The influential role of polyamines on the in vitro regeneration of pea (Pisum sativum L.) and genetic fidelity assessment by SCoT and RAPD markers

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

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An efficient in vitro regeneration system of fieldpea (Pisum sativum L.) via. shoot organogenesis

Journal of Plant Biochemistry and Biotechnology ◽

10.1007/s13562-013-0200-3 ◽

2013 ◽

Vol 23 (2) ◽

pp. 184-189 ◽

Cited By ~ 5

Author(s):

Alok Das ◽

Sumit Kumar ◽

P. Nandeesha ◽

Indu Singh Yadav ◽

Jyoti Saini ◽

...

Keyword(s):

Pisum Sativum ◽

Shoot Organogenesis ◽

In Vitro Regeneration ◽

Regeneration System ◽

Pisum Sativum L

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In vitro differentiation and plant regeneration from root and other explants of juvenile origin in pea (Pisum sativum L.)

Legume Research - An International Journal ◽

10.18805/lr.v0iof.9097 ◽

2017 ◽

Cited By ~ 1

Author(s):

Shikha Sharma ◽

Geetika Gambhir ◽

D. K. Srivastava

Keyword(s):

Pisum Sativum ◽

Shoot Regeneration ◽

In Vitro Regeneration ◽

Leaf Explants ◽

Cotyledonary Node ◽

Shoot Elongation ◽

Root Regeneration ◽

Pisum Sativum L ◽

Regenerated Plantlets

In vitro regeneration of pea explants (Pisum sativum L. var. ‘Lincon’) was done in 49 different combinations and concentrations of BAP, BAP and NAA, BAP and IBA, TDZ, TDZ and Adenine for shoot regeneration from hypocotyl, root, leaf and cotyledonary node. High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively. No shoot regeneration was obtained from leaf explants on any of the combination used. Shoot elongation was observed on the same medium used for shoot regeneration respectively.MS medium supplemented with 0.20 mg/l IBA was found best for root regeneration from in vitro raised shoots. The plantlets were able to regenerate within 6-7 weeks. The regenerated plantlets were acclimatized in pre-sterilized cocopeat.

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Pea (Pisum sativum L.) in biology prior and after Mendel's discovery

Czech Journal of Genetics and Plant Breeding ◽

10.17221/2/2014-cjgpb ◽

2014 ◽

Vol 50 (No. 2) ◽

pp. 52-64 ◽

Cited By ~ 11

Author(s):

P. Smýkal

Keyword(s):

Pisum Sativum ◽

Reverse Genetics ◽

Agronomic Traits ◽

In Vitro Regeneration ◽

Lotus Japonicus ◽

Model Organism ◽

Germplasm Collections ◽

Economic Significance ◽

Pisum Sativum L

Pea (Pisum sativum L.) has been extensively used in early hybridization studies and it was the model organism of choice for Mendel’s discovery of the laws of inheritance, making pea part of the foundation of modern genetics. Pea has also been used as model for experimental morphology and physiology. However, subsequent progress in pea genomics has lagged behind many other plant species, largely as a consequence of its genome size and low economic significance. The availability of the genome sequences of five legume species (Medicago truncatula, Lotus japonicus, Glycine max, Cajanus cajan and Cicer aerietinum) offers opportunities for genome wide comparison. The combination of a candidate gene and synteny approach has allowed the identification of genes underlying agronomically important traits such as virus resistances and plant architecture. Useful genomic resources already exist and include several types of molecular marker sets as well as both transcriptome and proteome datasets. The advent of greater computational power and access to diverse germplasm collections enable the use of association mapping to identify genetic variation related to desirable agronomic traits. Current genomic knowledge and technologies can facilitate the allele mining for novel traits and their incorporation from wild Pisum sp. into elite domestic backgrounds. Fast neutron and targeting-induced local lesions in genomes (TILLING) pea mutant populations are available for reverse genetics approaches, BAC libraries for positional gene cloning as well as transgenic and in vitro regeneration for proof of function through gene silencing or over-expression. Finally, recently formed International Pea Genome Sequencing Consortium, holds promise to provide the pea genome sequence by 2015, a year of 150 anniversary of Mendel’s work.

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