TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.)

2007 ◽  
Vol 91 (3) ◽  
pp. 243-250 ◽  
Author(s):  
Buhara Yucesan ◽  
Arzu Ucar Turker ◽  
Ekrem Gurel
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Cichorium Intybus ◽  
Multiple Shoot Formation

scholarly journals High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

2015 ◽  
Vol 3 (2) ◽  
pp. 202-209 ◽  
Author(s):  
R. Shashi Kumar ◽  
V. Krishna ◽  
. Venkatesh
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Synergetic Effect ◽  
Multiple Shoot ◽  
Coconut Water ◽  
Morphological Variations ◽  
Musa Paradisiaca ◽  
Shoot Initiation

High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 %) was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l), TDZ (0.1 to 1.2 mg/l) and coconut water (0.1 to 0.9 ml/l) induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg), number of hands in a bunch, number of fingers in a hand, length of the finger (cm), girth of the finger (cm) and girth of the pseudostem (cm) exhibited by in vitro plants were higher than the in vivo plants.Int J Appl Sci Biotechnol, Vol 3(2): 202-209 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12536 

scholarly journals Plant Regeneration Through Multiple Shoot Formation in Blackgram

2013 ◽  
Vol 18 (2) ◽  
pp. 11-16
Author(s):  
SK Roy ◽  
MS Haque ◽  
MK Siddiqua
Keyword(s):  
High Frequency ◽  
Genetic Improvement ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Direct Shoot Regeneration ◽  
Culture Studies ◽  
Plantlet Formation ◽  
Shoot Initiation ◽  
Rooting Medium ◽  
Shoot Tip Explants

Blackgram (Vigna mungo L. Hepper, syn. Phaseolus mungo L.) is one of the four major pulse crops. So far, no reports of tissue culture studies aiming at genetic improvement of this crop are available in Bangladesh. The present study comprised of experiments for direct shoot regeneration and plantlet formation in blackgram. Shoot tip explants were cultured on different concentrations of BAP (1.0, 5.0 and 10.0 mg L-1) and Kinetin (1.0, 5.0 and 10.0 mg L-1). The highest percentage (85.00%) of multiple shoot initiation was observed in the MS medium supplemented with 5.0 mg L-1 kinetin. The regenerated shoots were transferred to rooting medium supplemented with different concentrations of NAA. A high frequency of 85% rooting was observed with 0.2 mg L-1 NAA. The rooted plants were transferred to pots for hardening.DOI: http://dx.doi.org/10.3329/pa.v18i2.17458 Progress. Agric. 18(2): 11 - 16, 2007

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