scholarly journals Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions

2007 ◽  
Vol 45 (3) ◽  
pp. 1079-1079
Author(s):  
S. N. Leaw ◽  
H. C. Chang ◽  
H. F. Sun ◽  
R. Barton ◽  
J.-P. Bouchara ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Yeast Species ◽  
Internal Transcribed Spacer Regions

scholarly journals Sequence analysis of the ribosomal DNA internal transcribed spacer regions in Termitomyces heimii species

2011 ◽  
Vol 62 (2) ◽  
pp. 797-803 ◽  
Author(s):  
Shafiquzzaman Siddiquee ◽  
Weng Yan Yee ◽  
Khanam Taslima ◽  
Nur Hasan Nudin Fatihah ◽  
S. Vijay Kumar ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Regions

Sequence Analysis of the Internal Transcribed Spacer 2 (ITS2) from Yeast Species Within the Genus Candida

1998 ◽  
Vol 36 (2) ◽  
pp. 63-69 ◽  
Author(s):  
Timothy J. Lott ◽  
Brian M. Burns ◽  
Rosely Zancope-Oliveira ◽  
Cheryl M. Elie ◽  
Errol Reiss
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Yeast Species ◽  
Internal Transcribed Spacer 2

scholarly journals Identification of Medically RelevantTrichosporon Species Based on Sequences of Internal Transcribed Spacer Regions and Construction of a Database forTrichosporon Identification

1999 ◽  
Vol 37 (6) ◽  
pp. 1985-1993 ◽  
Author(s):  
Takashi Sugita ◽  
Akemi Nishikawa ◽  
Reiko Ikeda ◽  
Takako Shinoda
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Comparative Sequence Analysis ◽  
Nucleotide Sequences ◽  
Its Sequences ◽  
Rrna Gene ◽  
Comparative Sequence ◽  
Internal Transcribed Spacer Regions ◽  
Molecular Phylogenetic

The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in the genus Trichosporon. Comparative sequence analysis suggests that six medically relevant species, T. asahii, T. asteroides, T. cutaneum,T. inkin, T. mucoides, and T. ovoides, can be readily identified by their ITS sequences. In addition, the sequence analysis showed that conspecific strains have fewer than 1% nucleotide differences in the ITS 1 and 2 regions overall. Molecular phylogenetic trees are also presented.

scholarly journals Internal transcribed spacer for identification of yeast species isolated from cancer patients at the Isotope and Radiation Center, Khartoum, Sudan: A cross-sectional, case-control study

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 443 ◽  
Author(s):  
Mohamed M.A. Nagla ◽  
Omer E. El Fadil ◽  
Abdel Hamid M. Muzamil ◽  
Altayeb N. Hisham ◽  
Mohamed B. Bahaeldeen ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Cancer Patients ◽  
Internal Transcribed Spacer ◽  
Yeast Species ◽  
Reference Sequence ◽  
Positive Sequence ◽  
Its Sequence ◽  
Control Groups ◽  
Its Sequence Analysis ◽  
And Control

Background:Cancer patients have a high risk of fungal infections, especially by Candida species.Non-C. albicans Candidainfections and less common yeast infections have been increasing in recent years. Identification by conventional methods can be difficult and sometimes inconclusive. This study aimed to detect the prevalence of oral yeast species isolated from cancer patients, from oral swab, sputum and urine, using Internal Transcribed Spacer (ITS) sequence analysis, since little is known about this problem in Sudan.Methods:The study involved 333 cancer patients (168 patients under treatment [study group] and 165 patients before treatment [control group]). Oral swabs were collected from all patients. Urine or sputum specimens were collected from patients under treatment showing clinical features of UTI or lower respiratory tract infection, respectively. ITS1 and ITS2 region of  isolated yeast were amplified by PCR and sequenced. The obtained sequences were compared to reference sequence available in the GenBank database using BLAST.Results:Culture results showed oral yeastspecies were isolated from 69/168 (41.1%) and 74/165 (44.8%) of patients among study and control groups, respectively (P value > 0.05). 2/9 (22.2%) patients were urine growth positive and 8/14 (57.1%) patients were sputum culture positive. Sequence analysis showed,C. albicanswas the most prevalent organism (93; 52.5%) followed byC. tropicalis(29; 16.4%), andC. glabrata(24; 13.6%). Non-C. albicans Candidaand uncommon rare yeast were found to be associated with oral infections and colonization among the study and control groups, whereasC.albicans was the most common species  (66.7%) associated with oral candidiasis among  the treated patients.         Conclusion:Cancer patients were highly colonized with different oral yeast species, which indicates that ITS sequence analysis is an accurate method for identification. This will aid effective management to prevent dissemination of disease especially among those who are under chemo and/or radiotherapy treatment.

scholarly journals Comparison of the 5.8S rRNA Gene and Internal Transcribed Spacer Regions of Trichomonadid Protozoa Recovered from the Bovine Preputial Cavity

2003 ◽  
Vol 15 (1) ◽  
pp. 14-20 ◽  
Author(s):  
R. L. Walker ◽  
D. C. Hayes ◽  
S. J. Sawyer ◽  
R. W. Nordhausen ◽  
K. A. Van Hoosear ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Rrna Gene ◽  
Tritrichomonas Foetus ◽  
Similarity Matrix ◽  
Multiple Sequence ◽  
Internal Transcribed Spacer Regions ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
High Degree

Sequence analysis of the 5.8S rRNA gene and the internal transcribed spacer regions (ITSRs) was used to compare trichomonadid protozoa ( n = 39) of varying morphologies isolated from the bovine preputial cavity. A multiple sequence alignment was performed with bovine isolate sequences and other trichomonadid protozoa sequences available in GenBank. As a group, Tritrichomonas foetus isolates ( n = 7) had nearly complete homology. A similarity matrix showed low homology between the T. foetus isolates and other trichomonads recovered from cattle (<70%). Two clusters of trichomonads other than T. foetus were identified. Eighteen isolates comprised 1 group. These isolates shared >99% homology among themselves and with Pen-tatrichomonas hominis. The other non– T. foetus cluster ( n = 14) did not exhibit a high degree of homology (<87%) with other bovine isolates or any of the trichomonad sequences available in GenBank. The sequence homology among isolates in that cluster was >99%, except for 1 isolate that varied from the others in both ITSRs (∼2% dissimilarity). Sequence analysis of the 5.8S rRNA gene and ITSRs was useful for comparing trichomonadid protozoa isolated from the bovine preputial cavity and demonstrated that 2 distinct types of trichomonads constituted the non– T. foetus isolates recovered from the bovine preputial cavity.

Wickerhamomyces xylosica sp. nov. and Candida phayaonensis sp. nov., two xylose-assimilating yeast species from soil

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2786-2792 ◽  
Author(s):  
Savitree Limtong ◽  
Sukanya Nitiyon ◽  
Rungluk Kaewwichian ◽  
Sasitorn Jindamorakot ◽  
Somjit Am-In ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
D2 Domain ◽  
Ascomycete Yeast ◽  
Large Subunit Rrna

Two strains (NT29T and NT31T) of xylose-assimilating yeasts were obtained from soils collected in northern Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were found to represent two novel ascomycete yeast species. Strain NT29T was assigned to the genus Candida belonging to the Pichia clade as a representative of Candida phayaonensis sp. nov.; the type strain is NT29T ( = BCC 47634T = NBRC 108868T = CBS 12319T). Strain NT31T represented a novel Wickerhamomyces species, which was named Wickerhamomyces xylosica sp. nov.; the type strain is NT31T ( = BCC 47635T = NBRC 108869T = CBS 12320T).

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