scholarly journals Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cells

2021 ◽  
Author(s):  
Shiva Pratap Singh ◽  
Suresh Dinkar Kharche ◽  
Manisha Pathak ◽  
Ravi Ranjan ◽  
Yogesh Kumar Soni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oxygen Tension ◽  
Germline Stem Cells ◽  
Multilineage Differentiation ◽  
Low Oxygen ◽  
Male Germline ◽  
Low Oxygen Tension ◽  
Male Germline Stem Cells

A brief very-low oxygen tension regimen is sufficient for the early chondrogenic commitment of human adipose-derived mesenchymal stem cells

2021 ◽  
Vol 66 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Marco Govoni ◽  
Claudio Muscari ◽  
Francesca Bonafè ◽  
Paolo Giovanni Morselli ◽  
Marilisa Cortesi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Oxygen Tension ◽  
Low Oxygen ◽  
Low Oxygen Tension

scholarly journals Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Yinshan Bai ◽  
Cui Zhu ◽  
Meiying Feng ◽  
Hengxi Wei ◽  
Li Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Leydig Cells ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Male Germline Stem Cells ◽  
Porcine Testis

scholarly journals Low Oxygen Tension Potentiates Proliferation and Stemness but Not Multilineage Differentiation of Caprine Male Germline Stem Cells

2021 ◽  
Author(s):  
Shiva Pratap Singh ◽  
Suresh Dinkar Kharche ◽  
Manisha Pathak ◽  
Ravi Ranjan ◽  
Yogesh Kumar Soni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Growth Characteristics ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Germline Stem Cells ◽  
Multilineage Differentiation ◽  
Low Oxygen ◽  
Differentiation Potential

Abstract The milieu of testicular germline stem cells (mGSCs) is characterized as low oxygen (O2) environment, whereas, there in-vitro expansion is typically performed under normoxia (20-21% O2). Here, we evaluated and compared the culture and multilineage differentiation characteristics of enriched (through differential platting and percoll density centrifugation) caprine mGSCs (cmGSCs) under hypoxic (5% O2) and normoxic (21% O2) culture conditions. For this, in addition to growth characteristics and population-doubling time (PDT); viability, proliferation, senescence, and expression of key-markers of adhesion (β-integrin and E-Cadherin) and stemness (OCT-4, THY-1 and UCHL-1) were evaluated and compared under normoxia and hypoxia. Moreover, the extent of multilineage differentiation (neurogenic, adipogenic, and chondrogenic differentiation) was assessed. The survival, viability and proliferation were significantly promoted and PDT was reduced (p < 0.05), thus yielding a higher number of viable cells with larger colonies under hypoxia. Furthermore, expression of stemness and adhesion markers was distinctly increased under lowered O2 condition. Conversely, the presence of differentiated regions and expression of differentiation specific key genes [C/EBPα (adipogenic), nestin and β-tubulin (neurogenic), and COL2A1 (chondrogenic)] were significantly (p < 0.05) reduced under hypoxic conditions. These data demonstrate that culturing cmGSCs under hypoxia augments the growth characteristics, and stemness but not the multilineage differentiation potential of cmGSCs as compared with normoxia. These data are important for the development of robust methodologies for ex-vivo expansion and lineage-committed differentiation of cmGSCs for clinical applications.

scholarly journals High telomerase is a hallmark of undifferentiated spermatogonia and is required for maintenance of male germline stem cells

2015 ◽  
Vol 29 (23) ◽  
pp. 2420-2434 ◽  
Author(s):  
Matthew F. Pech ◽  
Alina Garbuzov ◽  
Kazuteru Hasegawa ◽  
Meena Sukhwani ◽  
Ruixuan J. Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Male Germline Stem Cells

scholarly journals Functional Assessment of Self-Renewal Activity of Male Germline Stem Cells Following Cytotoxic Damage and Serial Transplantation1

2003 ◽  
Vol 68 (5) ◽  
pp. 1801-1807 ◽  
Author(s):  
Mito Kanatsu-Shinohara ◽  
Shinya Toyokuni ◽  
Takeshi Morimoto ◽  
Shigeyuki Matsui ◽  
Tasuku Honjo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Assessment ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Male Germline ◽  
Male Germline Stem Cells
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