Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions

The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in the genus Trichosporon. Comparative sequence analysis suggests that six medically relevant species, T. asahii, T. asteroides, T. cutaneum,T. inkin, T. mucoides, and T. ovoides, can be readily identified by their ITS sequences. In addition, the sequence analysis showed that conspecific strains have fewer than 1% nucleotide differences in the ITS 1 and 2 regions overall. Molecular phylogenetic trees are also presented.

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Genetic Diversity of Yeasts from Fermented Orange Juice Based on PCR-RFLP and Sequence Analysis of the Internal Transcribed Spacer Regions

Microbiology Indonesia ◽

10.5454/mi.4.1.1 ◽

2010 ◽

Vol 4 (1) ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

SUSAN SOKA ◽

ANASTASIA SUSANTO

Keyword(s):

Genetic Diversity ◽

Sequence Analysis ◽

Orange Juice ◽

Internal Transcribed Spacer ◽

Internal Transcribed Spacer Regions ◽

Pcr Rflp

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Molecular characterization of Mycobacterium intracellulare-related strains based on the sequence analysis of hsp65, internal transcribed spacer and 16S rRNA genes

Journal of Medical Microbiology ◽

10.1099/jmm.0.020727-0 ◽

2010 ◽

Vol 59 (9) ◽

pp. 1037-1043 ◽

Cited By ~ 18

Author(s):

Joo-Hee Park ◽

Tae-Sun Shim ◽

Seung-Ae Lee ◽

Hyungki Lee ◽

In-Kyung Lee ◽

...

Keyword(s):

Sequence Analysis ◽

16S Rrna ◽

Internal Transcribed Spacer ◽

16S Rrna Genes ◽

Rrna Genes ◽

Rrna Gene ◽

Mycobacterium Intracellulare ◽

Epidemiological Features ◽

The 16S Rrna Gene

We investigated the molecular epidemiological features of 94 Mycobacterium intracellulare-related strains, isolated from Korean patients, using sequence analysis targeting 3 independent chronometer molecules, hsp65, the internal transcribed spacer 1 region and the 16S rRNA gene. By collective consideration of these three gene-based approaches, the 94 strains were divided into 5 groups (INT1, INT2, INT3, INT4 and INT5). The frequencies of genotype INT1, 2, 3, 4 and 5 in the 94 isolates were 57.4 % (54), 27.7 % (26), 6.4 % (6), 5.3 % (5) and 3.2 % (3), respectively. When correlations between genotypes and clinical parameters (age, sex, radiological type and the presence of a cavity) were analysed in 78 patients with non-tuberculous mycobacteria pulmonary diseases, no relationships were observed with respect to age, sex and radiological type, but genotype and the presence of a cavity tended to be related (P=0.051).

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Characterization of Metarhizium anisopliae using amplifed ribossomal DNA restriction analysis (ARDRA) and internal transcribed spacer (ITS) sequence analysis

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb12.906 ◽

2012 ◽

Vol 11 (101) ◽

pp. 16635-16639

Author(s):

Maria do Carmo Catanho Pereira de Lyra ◽

◽

Maria Luiza Ribeiro Bastos da Silva ◽

Vanildo Alberto Leal Bezerra Cavalcanti ◽

Adália Cavalcanti Espírito Santo Mergulhão

Keyword(s):

Sequence Analysis ◽

Metarhizium Anisopliae ◽

Internal Transcribed Spacer ◽

Restriction Analysis ◽

Its Sequence ◽

Its Sequence Analysis ◽

Dna Restriction

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CloVR-ITS: Automated internal transcribed spacer amplicon sequence analysis pipeline for the characterization of fungal microbiota

Microbiome ◽

10.1186/2049-2618-1-6 ◽

2013 ◽

Vol 1 (1) ◽

Cited By ~ 26

Author(s):

James Robert White ◽

Cynthia Maddox ◽

Owen White ◽

Samuel V Angiuoli ◽

W Florian Fricke

Keyword(s):

Sequence Analysis ◽

Internal Transcribed Spacer ◽

Analysis Pipeline ◽

Fungal Microbiota

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Comparison of the 5.8S rRNA Gene and Internal Transcribed Spacer Regions of Trichomonadid Protozoa Recovered from the Bovine Preputial Cavity

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870301500104 ◽

2003 ◽

Vol 15 (1) ◽

pp. 14-20 ◽

Cited By ~ 42

Author(s):

R. L. Walker ◽

D. C. Hayes ◽

S. J. Sawyer ◽

R. W. Nordhausen ◽

K. A. Van Hoosear ◽

...

Keyword(s):

Sequence Analysis ◽

Internal Transcribed Spacer ◽

Rrna Gene ◽

Tritrichomonas Foetus ◽

Similarity Matrix ◽

Multiple Sequence ◽

Internal Transcribed Spacer Regions ◽

5.8S Rrna Gene ◽

5.8S Rrna ◽

High Degree

Sequence analysis of the 5.8S rRNA gene and the internal transcribed spacer regions (ITSRs) was used to compare trichomonadid protozoa ( n = 39) of varying morphologies isolated from the bovine preputial cavity. A multiple sequence alignment was performed with bovine isolate sequences and other trichomonadid protozoa sequences available in GenBank. As a group, Tritrichomonas foetus isolates ( n = 7) had nearly complete homology. A similarity matrix showed low homology between the T. foetus isolates and other trichomonads recovered from cattle (<70%). Two clusters of trichomonads other than T. foetus were identified. Eighteen isolates comprised 1 group. These isolates shared >99% homology among themselves and with Pen-tatrichomonas hominis. The other non– T. foetus cluster ( n = 14) did not exhibit a high degree of homology (<87%) with other bovine isolates or any of the trichomonad sequences available in GenBank. The sequence homology among isolates in that cluster was >99%, except for 1 isolate that varied from the others in both ITSRs (∼2% dissimilarity). Sequence analysis of the 5.8S rRNA gene and ITSRs was useful for comparing trichomonadid protozoa isolated from the bovine preputial cavity and demonstrated that 2 distinct types of trichomonads constituted the non– T. foetus isolates recovered from the bovine preputial cavity.

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