scholarly journals Molecular analysis of enterolysin a and entL gene cluster from natural isolate Enterococcus faecalis BGPT1-10P

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 479-492 ◽  
Author(s):  
Katarina Veljovic ◽  
Amarela Terzic-Vidojevic ◽  
Maja Tolinacki ◽  
Milan Kojic ◽  
Ljubisa Topisirovic
Keyword(s):  
Functional Analysis ◽  
Nucleotide Sequence ◽  
Enterococcus Faecalis ◽  
Broad Spectrum ◽  
Candida Species ◽  
Genetic Information ◽  
Natural Isolate ◽  
Gene Encoding ◽  
Dna Fragment ◽  
Virulent Factor

Strain Enterococcus faecalis BGPT1-10P was isolated from artisanal semi-hard homemade cheese from Stara Planina, Serbia. Results showed that BGPT1-10P synthesized a heat labile bacteriocin with a broad spectrum of activity, including Listeria and Candida species. Further analysis revealed that synthesized bacteriocin is enterolysin A. Moreover, the entL gene encoding enterolysin A was found to be located on the chromosome. The entL gene was cloned and sequenced. Analysis of nucleotide sequence showed that the entL gene in natural isolate En. faecalis BGPT1-10P is identical to that of the entL gene described previously in En. faecalis LMG 2333. Within the cloned DNA fragment containing the entL gene, four ORFs were detected. One of them was identified as the scpE gene, which encodes a virulent factor staphopain peptidase. Functional analysis of the entL gene showed that the complete genetic information necessary for the synthesis of enterolysin A were directly linked solely to it. Strain BGPT1-10P also synthesized gelatinase and citolysin, and contained a set of virulent factors. In addition, BGPT1-10P carries the ermB and tetM genes conferring the resistance to erythromycin and tetracycline, respectively.

Molecular cloning and nucleotide sequence analysis of the gene encoding the immunoreactive Brucella abortus Hsp60 protein, BA60K

1992 ◽  
Vol 12 (1) ◽  
pp. 47-62 ◽  
Author(s):  
R.Martin Roop ◽  
Michelle L. Price ◽  
Bruce E. Dunn ◽  
Stephen M. Boyle ◽  
Nammalwar Sriranganathan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Molecular Cloning ◽  
Brucella Abortus ◽  
Nucleotide Sequence Analysis ◽  
Gene Encoding ◽  
Hsp60 Protein

Nucleotide sequence of the Clostridium thermocellum bglB gene encoding thermostable β-glucosidase B: Homology to fungal β-glucosidases

1989 ◽  
Vol 217 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Folke Gräbnitz ◽  
Karl P. Rücknagel ◽  
Monika Seiß ◽  
Walter L. Staudenhauer
Keyword(s):  
Nucleotide Sequence ◽  
Clostridium Thermocellum ◽  
Gene Encoding

scholarly journals Broad-Spectrum Biosensor Capable of Detecting and Identifying Diverse Bacterial and Candida Species in Blood

2013 ◽  
Vol 51 (8) ◽  
pp. 2670-2678 ◽  
Author(s):  
D. Metzgar ◽  
M. Frinder ◽  
R. Lovari ◽  
D. Toleno ◽  
C. Massire ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Candida Species

scholarly journals Molecular Characterization of the Gene Encoding a New AmpC β-Lactamase in a Clinical Strain of Acinetobacter Genomic Species 3

2004 ◽  
Vol 48 (4) ◽  
pp. 1374-1378 ◽  
Author(s):  
Alejandro Beceiro ◽  
Lourdes Dominguez ◽  
Anna Ribera ◽  
Jordi Vila ◽  
Francisca Molina ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Biochemical Properties ◽  
Clinical Strain ◽  
The Novel ◽  
Gene Encoding ◽  
Genomic Species ◽  
First Time

ABSTRACT A presumptive chromosomal cephalosporinase (pI, 9.0) from a clinical strain of Acinetobacter genomic species 3 (AG3) is reported. The nucleotide sequence of this β-lactamase shows for the first time the gene encoding an AmpC enzyme in AG3. In addition, the biochemical properties of the novel AG3 AmpC β-lactamase are reported

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