Selection of reference genes for quantitative real-time RT-PCR analysis in citrus

2011 ◽  
Vol 39 (2) ◽  
pp. 1831-1838 ◽  
Author(s):  
Jiawen Yan ◽  
Feirong Yuan ◽  
Guiyou Long ◽  
Lei Qin ◽  
Ziniu Deng
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Selection Of

Selection of Stable Reference Genes for Real-Time Quantitative PCR Analysis in Edwardsiella tarda

2017 ◽  
Vol 27 (1) ◽  
pp. 112-121 ◽  
Author(s):  
Zhongyang Sun ◽  
Jia Deng ◽  
Haizhen Wu ◽  
Qiyao Wang ◽  
Yuanxing Zhang
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reference Genes ◽  
Edwardsiella Tarda ◽  
Pcr Analysis ◽  
Real Time Quantitative Pcr ◽  
Selection Of

Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida

2018 ◽  
Vol 31 (1) ◽  
pp. 787-793 ◽  
Author(s):  
Jing Li ◽  
Haishu Huang ◽  
Tifeng Shan ◽  
Shaojun Pang
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Brown Alga ◽  
Undaria Pinnatifida ◽  
Rt Pcr ◽  
Selection Of

Characterization and Selection of Reference Genes for Real-time Quantitative RT-PCR of Plants

2013 ◽  
Vol 47 (4) ◽  
pp. 427-436 ◽  
Author(s):  
Yuan Wei ◽  
Wan Hongjian ◽  
Yang Yuejian
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Rt Pcr ◽  
Selection Of

scholarly journals Selection and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR Analysis in Needles of Larix olgensis under Abiotic Stresses

Forests ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 193
Author(s):  
Dandan Li ◽  
Sen Yu ◽  
Minzhen Zeng ◽  
Xiao Liu ◽  
Jia Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Real Time ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Stable Gene ◽  
Larix Olgensis ◽  
Qrt Pcr ◽  
Study Gene Expression ◽  
Selection Of

Larix olgensis Henry is an important afforestation species in northeastern China because of its fast juvenile growth, high-quality timber, and significant economic and ecological values. The selection of appropriate reference genes is necessary for the normalization of gene expression determination during quantitative real-time polymerase chain reaction (qRT-PCR) experiments. In this study, qRT-PCR was used to study gene expression. Three software packages geNorm, NormFinder, BestKeeper were used, and a comprehensive ranking of candidate reference genes was produced based on their output to evaluate the expression stability of 16 candidate reference genes from L. olgensis under drought, salt, cold, and heat stress. PP2A-1 and GAPDH ranked as the most stable reference genes under drought and cold stress, PP2A-1 and UBQ10 were most stable under salt stress, and TIP41 and ACT2 were most stable under heat stress. The least stable gene was ADP, which ranked the last under all treatments. Expression profile analysis of the antioxidant gene CAT using the two most stable and the single least stable reference genes under each stress further verified that the selected reference genes were suitable for gene expression normalization. This study provides an important foundation for the selection of suitable reference genes for the normalization and quantification of L. olgensis gene expression under abiotic stress conditions.

scholarly journals Selection of reliable reference genes for quantitative real-time RT-PCR in alfalfa

2015 ◽  
Vol 90 (3) ◽  
pp. 175-180 ◽  
Author(s):  
Xuemin Wang ◽  
Yuanyuan Fu ◽  
Liping Ban ◽  
Zan Wang ◽  
Guangyan Feng ◽  
...  
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Rt Pcr ◽  
Selection Of
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