scholarly journals A Comparison of Drug Transport in Pulmonary Absorption Models: Isolated Perfused rat Lungs, Respiratory Epithelial Cell Lines and Primary Cell Culture

2017 ◽  
Vol 34 (12) ◽  
pp. 2532-2540 ◽  
Author(s):  
Cynthia Bosquillon ◽  
Michaela Madlova ◽  
Nilesh Patel ◽  
Nicola Clear ◽  
Ben Forbes
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Drug Transport ◽  
Primary Cell Culture ◽  
Pulmonary Absorption ◽  
Respiratory Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Absorption Models ◽  
Respiratory Epithelial

Protein glutathionylation in respiratory epithelial cell lines

Pneumologie ◽  
2004 ◽  
Vol 58 (S 1) ◽  
Author(s):  
N Dauletbaev ◽  
S Kubow ◽  
LC Lands
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Respiratory Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Respiratory Epithelial

Incorporation of α-linolenic acid and linoleic acid into human respiratory epithelial cell lines

Lipids ◽  
2001 ◽  
Vol 36 (7) ◽  
pp. 713-717 ◽  
Author(s):  
Dani-Louise Bryan ◽  
Prue Hart ◽  
Kevin Forsyth ◽  
Robert Gibson
Keyword(s):  
Linoleic Acid ◽  
Epithelial Cell ◽  
Linolenic Acid ◽  
Cell Lines ◽  
Respiratory Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Respiratory Epithelial

scholarly journals Differential Responses by Human Respiratory Epithelial Cell Lines to Respiratory Syncytial Virus Reflect Distinct Patterns of Infection Control

2018 ◽  
Vol 92 (15) ◽  
Author(s):  
Philippa Hillyer ◽  
Rachel Shepard ◽  
Megan Uehling ◽  
Mina Krenz ◽  
Faruk Sheikh ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Lines ◽  
A549 Cells ◽  
Type I ◽  
Epithelial Cell Lines ◽  
Rsv Infection ◽  
Syncytial Virus ◽  
Respiratory Epithelial

ABSTRACT Respiratory syncytial virus (RSV) infects small foci of respiratory epithelial cells via infected droplets. Infection induces expression of type I and III interferons (IFNs) and proinflammatory cytokines, the balance of which may restrict viral replication and affect disease severity. We explored this balance by infecting two respiratory epithelial cell lines with low doses of recombinant RSV expressing green fluorescent protein (rgRSV). A549 cells were highly permissive, whereas BEAS-2B cells restricted infection to individual cells or small foci. After infection, A549 cells expressed higher levels of IFN-β-, IFN-λ-, and NF-κB-inducible proinflammatory cytokines. In contrast, BEAS-2B cells expressed higher levels of antiviral interferon-stimulated genes, pattern recognition receptors, and other signaling intermediaries constitutively and after infection. Transcriptome analysis revealed that constitutive expression of antiviral and proinflammatory genes predicted responses by each cell line. These two cell lines provide a model for elucidating critical mediators of local control of viral infection in respiratory epithelial cells. IMPORTANCE Airway epithelium is both the primary target of and the first defense against respiratory syncytial virus (RSV). Whether RSV replicates and spreads to adjacent epithelial cells depends on the quality of their innate immune responses. A549 and BEAS-2B are alveolar and bronchial epithelial cell lines, respectively, that are often used to study RSV infection. We show that A549 cells are permissive to RSV infection and express genes characteristic of a proinflammatory response. In contrast, BEAS-2B cells restrict infection and express genes characteristic of an antiviral response associated with expression of type I and III interferons. Transcriptome analysis of constitutive gene expression revealed patterns that may predict the response of each cell line to infection. This study suggests that restrictive and permissive cell lines may provide a model for identifying critical mediators of local control of infection and stresses the importance of the constitutive antiviral state for the response to viral challenge.

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