Synovial membrane mesenchymal stem cells for cartilaginous tissues repair

2022 ◽  
Author(s):  
Yessica Zamudio-Cuevas ◽  
Ricardo Plata-Rodríguez ◽  
Javier Fernández-Torres ◽  
Karina Martínez Flores ◽  
Víctor Hugo Cárdenas-Soria ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Cartilaginous Tissues

scholarly journals Encapsulated Allogeneic Synovial Membrane Mesenchymal Stem Cells Provide Better Outcomes of Chondral Lesions in Horses

2021 ◽  
Author(s):  
Vitor Hugo Santos ◽  
João Pedro Hübbe Pfeifer ◽  
Fernanda de Castro Stievani ◽  
Gustavo Santos Rosa ◽  
Emanuel Vitor Pereira Apolonio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Cartilage Degradation ◽  
Control Group ◽  
Lower Grade ◽  
Alginate Hydrogel ◽  
Chondral Lesions ◽  
Amyloid A ◽  
Fluid Analysis

Abstract BackgroundOsteoarthritis is the main cause of equine lameness and its treatment remains ineffective. Synovial membrane mesenchymal stem cells (SMMSC) provide satisfactory outcomes in joint injuries, mainly due to their immunomodulatory and reparative properties. This study aimed to evaluate the effect of MSMSC, either encapsulated in alginate hydrogel or free, in chondral lesions of horses.MethodsChondral lesions were surgically induced in medial trochlea of talus of fifteen horses. Animals were treated with PBS, 1x107 free SMMSC or 1x107 encapsulated SMMSC. Physical evaluations, lameness scores and synovial fluid analysis were determined (cytological analysis and dosage of IL-1, IL-10, IL-6, INF-Ɣ, TNF 𝛼, P Substance, Serum Amyloid A, TGF-β, IGF and PGE2) initially and followed for up to two weeks. Cartilage biopsies were performed 150 days after the induction for histological analysis and immunohistochemistry staining.ResultsAll groups presented inflammation initially. Although free SMMSC showed moderate tissue repair, encapsulated SMMSC modulated inflammation and had the lower grade of inflammation with superior tissue macro and microscopic aspects at the end, while the control group showed fibrosis and poor cartilage appearance. This study suggests better stem cell effectiveness in chondral defects when encapsulated MSCs are used.ConclusionsWhile the absence of treatment perpetuates cartilage degradation, encapsulated SMMSC responded better to the initial inflammation, interacting and modulating the environment through the release of anti-inflammatory cytokines. Better outcomes observed in encapsulated MSCs were related to the immuno and physical barrier provided by the alginate hydrogel, allowing a longer period of permanence and interaction between MSCs and the environment.

scholarly journals The Effect of Cyclic Hydrostatic Pressure on the Functional Development of Cartilaginous Tissues Engineered Using Bone Marrow Derived Mesenchymal Stem Cells

2011 ◽  
Author(s):  
Eric G. Meyer ◽  
Conor T. Buckley ◽  
Daniel J. Kelly
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilaginous Tissues ◽  
Functional Development ◽  
The Many ◽  
Interesting Alternative

Articular cartilage has a poor capacity for repair. Of the many procedures available to the orthopaedic surgeon, osteochondral grafting is the only technique which reliably produces hyaline cartilage within a defect.1 Bone marrow derived mesenchymal stem cells (MSCs) are an interesting alternative to harvesting cartilage grafts for chondrocytes as they also have the ability to produce cartilaginous tissues in vitro. This suggests that if tissue engineering strategies could be used to develop cartilaginous grafts with mechanical properties approaching that of normal articular cartilage, then hyaline tissue could be regenerated. Of concern with such approaches are reports that the mechanical properties of cartilaginous tissues engineered using MSCs are inferior to that engineered using chondrocytes derived from articular cartilage, although recent studies have demonstrated that adult equine MSCs produce a cartilaginous tissue mechanically superior to that derived using animal-matched adult chondrocytes.2

scholarly journals Multipotent mesenchymal stem cells from adult human synovial membrane

2001 ◽  
Vol 44 (8) ◽  
pp. 1928-1942 ◽  
Author(s):  
Cosimo De Bari ◽  
Francesco Dell'Accio ◽  
Przemyslaw Tylzanowski ◽  
Frank P. Luyten
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Adult Human ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Correction to: Culture of mesenchymal stem cells derived from equine synovial membrane in alginate hydrogel microcapsules

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Vitor Hugo Santos ◽  
João Pedro Hübbe Pfeifer ◽  
Jaqueline Brandão de Souza ◽  
Betsabéia Heloisa Gentilha Milani ◽  
Rogério Antonio de Oliveira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Alginate Hydrogel

scholarly journals Synovial membrane mesenchymal stem cells: past life, current situation, and application in bone and joint diseases

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Na Li ◽  
Jinfang Gao ◽  
Liangyu Mi ◽  
Gailian Zhang ◽  
Liyun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Cell Contact ◽  
Differentiation Potential ◽  
Existing Problems ◽  
Cell Layers ◽  
Past Life ◽  
Molecular Phenotypes ◽  
Fibroblast Like Synoviocytes

Abstract Mesenchymal stem cells (MSCs) can be isolated from not only bone marrow, but also various adult mesenchymal tissues such as periosteum, skeletal muscle, and adipose tissue. MSCs from different tissue sources have different molecular phenotypes and differentiation potential. Synovial membrane (SM) is an important and highly specific component of synovial joints. Previous studies have suggested that the synovium is a structure with a few cell layers thick and consists mainly of fibroblast-like synoviocytes (FLS), which forms a layer that lining the synovial membrane on the joint cavity and synovial fluid through cell-cell contact. In recent years, studies have found that there are also mesenchymal stem cells in the synovium, and as an important part of the mesenchymal stem cell family, it has strong capabilities of cartilage forming and tissue repairing. This article reviews the sources, surface markers, subtypes, influencing factors, and applications in inflammatory joints of synovial membrane mesenchymal stem cells (SM-MSCs) in recent years, aiming to clarify the research status and existing problems of SM-MSCs.

Allogeneic synovial membrane-derived mesenchymal stem cells do not significantly affect initial inflammatory parameters in a LPS-induced acute synovitis model

2020 ◽  
Vol 132 ◽  
pp. 485-491
Author(s):  
Gustavo Rosa ◽  
André Massahiro Teramoto Krieck ◽  
Enrico Padula ◽  
João Pedro Hübbe Pfeifer ◽  
Jaqueline Brandão de Souza ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Inflammatory Parameters
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