Somatic embryogenesis in loblolly pine (Pinus taeda) and Douglas fir (Pseudotsuga menziesii): improving culture initiation and growth with MES pH buffer, biotin, and folic acid

2005 ◽  
Vol 80 (1) ◽  
pp. 91-103 ◽  
Author(s):  
Gerald S. Pullman ◽  
S. Johnson ◽  
S. Van Tassel ◽  
Y. Zhang
Keyword(s):  
Somatic Embryogenesis ◽  
Folic Acid ◽  
Pinus Taeda ◽  
Pseudotsuga Menziesii ◽  
Loblolly Pine ◽  
Douglas Fir ◽  
Culture Initiation ◽  
Ph Buffer

Anchored Reference Loci in Loblolly Pine (Pinus taeda L.) for Integrating Pine Genomics

Genetics ◽  
2001 ◽  
Vol 159 (2) ◽  
pp. 799-809 ◽  
Author(s):  
Garth R Brown ◽  
Edward E Kadel ◽  
Daniel L Bassoni ◽  
Kristine L Kiehne ◽  
Berhanu Temesgen ◽  
...  
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Comparative Mapping ◽  
Expressed Sequence Tag ◽  
Single Species ◽  
Douglas Fir ◽  
Genetic Maps ◽  
Multiple Sources ◽  
Primer Sets ◽  
Pinus Taeda L

Abstract Anchored reference loci provide a framework for comparative mapping. They are landmarks to denote conserved chromosomal segments, allowing the synthesis of genetic maps from multiple sources. We evaluated 90 expressed sequence tag polymorphisms (ESTPs) from loblolly pine (Pinus taeda L.) for this function. Primer sets were assayed for amplification and polymorphism in six pedigrees, representing two subgenera of Pinus and a distant member of the Pinaceae, Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco). On average, 89% of primer sets amplified in four species of subgenus Pinus, 49% in one species of subgenus Strobus, and 22% in Douglas-fir. Polymorphisms were detected for 37–61% of the ESTPs within each pedigree. Comparative mapping in loblolly and slash pine (P. elliottii Englm.) revealed that ESTPs mapped to the same location. Disrupted synteny or significant disruptions in colinearity were not detected. Thirty-five ESTPs met criteria established for anchor loci. The majority of those that did not meet these criteria were excluded when map location was known in only a single species. Anchor loci provide a unifying tool for the community, facilitating the creation of a “generic” pine map and serving as a foundation for studies on genome organization and evolution.

scholarly journals Infection of Select Pinaceae and Cupressaceae Seedlings to Phytophthora pinifolia

2019 ◽  
Vol 20 (2) ◽  
pp. 106-111
Author(s):  
Timothy L. Widmer ◽  
Stephen C. Dodge
Keyword(s):  
United States ◽  
At Risk ◽  
Pseudotsuga Menziesii ◽  
Loblolly Pine ◽  
Pinus Radiata ◽  
Pinus Elliottii ◽  
The United States ◽  
Douglas Fir ◽  
Important Species ◽  
Monterey Pine

Phytophthora pinifolia caused a devastating disease on Monterey pine (Pinus radiata) in Chile. This pathogen has not been reported in the United States, but there is concern should it arrive. There is little information regarding other hosts besides Monterey pine that may be susceptible to this pathogen. In the present study, other potential hosts within the Pinaceae and Cupressaceae were inoculated with zoospores of P. pinifolia and observed for symptoms and infection after 4 weeks. Similar to Monterey pine, knobcone (Pinus attenuata), bishop (P. muricata), and ponderosa (P. ponderosa) pines were highly infectious, whereas several important species such as loblolly pine (P. taeda) and Douglas fir (Pseudotsuga menziesii) showed no infection. P. pinifolia was also not recovered from slash (Pinus elliottii) and eastern white (P. strobus) pines. This study is important because it demonstrates other economically important tree hosts are at risk by P. pinifolia, should it enter the United States.

Somatic Embryogenesis in Douglas-fir (Pseudotsuga Menziesii)

1995 ◽  
pp. 303-313 ◽  
Author(s):  
Pramod K. Gupta ◽  
Roger Timmis ◽  
Kathleen A. Timmis ◽  
William C. Carlson ◽  
Elaine D. E. Welty
Keyword(s):  
Somatic Embryogenesis ◽  
Pseudotsuga Menziesii ◽  
Douglas Fir

Loblolly pine (Pinus taeda L.) somatic embryogenesis: maturation improvements by metal analyses of zygotic and somatic embryos

Plant Science ◽  
2003 ◽  
Vol 164 (6) ◽  
pp. 955-969 ◽  
Author(s):  
Gerald S Pullman ◽  
Paul Montello ◽  
John Cairney ◽  
Nanfei Xu ◽  
Xiaorong Feng
Keyword(s):  
Somatic Embryogenesis ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Pinus Taeda L
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