Both the constitutive Cauliflower Mosaic Virus 35S and tissue-specific AGAMOUS enhancers activate transcription autonomously in Arabidopsis thaliana

2010 ◽  
Vol 74 (3) ◽  
pp. 293-305 ◽  
Author(s):  
Stacy D. Singer ◽  
Kerik D. Cox ◽  
Zongrang Liu
Keyword(s):  
Arabidopsis Thaliana ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Tissue Specific ◽  
Cauliflower Mosaic Virus 35S

scholarly journals Continual Green-Fluorescent Protein Monitoring of Cauliflower Mosaic Virus 35S Promoter Activity in Nematode-Induced Feeding Cells in Arabidopsis thaliana

1997 ◽  
Vol 10 (3) ◽  
pp. 394-400 ◽  
Author(s):  
Peter E. Urwin ◽  
Simon G. Møller ◽  
Catherine J. Lilley ◽  
Michael J. McPherson ◽  
Howard J. Atkinson
Keyword(s):  
Arabidopsis Thaliana ◽  
Green Fluorescent Protein ◽  
Mosaic Virus ◽  
Promoter Activity ◽  
Fluorescent Protein ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Feeding Sites ◽  
Green Fluorescent ◽  
Cauliflower Mosaic Virus 35S

The responsiveness of the cauliflower mosaic virus 35S promoter in feeding sites developed by both sexes of Heterodera schachtii and female Meloidogyne incognita has been studied. The objective was to establish the value of green-fluorescent protein (GFP) as a nondestructive reporter gene system for characterizing promoter activity at nematode feeding sites in vivo. Growth units were devised that allowed individual feeding sites in roots of Arabidopsis thaliana to be observed by both bright-field and epifluorescent illumination. Changes in GFP expression were visually observed under experimental conditions that resulted in chloroplast formation in syncytia but not other root cells. Changes in GFP levels altered the extent of quenching, by this protein, of red light emitted by chlorophyll within the chloroplasts under violet excitation. Image analysis provided a semiquantitative basis for simultaneous measurement of changes in GFP fluorescence and the unquenched emission by chlorophyll. GFP levels were constant in cells surrounding the syncytium induced by H. schachtii, but they fell progressively from 10 to 35 days postinfection within this structure. Significant reduction in GFP levels was not limited to the early part of the time course but also occurred between 27 and 35 days postinfection. GFP was detected by immunoblotting in females of M. incognita but not in H. schachtii parasitizing similar GFP-expressing roots.

Differential inhibition of downstream gene expression by the cauliflower mosaic virus 35S RNA leader

Virus Genes ◽  
1989 ◽  
Vol 3 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Johannes F�tterer ◽  
Karl Gordon ◽  
Pierre Pfeiffer ◽  
H�l�lene Sanfa�on ◽  
Barbara Pisan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Differential Inhibition ◽  
Downstream Gene Expression ◽  
Cauliflower Mosaic Virus 35S

scholarly journals The 5′ Third of Cauliflower mosaic virus Gene VI Conditions Resistance Breakage in Arabidopsis Ecotype Tsu-0

2002 ◽  
Vol 92 (2) ◽  
pp. 190-196 ◽  
Author(s):  
K. Agama ◽  
S. Beach ◽  
J. Schoelz ◽  
S. M. Leisner
Keyword(s):  
Arabidopsis Thaliana ◽  
Mosaic Virus ◽  
Viral Genome ◽  
Plant Defenses ◽  
Cauliflower Mosaic Virus ◽  
Virus Gene ◽  
Arabidopsis Ecotype ◽  
Passive Mechanism ◽  
Different Types ◽  
Solanaceous Plants

Arabidopsis thaliana ecotypes vary in their responses to viruses. In this study, we analyzed the variation in response of A. thaliana ecotype Tsu-0 to Cauliflower mosaic virus (CaMV). This ecotype was previously reported to be resistant to two CaMV isolates (CM1841 and CM4-184), but susceptible to W260. In this study, we show that Tsu-0 is resistant to four additional CaMV isolates. CaMV propagated within the rosette leaves of Tsu-0 plants, but did not appear to spread systemically into the inflorescence. However, virus viability in rosette leaves of Tsu-0 plants apparently was not compromised because infectious CaMV could be recovered from these organs. W260 overcomes Tsu-0 resistance by a passive mechanism (i.e., this virus avoids activating plant defenses). The portion of the viral genome responsible for W260 resistance breakage was mapped to the 5′ third of gene VI, which we have termed RBR-1. This region is also responsible for controlling the ability of CaMV to infect different types of solanaceous plants. Hence, the pathways by which plants of different families interact with CaMV may be conserved through evolution.

scholarly journals The Cauliflower Mosaic Virus 35S Promoter Extends into the Transcribed Region

2004 ◽  
Vol 78 (22) ◽  
pp. 12120-12128 ◽  
Author(s):  
Sandra Pauli ◽  
Helen M. Rothnie ◽  
Gang Chen ◽  
Xiaoyuan He ◽  
Thomas Hohn
Keyword(s):  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Sequence Motifs ◽  
Transcription Start ◽  
Transcriptional Enhancers ◽  
Enhance Gene Expression ◽  
Enhancer Function ◽  
Nucleotide Region ◽  
Cauliflower Mosaic Virus 35S

ABSTRACT A 60-nucleotide region (S1) downstream of the transcription start site of the cauliflower mosaic virus 35S RNA can enhance gene expression. By using transient expression assays with plant protoplasts, this activity was shown to be at least partially due to the effect of transcriptional enhancers within this region. We identify sequence motifs with enhancer function, which are normally masked by the powerful upstream enhancers of the 35S promoter. A repeated CT-rich motif is involved both in enhancer function and in interaction with plant nuclear proteins. The S1 region can also enhance expression from heterologous promoters.

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