Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids

In this study we present evidence that one of two reactions of the xanthophyll cycle, violaxanthin de-epoxidation, may occur in unilamellar egg phosphatidylcholine vesicles supplemented with monogalactosyldiacylglycerol (MGDG). Activity of violaxanthin de-epoxidase (VDE) in this system was found to be strongly dependent on the content of MGDG in the membrane; however, only to a level of 30 mol%. Above this concentration the rate of violaxanthin de-epoxidation decreased. The effect of individual thylakoid lipids on VDE-independent violaxanthin transformation was also investigated and unspecific effects of phosphatidylglycerol and sulphoquinovosyldiacyglycerol, probably related to the acidic character of these lipids, were found. The presented results suggest that violaxanthin de-epoxidation most probably takes place inside MGDG-rich domains of the thylakoid membrane. The described activity of the violaxanthin de-epoxidation reaction in liposomes opens new possibilities in the investigation of the xanthophyll cycle and may contribute to a better understanding of this process.

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Specific role of phosphatidylglycerol and functional overlaps with other thylakoid lipids in Arabidopsis chloroplast biogenesis

Plant Cell Reports ◽

10.1007/s00299-014-1719-z ◽

2014 ◽

Vol 34 (4) ◽

pp. 631-642 ◽

Cited By ~ 29

Author(s):

Koichi Kobayashi ◽

Sho Fujii ◽

Mayuko Sato ◽

Kiminori Toyooka ◽

Hajime Wada

Keyword(s):

Specific Role ◽

Chloroplast Biogenesis ◽

Thylakoid Lipids

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Thermal stability of trimeric light-harvesting chlorophyll a/b complex (LHCIIb) in liposomes of thylakoid lipids

Biochimica et Biophysica Acta (BBA) - Bioenergetics ◽

10.1016/j.bbabio.2006.08.010 ◽

2006 ◽

Vol 1757 (12) ◽

pp. 1642-1648 ◽

Cited By ~ 41

Author(s):

Chunhong Yang ◽

Stephanie Boggasch ◽

Winfried Haase ◽

Harald Paulsen

Keyword(s):

Thermal Stability ◽

Chlorophyll A ◽

Light Harvesting ◽

Thermal Stability Of ◽

Thylakoid Lipids

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Molecular Order and Dynamics in Membranes Containing α-Tocopherol, (trans 16:3)-Phosphatidyl-Glycerol and other Thylakoid Lipids

Current Research in Photosynthesis ◽

10.1007/978-94-009-0511-5_391 ◽

1990 ◽

pp. 1715-1718

Author(s):

G. Van Ginkel ◽

L. J. Korstanje ◽

R. Moormans ◽

G. Pruijsen ◽

M. Van Zandvoort ◽

...

Keyword(s):

Phosphatidyl Glycerol ◽

Molecular Order ◽

Thylakoid Lipids

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Actaptive plasticity of the thylakoid lipids in diuron-resistant Euglena gracilis

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism ◽

10.1016/0005-2760(86)90345-0 ◽

1986 ◽

Vol 878 (1) ◽

pp. 71-78 ◽

Cited By ~ 6

Author(s):

Didier Troton ◽

Regis Calvayrac ◽

Danielle Laval-Martin

Keyword(s):

Euglena Gracilis ◽

Thylakoid Lipids

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Chloroplast lipid synthesis and lipid trafficking through ER–plastid membrane contact sites

Biochemical Society Transactions ◽

10.1042/bst20110752 ◽

2012 ◽

Vol 40 (2) ◽

pp. 457-463 ◽

Cited By ~ 82

Author(s):

Zhen Wang ◽

Christoph Benning

Keyword(s):

Fatty Acids ◽

Current Knowledge ◽

Lipid Transfer ◽

Photosynthetic Membrane ◽

Lipid Trafficking ◽

Membrane Contact Sites ◽

Contact Sites ◽

Membrane Contact ◽

Envelope Membranes ◽

Thylakoid Lipids

Plant chloroplasts contain an intricate photosynthetic membrane system, the thylakoids, and are surrounded by two envelope membranes at which thylakoid lipids are assembled. The glycoglycerolipids mono- and digalactosyldiacylglycerol, and sulfoquinovosyldiacylglycerol as well as phosphatidylglycerol, are present in thylakoid membranes, giving them a unique composition. Fatty acids are synthesized in the chloroplast and are either directly assembled into thylakoid lipids at the envelope membranes or exported to the ER (endoplasmic reticulum) for extraplastidic lipid assembly. A fraction of lipid precursors is reimported into the chloroplast for the synthesis of thylakoid lipids. Thus polar lipid assembly in plants requires tight co-ordination between the chloroplast and the ER and necessitates inter-organelle lipid trafficking. In the present paper, we discuss the current knowledge of the export of fatty acids from the chloroplast and the import of chloroplast lipid precursors assembled at the ER. Direct membrane contact sites between the ER and the chloroplast outer envelopes are discussed as possible conduits for lipid transfer.

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