Characterization of Metarhizium anisopliae using amplifed ribossomal DNA restriction analysis (ARDRA) and internal transcribed spacer (ITS) sequence analysis

2012 ◽  
Vol 11 (101) ◽  
pp. 16635-16639
Author(s):  
Maria do Carmo Catanho Pereira de Lyra ◽  
◽  
Maria Luiza Ribeiro Bastos da Silva ◽  
Vanildo Alberto Leal Bezerra Cavalcanti ◽  
Adália Cavalcanti Espírito Santo Mergulhão
Keyword(s):  
Sequence Analysis ◽  
Metarhizium Anisopliae ◽  
Internal Transcribed Spacer ◽  
Restriction Analysis ◽  
Its Sequence ◽  
Its Sequence Analysis ◽  
Dna Restriction

scholarly journals WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Victor Olusegun Oyetayo
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Its Sequence ◽  
African Countries ◽  
African Species ◽  
Its Sequence Analysis ◽  
Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

scholarly journals Internal transcribed spacer for identification of yeast species isolated from cancer patients at the Isotope and Radiation Center, Khartoum, Sudan: A cross-sectional, case-control study

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 443 ◽  
Author(s):  
Mohamed M.A. Nagla ◽  
Omer E. El Fadil ◽  
Abdel Hamid M. Muzamil ◽  
Altayeb N. Hisham ◽  
Mohamed B. Bahaeldeen ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Cancer Patients ◽  
Internal Transcribed Spacer ◽  
Yeast Species ◽  
Reference Sequence ◽  
Positive Sequence ◽  
Its Sequence ◽  
Control Groups ◽  
Its Sequence Analysis ◽  
And Control

Background:Cancer patients have a high risk of fungal infections, especially by Candida species.Non-C. albicans Candidainfections and less common yeast infections have been increasing in recent years. Identification by conventional methods can be difficult and sometimes inconclusive. This study aimed to detect the prevalence of oral yeast species isolated from cancer patients, from oral swab, sputum and urine, using Internal Transcribed Spacer (ITS) sequence analysis, since little is known about this problem in Sudan.Methods:The study involved 333 cancer patients (168 patients under treatment [study group] and 165 patients before treatment [control group]). Oral swabs were collected from all patients. Urine or sputum specimens were collected from patients under treatment showing clinical features of UTI or lower respiratory tract infection, respectively. ITS1 and ITS2 region of  isolated yeast were amplified by PCR and sequenced. The obtained sequences were compared to reference sequence available in the GenBank database using BLAST.Results:Culture results showed oral yeastspecies were isolated from 69/168 (41.1%) and 74/165 (44.8%) of patients among study and control groups, respectively (P value > 0.05). 2/9 (22.2%) patients were urine growth positive and 8/14 (57.1%) patients were sputum culture positive. Sequence analysis showed,C. albicanswas the most prevalent organism (93; 52.5%) followed byC. tropicalis(29; 16.4%), andC. glabrata(24; 13.6%). Non-C. albicans Candidaand uncommon rare yeast were found to be associated with oral infections and colonization among the study and control groups, whereasC.albicans was the most common species  (66.7%) associated with oral candidiasis among  the treated patients.         Conclusion:Cancer patients were highly colonized with different oral yeast species, which indicates that ITS sequence analysis is an accurate method for identification. This will aid effective management to prevent dissemination of disease especially among those who are under chemo and/or radiotherapy treatment.

Typing Tuber ectomycorrhizae by polymerase chain amplification of the internal transcribed spacer of rDNA and the sequence characterized amplified region markers

1997 ◽  
Vol 43 (8) ◽  
pp. 723-728 ◽  
Author(s):  
Delphine Gandeboeuf ◽  
Chantal Dupré ◽  
Gérard Chevalier ◽  
Paul Nicolas ◽  
Patricia Roeckel-Drevet
Keyword(s):  
Internal Transcribed Spacer ◽  
Restriction Analysis ◽  
Morphological Characteristics ◽  
Tuber Melanosporum ◽  
Sequence Characterized Amplified Region ◽  
Rdna Its ◽  
Its Sequence Analysis ◽  
Polymerase Chain ◽  
Tuber Species

Identification of some economically important Tuber species using classical morphological characteristics is sometimes difficult. We report here the molecular characterization of a species coming from China, Tuber indicum, mistaken with Tuber melanosporum species. Using restriction analysis of the amplified internal transcribed spacer (ITS) of rDNA, ITS sequence analysis, and sequence characterized amplified region markers, with DNA from fruit bodies or mycorrhizae, genetic variation was found between these two species, allowing to differentiate and characterize them.Key words: molecular identification, Tuber, internal transcribed spacer, sequence characterized amplified region.

Screening and Identification of β-Glucosidase Producing Strain of Fusarium Oxysporum

2013 ◽  
Vol 652-654 ◽  
pp. 402-405
Author(s):  
Jie Chen ◽  
De Fang Dai ◽  
Xiu Ling Gu ◽  
Lin Yuan
Keyword(s):  
Sequence Analysis ◽  
Fusarium Oxysporum ◽  
Internal Transcribed Spacer ◽  
Morphological Features ◽  
Its Sequence ◽  
Its Sequence Analysis ◽  
Screening And Identification

Five strains, which can produce β-glucosidase, were screened out from 96 strains isolated from linseed from different regions in China. Based on morphological features and internal transcribed spacer (ITS) sequence analysis, the five strains were all determined as Fusarium oxysporum. The strain named as S15 produces the highest β-glucosidase, which activity reach at 13.58 U/mL.

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