An experimental study on changes in sound absorption capability of spruce (Picea sitchensis), Douglas fir (Pseudotsuga menziesii), and larch (Larix kaempferi) after microwave treatment

This study investigated changes in the sound absorption coefficients of three anatomical sections of cubed spruce (Picea sitchensis), Douglas fir (Pseudotsuga menziesii), and larch (Larix kaempferi) after microwave treatment. Microwave treatment at 1000 W and 2.4 GHz for 20 min increased the sound absorption coefficients (at 2000–5000 Hz) of spruce by 6.9% in the transverse section, 20.0% in the radial section, and 31.7% in the tangential section. The sound absorption coefficients of Douglas fir increased by 28.9% in the transverse section, 19.1% in the radial section, and 50.0% in the tangential section. Larch coefficients increased by 16.7% in the transverse section, 37.2% in the radial section, and 38.8% in the tangential section. The sound absorption coefficients of the softwoods differed according to species and anatomical plane after microwave treatment. It was concluded that changes in the measured sound absorption coefficient indicate alteration in the pore structure of wood, which can affect in turn wood permeability and impregnation. These data will be helpful for predicting the permeability and impregnation of wood after microwave treatment.

Patrones de distribución espacial del arbolado en un bosque de Pseudotsuga menziesii en Chihuahua, México

El objetivo de este estudio fue caracterizar los patrones de distribución espacial de un bosque maduro en el estado de Chihuahua, México, donde coexisten coníferas de importancia para la conservación como Pseudotsuga, Picea y Abies. Se utilizó un análisis de patrón de puntos para evaluar mezcla de especies, diferenciación y dominancia dimensional. Los datos se obtuvieron de ocho sitios de muestreo de 1000 m2. Se analizaron de manera general 203 grupos estructurales, Pseudotsuga generó 84, Quercus 22 y Pinus 42, reflejando una distribución aleatoria de acuerdo con el índice de uniformidad de ángulos (Wi), mientras que el grado de mezcla (Mi) manifestó que la especie referencia cuenta con más de dos vecinos de diferente especie. No se registraron diámetros y alturas dominantes de manera general ya que (UDi) y (UHi) mostraron valores similares en cada uno de los grados de dominancia, la diferenciación dimensional (TDi), (THi) fue moderada, similar a estudios en bosques perturbados y bajo manejo. El conocimiento de la dinámica de bosques maduros es vital para una descripción precisa de las relaciones entre especies de manera más auténtica y puede ser base para la conservación o futuras restauraciones en áreas perturbadas.

Mycobiota of Fine Roots of Pseudotsuga menziesii Introduced to the Native Forest Environment

The mycobiota of the fine roots of Pseudotsuga menziesii were studied as a measure of the adaptation of this alien species to new soil and climatic conditions. We hypothesized that after approximately 130 years of growth in a given habitat, the fungal community colonizing the fine roots of introduced trees would resemble the biota of Pinus sylvestris and Fagus sylvatica in surrounding stands of similar age and site conditions. The genetic material isolated from the fine roots was subjected to metagenomic analysis. We recorded 33, 97 and 95 OTUs exclusively from root samples of Douglas fir, beech and pine, respectively; 124 were common to all sample types. The biota from the roots of P. menziesii featured a less diverse taxonomic composition and were characterized by the highest proportion of symbiotrophs (71.8%) versus saprothrophs (5.6%) and pathogens (0.24%). Some fungal taxa (19) in the roots of P. menziesii were common with the biota in the roots of other adjacent trees, while some (7) were unique to Douglas fir. Our results indicate a locally differentiated strategy of naturalness of fungi inhabiting soil and roots of P. menziesii, although 130 years have passed since the introduction of the species.

Predicting Present and Future Suitable Climate Spaces (Potential Distributions) for an Armillaria Root Disease Pathogen (Armillaria solidipes) and Its Host, Douglas-fir (Pseudotsuga menziesii), Under Changing Climates

Climate change and associated disturbances are expected to exacerbate forest root diseases because of altered distributions of existing and emerging forest pathogens and predisposition of trees due to climatic maladaptation and other disturbances. Predictions of suitable climate space (potential geographic distribution) for forest pathogens and host trees under contemporary and future climate scenarios will guide the selection of appropriate management practices by forest managers to minimize adverse impacts of forest disease within forest ecosystems. A native pathogen (Armillaria solidipes) that causes Armillaria root disease of conifers in North America is used to demonstrate bioclimatic models (maps) that predict suitable climate space for both pathogen and a primary host (Pseudotsuga menziesii, Douglas-fir) under contemporary and future climate scenarios. Armillaria root disease caused by A. solidipes is a primary cause of lost productivity and reduced carbon sequestration in coniferous forests of North America, and its impact is expected to increase under climate change due to tree maladaptation. Contemporary prediction models of suitable climate space were produced using Maximum Entropy algorithms that integrate climatic data with 382 georeferenced occurrence locations for DNA sequence-confirmed A. solidipes. A similar approach was used for visually identified P. menziesii from 11,826 georeferenced locations to predict its climatic requirements. From the contemporary models, data were extrapolated through future climate scenarios to forecast changes in geographic areas where native A. solidipes and P. menziesii will be climatically adapted. Armillaria root disease is expected to increase in geographic areas where predictions suggest A. solidipes is well adapted and P. menziesii is maladapted within its current range. By predicting areas at risk for Armillaria root disease, forest managers can deploy suitable strategies to reduce damage from the disease.

Pinaceae Species: Spruce, Pine and Fir as a New Culinary Herb and Spice

The Pinaceae family has traditionally been used as medicine, resorted to as a famine food and for ornamental purposes as Christmas trees. In the last few years numerous restaurants have been using different species of Pinaceae family as a garnish or an aromatic spice, using them in different culinary applications like oils and infusions to flavor dressings and broths. Abies grandis (Grand fir), Pseudotsuga menziesii (Douglas fir), Pinus sylvestris (Scots pine) and Picea abies (Norway spruce) were researched on taxonomy, habitats and non-edible uses, culinary traditions, health and nutritional properties, aroma profile. The main compounds in Pinaceae family are monoterpenes, oxygenated monoterpenes, sesquiterpenes, oxygenate sesquiterpenes, diterpenes and hydrocarbons, especially α-β-pinene, limonene, α-terpinene, and even bornyl acetate, responsible for aroma compounds such as citrusy-, woody-, herbal-, or piney aromas. Modern gastronomy uses, sensory analysis and culinary applications were applied for demonstrating the possibilities on modern culinary application in this novel yet traditional spice.

Morphological variability of Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) cones in the context of seed extraction

In the paper generating curves given by fourth-degree polynomials were used to model the shape of Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) cones from the Polish Forest Districts of Kołaczyce (one batch) and Opole (two batches), and to calculate the surface area and volume of individual cones. However, it was not possible to construct generalized equations for the surface area and volume of Douglas fir cones due to the high variability of empirical coefficients. The surface area and volume of the cones were also calculated from their length and diameter based on formulas for a cylinder and a barrel corrected by constants k1 and k2. The mean surface area of closed Douglas fir cones determined for the first, second, and third batch using the generating function was 4,348.4 mm2, 3,857.0 mm2, and 2,844.7 mm2, and the volume was 27,212.4 mm3, 21,012.9 mm3, and 12,844.4 mm3, respectively. The corresponding values calculated from the geometric formulas for solids were 4,332.0 mm2, 3,838.0 mm2, and 2,862.9 mm2 for the surface area and 27,366.0 mm3, 20,648.9 mm3, and 13,375.3 mm3 for the volume. The evaporation area of open cones was found to be five times greater than that of closed cones, with the difference being statistically significant. The outer and inner surfaces of scales taken from the middle segment of Douglas fir cones were photographed using a Quanta 200 scanning microscope (FEIC). The characteristic elements of scale morphology were evaluated by means of MultiScan Base software package. The outer and inner surfaces of Douglas fir scales were found to differ in some important ways, similarly as it has been reported in the literature for the Scots pine, silver fir, European larch, and black alder. The outer surface of scales is formed by thick-walled cells with marked protrusions, while the inner surface reveals cells with thin, frayed walls in the region adjacent to the seeds and wings. Knowledge of the geometry of Douglas fir cones and the morphology of their scales may be helpful in optimizing seed extraction parameters for those cones. Key words: seed extraction, model, shape curve, surface area, volume, scanning electron microscope

Dynamics of Rare Earths and associated major and trace elements during Douglas-fir (Pseudotsuga menziesii) and European beech (Fagus sylvatica L.) litter degradation

Given the diverse physico-chemical properties of elements, we hypothesize that their incoherent distribution across the leaf tissues, combined with the distinct resistance to degradation that each tissue exhibits, leads to distinct turnover rates between elements. Moreover, litter layers of different ages produce diverse chemical signatures in solution during the wet degradation. To verify our hypothesis, Na, K, Mg, Mn, Ca, Pb, Al and Fe were analysed together with the Rare Earth Elements (REE) in the solid fractions and in the respective leachates of fresh leaves and different humus layers of two forested soils developed under Pseudotsuga menziesii and Fagus sylvatica L. trees. The results from the leaching experiment were also compared to the in situ REE composition of the soil solutions to clarify the impact that the litter degradation processes may have on soil solution chemical compositions. Our results clearly show that REE, Al, Fe and Pb were preferentially retained in the solid litter material, in comparison to the other cations, and that their concentrations increased over time during the litter degradation. Accordingly, different litter fractions produced different yields of elements and REE patterns in the leachates, indicating that the tree species and the age of the litter play a role in the chemical release during the degradation. In particular, the evolution of the REE patterns according to the age of the litter layers allowed us to deliver new findings on REE fractionation and mobilization during litter degradation. In particular, the LaN/YbN ratio highlights differences in litter degradation intensity between both tree species, which was not shown with major cations. We finally showed the primary control effect that litter degradation can have on the REE composition of the soil solution, which presents a positive Ce anomaly associated with the dissolution and/or transportation of Ce-enriched MnO2 particles accumulated onto the surface of the old litter due to white fungi activity. Similar MREE and HREE enrichments were also found in the leachates and the soil solution, probably due to their higher affinity to the organic acids, which represent the primary products from the organic matter degradation.

Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species

This study aimed to establish the essential oil (EO) composition from young shoots of Picea abies, Larix decidua, Pseudotsuga menziesii, and Pinus nigra harvested from Romania and evaluate their antimicrobial and anti-virulence activity, as well as potential synergies with currently used antibiotics. The samples’ EO average content varied between 0.62% and 1.02% (mL/100 g plant). The mono- and sesquiterpene hydrocarbons were dominant in the composition of the studied EOs. The antimicrobial activity revealed that the minimum inhibitory concentration (MIC) values for the tested EOs and some pure compounds known for their antimicrobial activity ranged from 6.25 to 100 µL/mL. The most intensive antimicrobial effect was obtained for the Pinus nigra EO, which exhibited the best synergistic effect with some antibiotics against Staphylococcus aureus strains (i.e., oxacillin, tetracycline, erythromycin and gentamycin). The subinhibitory concentrations (sMIC) of the coniferous EOs inhibited the expression of soluble virulence factors (DN-ase, lipase, lecithinase, hemolysins, caseinase and siderophore-like), their efficiency being similar to that of the tested pure compounds, and inhibited the rhl gene expression in Pseudomonas aeruginosa, suggesting their virulence-arresting drug potential.