scholarly journals Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda

2010 ◽  
Vol 38 (1) ◽  
pp. 177-182 ◽  
Author(s):  
Tran Thanh ◽  
Vu Thi Quynh Chi ◽  
Mohd Puad Abdullah ◽  
Hishamuddin Omar ◽  
Mostafa Noroozi ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Preliminary Analysis ◽  
Ankistrodesmus Convolutus ◽  
Green Microalga ◽  
Expressed Sequence

Construction of a cDNA library and preliminary analysis of expressed sequence tags in Piper hainanense

2015 ◽  
Vol 14 (4) ◽  
pp. 12733-12745 ◽  
Author(s):  
R. Fan ◽  
P. Ling ◽  
C.Y. Hao ◽  
F.P. Li ◽  
L.F. Huang ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Preliminary Analysis ◽  
Expressed Sequence

Construction of a cDNA Library from the Ephemeral Plant Olimarabidopsis pumila and Preliminary Analysis of Expressed Sequence Tags

2013 ◽  
Vol 68 (11-12) ◽  
pp. 499-508 ◽  
Author(s):  
Yun-Xia Zhao ◽  
Yan-Ling Wei ◽  
Ping Zhao ◽  
Cheng-Bin Xiang ◽  
Fang Xu ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Preliminary Analysis ◽  
Restriction Enzymes ◽  
Close Relative ◽  
Ephemeral Plant ◽  
Young Leaves ◽  
Model Plant Arabidopsis Thaliana ◽  
Salt Tolerance Gene ◽  
Expressed Sequence

Olimarabidopsis pumila is a close relative of the model plant Arabidopsis thaliana but, unlike A. thaliana, it is a salt-tolerant ephemeral plant that is widely distributed in semi-arid and semi-salinized regions of the Xinjiang region of China, thus providing an ideal candidate plant system for salt tolerance gene mining. A good-quality cDNA library was constructed using cap antibody to enrich full-length cDNA with the gateway technology allowing library construction without traditional methods of cloning by use of restriction enzymes. A preliminary analysis of expressed sequence tags (ESTs) was carried out. The titers of the primary and the normalized cDNA library were 1.6 · 106 cfu/mL and 6.7 · 106 cfu/mL, respectively. A total of 1093 clones were randomly selected from the normalized library for EST sequencing. By sequence analysis, 894 high-quality ESTs were generated and assembled into 736 unique sequences consisting of 72 contigs and 664 singletons. The resulting unigenes were categorized according to the gene ontology (GO) hierarchy. The potential roles of gene products associated with stress-related ESTs are discussed. The 736 unigenes were similar to A. thaliana, A. lyrata, or Thellungiella salsuginea. This research provides an overview of the mRNA expression profile and first-hand information of gene sequence expressed in young leaves of O. pumila.

Construction of cDNA library and preliminary analysis of expressed sequence tags from tea plant [Camellia sinensis (L) O. Kuntze]

Gene ◽  
2012 ◽  
Vol 506 (1) ◽  
pp. 202-206 ◽  
Author(s):  
Munmi Phukon ◽  
Richa Namdev ◽  
Diganta Deka ◽  
Mahendra K. Modi ◽  
Priyabrata Sen
Keyword(s):  
Cdna Library ◽  
Camellia Sinensis ◽  
Expressed Sequence Tags ◽  
Preliminary Analysis ◽  
Tea Plant ◽  
Expressed Sequence

scholarly journals Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger

2010 ◽  
pp. 584-589 ◽  
Author(s):  
Chang-Qing Liu ◽  
Tao-Feng Lu ◽  
Bao-Gang Feng ◽  
Dan Liu ◽  
Wei-Jun Guan ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Preliminary Analysis ◽  
Siberian Tiger ◽  
Expressed Sequence
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