Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum

1992 ◽  
Vol 11-11 (5-6) ◽  
Author(s):  
SusanH. Woods ◽  
GregoryC. Phillips ◽  
JohnE. Woods ◽  
GlennB. Collins
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Zygotic Embryo

scholarly journals Somatic Embryogenesis and Subsequent Plant Regeneration from Zygotic Embryo Derived Callus of Rubber (Hevea brasiliensis Muell. Arg).

2017 ◽  
Vol 27 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Md Mahbubur Rahman ◽  
Maziah Mahmood ◽  
Norhani Abdullah ◽  
Noor Azmi Shaharuddin ◽  
Waheeda Parvin
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Growth Regulators ◽  
Hevea Brasiliensis ◽  
Zygotic Embryo ◽  
Rubber Tree ◽  
Mass Propagation ◽  
Half Strength ◽  
First Time

A protocol has been developed for induction, maturation and germination of the zygotic embryo derived callus of the rubber tree (Hevea brasiliensis Muell. Arg.). The influence of plant growth regulators (PGRs) including 2,4‐D, α‐NAA, picloram, GA3 and TDZ on MS and MMS were studied. Optimum calli were induced on MS supplemented with 2.0 mg/l 2, 4‐D. The best callus growth and proliferation was recorded on MS fortified with 2.0 mg/l 2, 4‐D + 2.0 mg/l BAP + 0.5 mg/l NAA. The maximum embryonic calli were induced on MS + 2.0 mg/l 2, 4‐D + 2.0 mg/l Kn medium. Embryo induction, differentiation and maturation were obtained on MMS (MS +Vit B5). The rooted plantlets were produced on half strength MS without any supplements.The novelty of this study is the induction of embryos and plant regeneration from zygotic embryo explants of Hevea for the first time. The protocol developed in this study will facilitate mass propagation of high yielding rubber clones as well as to develop transgenic rubber plants with desired genes through genetic transformation.Plant Tissue Cult. & Biotech. 27(1): 51-61, 2017 (June)

Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of Areca catechu L. (Arecaceae)

2003 ◽  
Vol 39 (1) ◽  
pp. 34-36 ◽  
Author(s):  
Hsiang-Chih Wang ◽  
Jen-Tsung Chen ◽  
Shieh-Ping Wu ◽  
Mei-Chun Lin ◽  
Wei-Chin Chang
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Zygotic Embryo ◽  
Areca Catechu

High-frequency plant regeneration from immature zygotic embryo cultures of Houttuynia cordata Thunb via somatic embryogenesis

2013 ◽  
Vol 7 (4) ◽  
pp. 527-534 ◽  
Author(s):  
Myung Jin Oh ◽  
Myung Suk Ahn ◽  
Eun Yee Jie ◽  
Jang Ryol Liu ◽  
Byung Whan Min ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
High Frequency ◽  
Zygotic Embryo ◽  
Immature Zygotic Embryo ◽  
Houttuynia Cordata ◽  
Embryo Cultures ◽  
Houttuynia Cordata Thunb

Somatic embryogenesis and plant regeneration in immature zygotic embryo, ovule, and anther filament cultures of Chinese cabbage

1998 ◽  
Vol 72 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Pil S Choi ◽  
Sung R Min ◽  
Min Y Ahn ◽  
Woong Y Soh ◽  
Jang R Liu
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Chinese Cabbage ◽  
Zygotic Embryo ◽  
Immature Zygotic Embryo

scholarly journals Plant regeneration of southern Adriatic iris by somatic embryogenesis

2009 ◽  
Vol 61 (3) ◽  
pp. 413-418 ◽  
Author(s):  
Sladjana Jevremovic ◽  
Angelina Subotic ◽  
Milana Trifunovic ◽  
Marija Nikolic
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Balkan Peninsula ◽  
Embryogenic Calli ◽  
Subsequent Decrease ◽  
Simple Protocol ◽  
The Media ◽  
Dichlorophenoxy Acetic Acid

A simple protocol has been developed for plant regeneration by somatic embryogenesis of Southern Adriatic iris (Iris pseudopallida Trinajstic), an endemic species of the Balkan Peninsula. Somatic embryogenesis was induced in zygotic embryo culture on media supplemented with 2,4-dichlorophenoxy acetic acid (2-10 mgL-1) as the sole plant growth regulator, where both embryogenic calli and somatic embryos were induced. Subsequent decrease of 2,4-D in the media promoted formation of somatic embryos. Developed somatic embryos germinated on medium without growth regulators. The regenerated plantlets had diploid chromosome number. Planted plantlets acclimatized very well under greenhouse and garden conditions.

Plant regeneration by somatic embryogenesis in Pinus thunbergii resistant to the pine wood nematode

2019 ◽  
Vol 49 (12) ◽  
pp. 1604-1612
Author(s):  
Tingyu Sun ◽  
Yanli Wang ◽  
Lihua Zhu ◽  
Xiaoqin Wu ◽  
Jianren Ye
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryo ◽  
Cell Line ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Pinus Thunbergii ◽  
Embryogenic Tissue ◽  
Embryo Production ◽  
Somatic Embryo Production

Pine wilt disease (PWD) is a severe threat to pine forests in East Asia. Screening and breeding of resistant varieties is a very effective way to prevent and control PWD; however, no reliable somatic embryogenesis system has yet been developed for the elite nematode-resistant Pinus thunbergii Parl. line. In this study, we studied the plant regeneration via somatic embryogenesis of nematode-resistant P. thunbergii. Initiation of embryogenic tissue was significantly affected by seed family (p = 0.017), immature zygotic embryo stage (p = 0.032), and initiation medium (p = 0.004). Seed family 37 was the most favorable female parent for initiation of P. thunbergii. Furthermore, the initiation rate increased from the pre-embryonic stage to the cleavage polyembryonic stage. The optimal medium was I2, containing 2,4-dichlorophenoxyacetic acid (9 μmol·L−1) and 6-benzyladenine (4.4 μmol·L−1). A statistically significant interaction between cell line and subculture time (24 months) was observed in the influence on proliferation rate, somatic embryo production, and percentage germination (p < 0.001). In this study, the highest somatic embryo production was achieved using cell line 37-1 (1983 somatic embryos per gram fresh mass), with approximately 83.5% of somatic embryos germinating after transferring to germination medium, of which 77.6% converted into plantlets.

Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose

2009 ◽  
Vol 3 (3) ◽  
pp. 199-203 ◽  
Author(s):  
Suk Weon Kim ◽  
Myung Jin Oh ◽  
Jang R. Liu
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Zygotic Embryo ◽  
Explant Cultures
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