scholarly journals Correction to: Culture of mesenchymal stem cells derived from equine synovial membrane in alginate hydrogel microcapsules

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Vitor Hugo Santos ◽  
João Pedro Hübbe Pfeifer ◽  
Jaqueline Brandão de Souza ◽  
Betsabéia Heloisa Gentilha Milani ◽  
Rogério Antonio de Oliveira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Alginate Hydrogel

scholarly journals Encapsulated Allogeneic Synovial Membrane Mesenchymal Stem Cells Provide Better Outcomes of Chondral Lesions in Horses

2021 ◽  
Author(s):  
Vitor Hugo Santos ◽  
João Pedro Hübbe Pfeifer ◽  
Fernanda de Castro Stievani ◽  
Gustavo Santos Rosa ◽  
Emanuel Vitor Pereira Apolonio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Cartilage Degradation ◽  
Control Group ◽  
Lower Grade ◽  
Alginate Hydrogel ◽  
Chondral Lesions ◽  
Amyloid A ◽  
Fluid Analysis

Abstract BackgroundOsteoarthritis is the main cause of equine lameness and its treatment remains ineffective. Synovial membrane mesenchymal stem cells (SMMSC) provide satisfactory outcomes in joint injuries, mainly due to their immunomodulatory and reparative properties. This study aimed to evaluate the effect of MSMSC, either encapsulated in alginate hydrogel or free, in chondral lesions of horses.MethodsChondral lesions were surgically induced in medial trochlea of talus of fifteen horses. Animals were treated with PBS, 1x107 free SMMSC or 1x107 encapsulated SMMSC. Physical evaluations, lameness scores and synovial fluid analysis were determined (cytological analysis and dosage of IL-1, IL-10, IL-6, INF-Ɣ, TNF 𝛼, P Substance, Serum Amyloid A, TGF-β, IGF and PGE2) initially and followed for up to two weeks. Cartilage biopsies were performed 150 days after the induction for histological analysis and immunohistochemistry staining.ResultsAll groups presented inflammation initially. Although free SMMSC showed moderate tissue repair, encapsulated SMMSC modulated inflammation and had the lower grade of inflammation with superior tissue macro and microscopic aspects at the end, while the control group showed fibrosis and poor cartilage appearance. This study suggests better stem cell effectiveness in chondral defects when encapsulated MSCs are used.ConclusionsWhile the absence of treatment perpetuates cartilage degradation, encapsulated SMMSC responded better to the initial inflammation, interacting and modulating the environment through the release of anti-inflammatory cytokines. Better outcomes observed in encapsulated MSCs were related to the immuno and physical barrier provided by the alginate hydrogel, allowing a longer period of permanence and interaction between MSCs and the environment.

scholarly journals Encapsulated Allogeneic Synovial Membrane Mesenchymal Stem Cells Provide Better Outcomes of Chondral Lesions in Horses

2021 ◽  
Author(s):  
Vitor Hugo Santos ◽  
João Pedro Hübbe Pfeifer ◽  
Gustavo dos Santos Rosa ◽  
Fernanda de Castro Stievani ◽  
Emanuel Vitor Pereira Apolonio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Cartilage Degradation ◽  
Control Group ◽  
Lower Grade ◽  
Alginate Hydrogel ◽  
Chondral Lesions ◽  
Amyloid A ◽  
Fluid Analysis

Abstract Osteoarthritis is the main cause of equine lameness, and its treatment remains ineffective. Synovial membrane mesenchymal stem cells (SMMSCs) provide satisfactory outcomes in joint injuries, mainly due to their immunomodulatory and reparative properties. This study aimed to evaluate the effect of SMMSCs, either encapsulated in alginate hydrogel or free, in chondral lesions of horses.Methods: Chondral lesions were surgically induced in the medial trochlea of the talus of fifteen horses. Animals were treated with PBS free SMMSCs or encapsulated SMMSCs. Physical evaluations, assignment of lameness scores and synovial fluid analysis were performed (cytological analysis and dosage of IL-1, IL-10, IL-6, INF-Ɣ, TNF 𝛼, P substance, serum amyloid A, TGF-β, IGF and PGE2) for two weeks. Cartilage biopsies were performed 150 days after induction for histological analysis and immunohistochemistry staining.Results: All groups initially presented inflammation. Although free SMMSCs showed moderate tissue repair, encapsulated SMMSCs had a lower grade of inflammation with superior tissue macro- and microscopic aspects at the end, while the control group showed fibrosis and poor cartilage aspect. This study suggests better effectiveness of stem cells in chondral defects when encapsulated MSCs are used.Conclusion: While the absence of treatment perpetuates cartilage degradation, encapsulated SMMSCs respond better to initial inflammation, interacting and modulating the environment through the release of anti-inflammatory cytokines. Better outcomes observed in encapsulated MSCs were related to the immuno- and physical barriers provided by the alginate hydrogel, allowing a longer period of permanence and interaction between MSCs and the environment.

scholarly journals Culture of mesenchymal stem cells derived from equine synovial membrane in alginate hydrogel microcapsules

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Vitor Hugo Santos ◽  
João Pedro Hübbe Pfeifer ◽  
Jaqueline Brandão de Souza ◽  
Betsabéia Heloisa Gentilha Milani ◽  
Rogério Antonio de Oliveira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Membrane ◽  
Alginate Hydrogel

scholarly journals 14-3-3ε protein-loaded 3D hydrogels favor osteogenesis

2020 ◽  
Vol 31 (11) ◽  
Author(s):  
Ana A. Aldana ◽  
Marina Uhart ◽  
Gustavo A. Abraham ◽  
Diego M. Bustos ◽  
Aldo R. Boccaccini
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Printing ◽  
Osteogenic Differentiation ◽  
Alginate Hydrogel ◽  
Hydrogel Scaffolds ◽  
Future Developments ◽  
Positive Effect ◽  
Cell Adhesion And Proliferation

Abstract3D printing has emerged as vanguard technique of biofabrication to assemble cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissues. In this work, gelatin methacrylate (GelMA)/alginate hydrogel scaffolds were obtained by 3D printing and 14-3-3ε protein was encapsulated in the hydrogel to induce osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASC). GelMA/alginate-based grid-like structures were printed and remained stable upon photo-crosslinking. The viscosity of alginate allowed to control the pore size and strand width. A higher viscosity of hydrogel ink enhanced the printing accuracy. Protein-loaded GelMA/alginate-based hydrogel showed a clear induction of the osteogenic differentiation of hASC cells. The results are relevant for future developments of GelMA/alginate for bone tissue engineering given the positive effect of 14-3-3ε protein on both cell adhesion and proliferation.

Mesenchymal Stem Cells Delivered With Alginate Hydrogel Improves Conduction at the Border Zone of Healed MI

Heart Rhythm ◽  
2010 ◽  
Vol 7 (11) ◽  
pp. 1714
Author(s):  
Nikhil C. Panda ◽  
Sean Zuckerman ◽  
KeKe Fan ◽  
Devi Gopinath ◽  
David S. Rosenbaum ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Border Zone ◽  
Alginate Hydrogel

Abstract 558: Microchanneled Polysaccharide Hydrogel Laden With Endothelial Cells and Mesenchymal Stem Cells With VEGF Facilitate Formation of Vascular Structures and Are Effective for Repairing Tissue Ischemia

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Sangho Lee ◽  
Min Kyung Lee ◽  
Hyunjoon Kong ◽  
Young-sup Yoon
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Survival ◽  
Vascular Structure ◽  
Hindlimb Ischemia ◽  
Alginate Hydrogel ◽  
Vessel Formation

Various hydrogels are used to create vascular structure in vitro or to improve cell engraftment to overcome low cell survival in vivo, a main hurdle for bare cell therapy Recently we developed a modified alginate hydrogel within which microchannels are aligned to guide the direction and spatial organization of loaded cells. We investigated whether these cell constructs in which HUVECs and human mesenchymal stem cells (hMSCs) are co-loaded in this novel microchanneled hydrogel facilitate formation of vessels in vitro and in vivo, and enhance recovery of hindlimb ischemia. We crafted a modified alginate hydrogel which has microchannels, incorporates a cell adhesion peptide RGD, and was encapsulated with VEGF. We then compared vascular structure formation between the HUVEC only (2 x 105 cells) group and the HUVEC plus hMSC group. In the HUVEC+hMSC group, we mixed HUVECs and hMSCs at the ratio of 3:1. For cell tracking, we labeled HUVECs with DiO, a green fluorescence dye. After loading cells into the microchannels of the hydrogel, these constructs were cultured for seven days and were examined by confocal microscopy. In the HUVEC only group, HUVECs stands as round shaped cells without forming tubular structures within the hydrogel. However, in the HUVEC+hMSC group, HUVECs were stretched out and connected with each other, and formed vessel-like structure following pre-designed microchannels. These results suggested that hMSCs play a critical role for vessel formation by HUVECs. We next determined their in vivo effects using a mouse hindlimb ischemia model. We found that engineered HUVEC+hMSC group showed significantly higher perfusion over 4 weeks compared to the engineered HUVEC only group or bare cell (HUVEC) group. Confocal microscopic analysis of harvested tissues showed more robust vessel formation within and outside of the cell constructs and longer term cell survival in HUVEC+hMSC group compared to the other groups. In conclusion, this novel microchanneled alginate hydrogel facilitates aligned vessel formation of endothelial cells when combined with MSCs. This vessel-embedded hydrogel constructs consisting of HUVECs and MSCs contribute to perfusable vessel formation, prolong cell survival in vivo, and are effective for recovering limb ischemia.

scholarly journals Dental mesenchymal stem cells encapsulated in an alginate hydrogel co-delivery microencapsulation system for cartilage regeneration

2013 ◽  
Vol 9 (12) ◽  
pp. 9343-9350 ◽  
Author(s):  
Alireza Moshaverinia ◽  
Xingtian Xu ◽  
Chider Chen ◽  
Kentaro Akiyama ◽  
Malcolm L. Snead ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Regeneration ◽  
Alginate Hydrogel

scholarly journals Application of bioderadable materials based on alginate and pectin to prevent the formation of peritoneal adhesions

2019 ◽  
Vol 16 (1) ◽  
pp. 46-55
Author(s):  
A. V. Zhura ◽  
V. I. Kulikouskaya ◽  
K. S. Hileuskaya ◽  
A. N. Kraskouski ◽  
S. I. Tretyak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Freeze Drying ◽  
Physiological Solution ◽  
Solution Casting ◽  
Casting Method ◽  
Alginate Hydrogel ◽  
Peritoneal Adhesions ◽  
Mesenchymal Stem Cell Transplantation ◽  
Solution Casting Method

Treatment of peritoneal adhesions are still of great importance today. One of the prophylactic measures is biodegradable gels and membranes. The objective of the investigation was to develop and to experimentally assess new materials based on pectin and alginate. Alginate hydrogel was prepared with 4.0, 7.0 and 10.0 weight per cent. The pectin sols were synthesized by the “green chemistry” method. To make flms and porous membranes the solution casting method and the freeze-drying technique were used accordingly. The materials were studied in vitro and in vivo. Their physical properties, biocompatibility, biodegradability, adhesion, the prevention effect, the possibility of using as a matrix for mesenchymal stem cell transplantation were assessed. Alginate hydrogel of 7.0 weight per cent didn’t cause postoperative complications and led to low adhesions incidence – in 10 % of cases (in the comparison group – 85.7 %). Pectin flms obtained by the solution casting method became deformed already in the physiological solution. Biodegradation of the flms was absent in the experiment, abscesses and infltrates in the abdominal cavity were noted. Mesenchymal stem cells didn’t attach to such flms. Porous pectin matrices synthetized by the freeze-drying technique became partially decomposed already in the physiological solution. In the experiment, these membranes were biodegraded in half animals with the formation of mild adhesions only in 25 %. Mesenchymal stem cells showed a good attachment to their surface. The developed materials based on alginate gel and porous pectin membranes showed a high biodegradation, good biocompatibility, adhesion the prevention effect and the possibility of using as a matrix for stem cells transplantation.

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