Role of Cytoskeleton Proteins in the Morphological Changes During Apoptotic Cell Death of Cerebellar Granule Neurons

2010 ◽  
Vol 36 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Alette Ortega ◽  
Julio Morán
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Apoptotic Cell Death ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule

Role of Ionic Fluxes in the Apoptotic Cell Death of Cultured Cerebellar Granule Neurons

2004 ◽  
Vol 29 (1) ◽  
pp. 227-238 ◽  
Author(s):  
A. Franco-Cea ◽  
A. Valencia ◽  
S. Sánchez-Armass ◽  
G. Domínguez ◽  
J. Morán
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Ionic Fluxes

Lysophosphatidylcholine protects cerebellar granule neurons from apoptotic cell death

2009 ◽  
Vol 87 (1) ◽  
pp. 190-199 ◽  
Author(s):  
Yutaka Ikeno ◽  
So-hyun Cheon ◽  
Naoko Konno ◽  
Ayako Nakamura ◽  
Katsuhiko Kitamoto ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule

Apoptotic cell death of cerebellar granule neurons in genetically ataxia (ax) mice

2000 ◽  
Vol 288 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Makoto Ohgoh ◽  
Kazuto Yamazaki ◽  
Hiroo Ogura ◽  
Yukio Nishizawa ◽  
Isao Tanaka
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule

Functional Validation of DownRegulated MicroRNAs in HeLa Cells Treated with Polyalthia longifolia Leaf Extract Using Different Microscopic Approaches: A Morphological Alteration-Based Validation

2019 ◽  
Vol 25 (05) ◽  
pp. 1263-1272 ◽  
Author(s):  
Shanmugapriya ◽  
Soundararajan Vijayarathna ◽  
Sreenivasan Sasidharan
Keyword(s):  
Cell Death ◽  
Electron Microscope ◽  
Hela Cells ◽  
Leaf Extract ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Apoptotic Cell Death ◽  
Morphological Alteration ◽  
Polyalthia Longifolia

AbstractSeveral microscopy methods have been developed to assess the morphological changes in cells in the investigations of the mode of cell death in response to a stimulus. Our recent finding on the treatment of the IC50 concentration (26.67 μg/mL) of Polyalthia longifolia leaf extract indicated the induction of apoptotic cell death via the regulation of miRNA in HeLa cells. Hence, the current study was conducted to validate the function of these downregulated microRNAs in P. longifolia-treated HeLa cells using microscopic approaches. These include scanning electron microscope (SEM), transmission electron microscope (TEM), and acridine orange/propidium iodide (AO/PI)-based fluorescent microscopy techniques by observing the morphological alterations to cells after transfection with mimic miRNA. Interestingly, the morphological changes observed in this study demonstrated the apoptotic hallmarks, for instance, cell blebbing, cell shrinkage, cytoplasmic and nuclear condensation, vacuolization, cytoplasmic extrusion, and the formation of apoptotic bodies, which proved the role of dysregulated miRNAs in apoptotic HeLa cell death after treatment with the P. longifolia leaf extract. Conclusively, the current study proved the crucial role of downregulated miR-484 and miR-221-5p in the induction of apoptotic cell death in P. longifolia-treated HeLa cells using three approaches—SEM, TEM, and AO/PI-based fluorescent microscope.

Sign in / Sign up

Export Citation Format

Share Document