Kim ◽  
Jeong-Gu Kim ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Xanthomonas Oryzae ◽  
Gene Encoding ◽  
Chorismate Synthase

scholarly journals Monosomy-3 Alters the Expression Profile of the Glucose Transporters GLUT1-3 in Uveal Melanoma

2020 ◽  
Vol 21 (24) ◽  
pp. 9345
Author(s):  
Tjorge Maaßen ◽  
Siranush Vardanyan ◽  
Anton Brosig ◽  
Hartmut Merz ◽  
Mahdy Ranjbar ◽  
...  
Keyword(s):  
Uveal Melanoma ◽  
Glucose Transporter ◽  
Glucose Transporters ◽  
Clinical Factors ◽  
Gene Encoding ◽  
Primary Tumors ◽  
High Affinity ◽  
Monosomy 3 ◽  
Glucose Transporter Glut1 ◽  
Transporter Glut1

Monosomy-3 in uveal melanoma (UM) cells increases the risk of fatal metastases. The gene encoding the low-affinity glucose transporter GLUT2 resides on chromosome 3q26.2. Here, we analyzed the expression of the glucose transporters GLUT1, GLUT2, and GLUT3 with regard to the histological and clinical factors by performing immunohistochemistry on the primary tumors of n = 33 UM patients. UMs with monosomy-3 exhibited a 57% lower immunoreactivity for GLUT2 and a 1.8×-fold higher ratio of GLUT1 to total GLUT1-3. The combined levels of GLUT1-3 proteins were reduced in the irradiated but not the non-irradiated tumors with monosomy-3. GLUT3 expression was stronger in the irradiated samples with disomy-3 versus monosomy-3, but the ratio of the GLUT3 isoform to total GLUT1-3 did not differ with regard to the monosomy-3 status in the irradiated or non-irradiated subgroups. Systemic metastases were associated with the presence of monosomy-3 in the primary and circulating tumor cells as well as a higher GLUT1 ratio. Upregulation of the high-affinity glucose transporter GLUT1 possibly as a compensation for the low-affinity isoform GLUT2 may be enhancing the basal glucose uptake in the UM cells with monosomy-3. Prevention of hyperglycemia might, therefore, be a valuable approach to delay the lethal UM metastases.

scholarly journals Functional analysis of a gene encoding threonine synthase from rice

2010 ◽  
Vol 9 (8) ◽  
pp. 1122-1129 ◽  
Author(s):  
Shafiqul Islam Sikdar Md ◽  
Kim Jung Sup
Keyword(s):  
Functional Analysis ◽  
Gene Encoding ◽  
Threonine Synthase
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