Somatic embryogenesis and plant regeneration from immature zygotic embryo cultures of mountain ash (Sorbus pohuashanensis)

2012 ◽  
Vol 109 (3) ◽  
pp. 547-556 ◽  
Author(s):  
Ling Yang ◽  
Yuhua Li ◽  
Hailong Shen
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Zygotic Embryo ◽  
Immature Zygotic Embryo ◽  
Embryo Cultures ◽  
Mountain Ash

scholarly journals Reproductive Characteristics of the Selected Cocoa (Theobroma cacao L.) Clones after Regenerated from the Somatic Embryogenesis Culture

2021 ◽  
Vol 18 (24) ◽  
pp. 1406
Author(s):  
Gibson Entuni ◽  
Hollena Nori ◽  
Rebicca Edward ◽  
Ahmad Kamil bin Mohammad Jaafar
Keyword(s):  
Somatic Embryogenesis ◽  
Reproductive Cycle ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Reproductive Organ ◽  
Immature Zygotic Embryo ◽  
Reproductive Characteristics ◽  
Zygotic Embryo Culture ◽  
Typical Period ◽  
Immature Zygotic Embryo Culture

This study was conducted to evaluate the reproductive characteristics of 4 elite cocoa clones (MCBC1, PBC230, KKM22 and KKM4) propagated via somatic embryogenesis culture. From the findings, all clones have similar reproductive characteristics with clones from conventional grafted. However, only KKM4 clone from immature zygotic embryo culture produced the shortest staminode to style distance of 1.83 mm. This consequently influenced flower stability by reducing the efficiency of pollination by insects. It was found that this clone also has the highest number of flowers drop after anthesis (5 flowers) and lowest production of cherelle (5 cherelles). Further observation revealed that floral development from first bud visible (BBCH51) to flower anthesis (BBCH68) of all clones took around 31 days. These cocoa flowers which remained receptive soon after anthesis at 10 am (day-31) until the next day (day-32) suggesting 2 days’ period of receptivity. HIGHLIGHTS It is crucial to assess the presence of off-type characteristics in the reproductive organ structure such as the distance between staminode to style, period of reproductive cycle and stigmatic receptivity of cocoa clones regenerated from somatic embryogenesis The converging and parallel type of staminode to style distances are the ideal flower spatial arrangements for the optimal pollination in cocoa plant compared to splay type Only KKM4 clone propagated from immature zygotic embryo culture showed variation in the distance between staminode to style distance and this caused pollination failure by insect which then consequently caused minimum cherelle production All regenerated cocoa clones observed with typical period of the reproductive cycle and stigmatic receptivity GRAPHICAL ABSTRACT

Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum

1992 ◽  
Vol 11-11 (5-6) ◽  
Author(s):  
SusanH. Woods ◽  
GregoryC. Phillips ◽  
JohnE. Woods ◽  
GlennB. Collins
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Zygotic Embryo

scholarly journals Somatic Embryogenesis and Subsequent Plant Regeneration from Zygotic Embryo Derived Callus of Rubber (Hevea brasiliensis Muell. Arg).

2017 ◽  
Vol 27 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Md Mahbubur Rahman ◽  
Maziah Mahmood ◽  
Norhani Abdullah ◽  
Noor Azmi Shaharuddin ◽  
Waheeda Parvin
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Growth Regulators ◽  
Hevea Brasiliensis ◽  
Zygotic Embryo ◽  
Rubber Tree ◽  
Mass Propagation ◽  
Half Strength ◽  
First Time

A protocol has been developed for induction, maturation and germination of the zygotic embryo derived callus of the rubber tree (Hevea brasiliensis Muell. Arg.). The influence of plant growth regulators (PGRs) including 2,4‐D, α‐NAA, picloram, GA3 and TDZ on MS and MMS were studied. Optimum calli were induced on MS supplemented with 2.0 mg/l 2, 4‐D. The best callus growth and proliferation was recorded on MS fortified with 2.0 mg/l 2, 4‐D + 2.0 mg/l BAP + 0.5 mg/l NAA. The maximum embryonic calli were induced on MS + 2.0 mg/l 2, 4‐D + 2.0 mg/l Kn medium. Embryo induction, differentiation and maturation were obtained on MMS (MS +Vit B5). The rooted plantlets were produced on half strength MS without any supplements.The novelty of this study is the induction of embryos and plant regeneration from zygotic embryo explants of Hevea for the first time. The protocol developed in this study will facilitate mass propagation of high yielding rubber clones as well as to develop transgenic rubber plants with desired genes through genetic transformation.Plant Tissue Cult. & Biotech. 27(1): 51-61, 2017 (June)

Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of Areca catechu L. (Arecaceae)

2003 ◽  
Vol 39 (1) ◽  
pp. 34-36 ◽  
Author(s):  
Hsiang-Chih Wang ◽  
Jen-Tsung Chen ◽  
Shieh-Ping Wu ◽  
Mei-Chun Lin ◽  
Wei-Chin Chang
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Zygotic Embryo ◽  
Areca Catechu
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