Construction of cDNA library and preliminary analysis of expressed sequence tags from tea plant [Camellia sinensis (L) O. Kuntze]

AbstractThe construction of two cDNA libraries from young shoots of tea plant [Camellia sinensis cv. Longjing 43 and Anji Baicha], the sequencing of Longjing 43 cDNA clones and the analysis of expressed sequence tags (ESTs) are reported. Totally, 4320 clones from the cDNA library of Longjing 43 were sequenced, and 2963 useful sequences were obtained, corresponding to 68.6% of clones. A total of 416 clones shorter than 150 bp and 863 repeated clones were excluded, the first 1684 valid tea plant ESTs were generated. Most of the ESTs were between 300 and 700 bp, with an average of 478 bp. Six hundred and seven ESTs with known function or putative function were identified by BlastN searches against the National Center of Biotechnology Institute (NCBI) non-redundant nucleotide databases, corresponding to more than 130 functional genes in the tea plant. The rest, 1077 ESTs, were novel gene partial or full sequences. The results indicated that EST sequencing was a rapid and effective approach to identification of novel functional genes for tea plant.

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Construction of a cDNA library and preliminary analysis of expressed sequence tags in Piper hainanense

Genetics and Molecular Research ◽

10.4238/2015.october.19.17 ◽

2015 ◽

Vol 14 (4) ◽

pp. 12733-12745 ◽

Cited By ~ 1

Author(s):

R. Fan ◽

P. Ling ◽

C.Y. Hao ◽

F.P. Li ◽

L.F. Huang ◽

...

Keyword(s):

Cdna Library ◽

Expressed Sequence Tags ◽

Preliminary Analysis ◽

Expressed Sequence

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Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes

Genetics and Molecular Research ◽

10.4238/2015.october.21.13 ◽

2015 ◽

Vol 14 (4) ◽

pp. 12921-12930

Author(s):

Y.H. Zhao ◽

Z.B. Zhang ◽

C.Q. Zhao ◽

Y. Zhang ◽

Y.F. Wang ◽

...

Keyword(s):

Cdna Library ◽

Expressed Sequence Tags ◽

Preliminary Analysis ◽

Full Length ◽

Full Length Cdna ◽

Expressed Sequence

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Construction of a cDNA Library from the Ephemeral Plant Olimarabidopsis pumila and Preliminary Analysis of Expressed Sequence Tags

Zeitschrift für Naturforschung C ◽

10.1515/znc-2013-11-1209 ◽

2013 ◽

Vol 68 (11-12) ◽

pp. 499-508 ◽

Cited By ~ 1

Author(s):

Yun-Xia Zhao ◽

Yan-Ling Wei ◽

Ping Zhao ◽

Cheng-Bin Xiang ◽

Fang Xu ◽

...

Keyword(s):

Cdna Library ◽

Expressed Sequence Tags ◽

Preliminary Analysis ◽

Restriction Enzymes ◽

Close Relative ◽

Ephemeral Plant ◽

Young Leaves ◽

Model Plant Arabidopsis Thaliana ◽

Salt Tolerance Gene ◽

Expressed Sequence

Olimarabidopsis pumila is a close relative of the model plant Arabidopsis thaliana but, unlike A. thaliana, it is a salt-tolerant ephemeral plant that is widely distributed in semi-arid and semi-salinized regions of the Xinjiang region of China, thus providing an ideal candidate plant system for salt tolerance gene mining. A good-quality cDNA library was constructed using cap antibody to enrich full-length cDNA with the gateway technology allowing library construction without traditional methods of cloning by use of restriction enzymes. A preliminary analysis of expressed sequence tags (ESTs) was carried out. The titers of the primary and the normalized cDNA library were 1.6 · 106 cfu/mL and 6.7 · 106 cfu/mL, respectively. A total of 1093 clones were randomly selected from the normalized library for EST sequencing. By sequence analysis, 894 high-quality ESTs were generated and assembled into 736 unique sequences consisting of 72 contigs and 664 singletons. The resulting unigenes were categorized according to the gene ontology (GO) hierarchy. The potential roles of gene products associated with stress-related ESTs are discussed. The 736 unigenes were similar to A. thaliana, A. lyrata, or Thellungiella salsuginea. This research provides an overview of the mRNA expression profile and first-hand information of gene sequence expressed in young leaves of O. pumila.

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