In vitro shoots from root explant, their encapsulation, storage, plant recovery and genetic fidelity assessment of Limonium hybrid ‘Misty Blue’: a florist plant

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. The objective of the present study is to determine the effect of polyamines (putrescine "PUT" and spermidine" SPD") and silver thiosulfate (STS) on enhancing propagation of date palm cv Quntar in vitro. Media supplemented with 75 mg L-1 SPD in combination with 10 mgL-1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root regeneration and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL-1 PUT and 10 mgL-1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

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Micropropagation, genetic fidelity assessment and phytochemical studies of Clerodendrum thomsoniae Balf. f. with special reference to its anti-stress properties

Research in Plant Biology ◽

10.25081/ripb.2019.v9.3779 ◽

2019 ◽

pp. 09-15 ◽

Cited By ~ 3

Author(s):

Pallab Kar ◽

Arnab Kumar Chakraborty ◽

Malay Bhattacharya ◽

Tanmayee Mishra ◽

Arnab Sen

Keyword(s):

Special Reference ◽

Genetic Fidelity ◽

Fidelity Assessment ◽

Callus Regeneration ◽

Murashige And Skoog's Medium ◽

Murashige And Skoog’S Medium ◽

Phytochemical Studies ◽

New Crop ◽

Nodal Culture

Clerodendrum thomsoniae commonly known as bleeding heart vine or bag flower which is a good candidate for a new crop for the floriculture industry. In this study, in-vitro callus regeneration of C. thomsoniae through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 2 mg/l BAP and 0.5 mg/l

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The Effect of Polyamines and Silver Thiosulphate on Micropropagation of Date Palm Followed by Genetic Stability Assessment

10.21203/rs.3.rs-1175104/v1 ◽

2022 ◽

Author(s):

Ahmed M.W Al-Mayahi

Keyword(s):

Tissue Culture ◽

Genetic Stability ◽

Date Palm ◽

Genetic Fidelity ◽

Silver Thiosulphate ◽

Silver Thiosulfate ◽

Practical Applications ◽

Fidelity Assessment ◽

Soil Transfer

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. This study’s objective was to investigate the effect of the two types of polyamines (putrescine "PUT" and spermidine" SPD") in combination with silver thiosulfate (STS) on the growth and development and genetic stability of cultures of Quntar cultivar. Media supplemented with 75 mg L−1 SPD in combination with 10 mgL−1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root formation and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL−1 PUT and 10 mgL−1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

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The influential role of polyamines on the in vitro regeneration of pea (Pisum sativum L.) and genetic fidelity assessment by SCoT and RAPD markers

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-019-01699-z ◽

2019 ◽

Vol 139 (3) ◽

pp. 547-561 ◽

Cited By ~ 2

Author(s):

Chandrasekaran Ajithan ◽

Venkatachalam Vasudevan ◽

Dorairaj Sathish ◽

Selvam Sathish ◽

Veda Krishnan ◽

...

Keyword(s):

Pisum Sativum ◽

Rapd Markers ◽

In Vitro Regeneration ◽

Genetic Fidelity ◽

Pisum Sativum L ◽

Fidelity Assessment

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Efficient in vitro plant regeneration from leaf derived callus and genetic fidelity assessment of an endemic medicinal plant of Ranunculus wallichianus Wight & Arnn using RAPD and ISSR markers

10.21203/rs.3.rs-432950/v1 ◽

2021 ◽

Author(s):

Srinivasan P ◽

David Raja H ◽

Tamilvanan R

Keyword(s):

Callus Formation ◽

Leaf Explants ◽

Genetic Fidelity ◽

Issr Markers ◽

Ms Medium ◽

Genetic Purity ◽

Fidelity Assessment ◽

Half Strength ◽

Rapd And Issr

Abstract Ranunculus wallichianus is a medicinally important plant and an endemic species to Western Ghats of South India. An efficient and reliable indirect regeneration protocol system for R. wallichianus was developed from leaf explants in the present investigation. Leaf explants were cultured on both full-strength and half-strength MS (Murashige & Skoog) medium supplemented with different concentrations (1.0 mg L− 1 to 3.0 mg L− 1) of 2,4-D and NAA. Among the different concentrations tested, the highest percentage of yellowish green compact nodular callus formation was observed on half-strength MS medium with 2.0 mg L− 1 of 2, 4-D. Then, the in vitro raised organogenic callus was cultured on half strength MS medium containing various concentrations (1.0 mg L− 1 to 3.0 mg L− 1) of BA, KIN and TDZ with 0.5 mg L− 1 NAA and 10% CW for in vitro shoot regeneration. The highest percentage of regeneration response (97%) and maximum number of shoots formation (11.1 ± 0.13 shoots/culture with 9.2 ± 0.35 cm mean shoot length) were obtained from MS medium containing 2.5 mg L− 1 BA with 0.5 mg L− 1 NAA and 10% CW. The well elongated in vitro raised shoots were rooted in half strength MS medium with 2.5 mg L− 1 IBA + 250 mg L− 1 activated charcoal shows high frequency of root formation. The well rooted plantlets were successfully hardened and acclimatized with the survival rate of 94%. Clonal fidelity of in vitro raised plantlets was assessed by using DNA based RAPD and ISSR molecular markers. The total of 56 and 47 monomorphic bands were obtained from RAPD and ISSR markers respectively. This present in vitro propagation protocol system could be an effective for the conservation of R. wallichianus with their genetic purity and its further investigations.

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