EXPERIMENTAL STUDY OF THE INFLUENCE OF TEMPERATURE ON PASTEURIZATION OF PÊRA RIO IN NATURA ORANGE JUICE

Heat treatment is one of the most used methods to preserve food, such as orange juices, which are an excellent source of ascorbic acid. To avoid vitamin C degradation and reduce loss, fast heating is recommended. This work aimed to determine the vitamin C content using the iodometric method and the convective heat transfer coefficient using the method of dimensionless numbers and the experimental method. Time and temperature were controlled throughout the experiment. In pasteurization, the solution was heated to 80 °C, heating lasted 50 minutes and cooling for 42 minutes. The convective heat transfer coefficient was evaluated in two regions of the cylindrical container: near the wall and in the central region. The graphic profile of the curve follows the same trend of the literature. The convective heat transfer coefficient is higher in the region near the wall. As time passes and temperature decreases, the central region tends to equilibrium, and the coefficient becomes more constant. The vitamin C content remained constant before and after pasteurization, so it was observed that the pasteurization did not cause ascorbic acid degradation since the heating step was fast in the heat treatment. As a result of the study, it was noted that studying the thermal behavior in the cooling of orange juice is extremely important to ensure its quality. It is pertinent to mention that in order to avoid this degradation and reduce its loss, it is necessary that in thermal treatments, fast heating is carried out and that the juice has low exposure to air and heat at the time of its preparation.

High Pressure Processing Impact on Emerging Mycotoxins (ENNA, ENNA1, ENNB, ENNB1) Mitigation in Different Juice and Juice-Milk Matrices

The aim of the present study was to investigate the potential of high-pressure processing (HPP) (600 MPa during 5 min) on emerging mycotoxins, enniatin A (ENNA), enniatin A1 (ENNA1), enniatin B (ENNB), enniatin B1 (ENNB1) reduction in different juice/milk models, and to compare it with the effect of a traditional thermal treatment (HT) (90 °C during 21 s). For this purpose, different juice models (orange juice, orange juice/milk beverage, strawberry juice, strawberry juice/milk beverage, grape juice and grape juice/milk beverage) were prepared and spiked individually with ENNA, ENNA1, ENNB and ENNB1 at a concentration of 100 µg/L. After HPP and HT treatments, ENNs were extracted from treated samples and controls employing dispersive liquid-liquid microextraction methodology (DLLME) and determined by liquid chromatography coupled to ion-trap tandem mass spectrometry (HPLC-MS/MS-IT). The results obtained revealed higher reduction percentages (11% to 75.4%) when the samples were treated under HPP technology. Thermal treatment allowed reduction percentages varying from 2.6% to 24.3%, at best, being ENNA1 the only enniatin that was reduced in all juice models. In general, no significant differences (p > 0.05) were observed when the reductions obtained for each enniatin were evaluated according to the kind of juice model, so no matrix effects were observed for most cases. HPP technology can constitute an effective tool in mycotoxins removal from juices.

Peningkatan Kualitas Dan Kuantitas Produk Umkm Sari Buah Jeruk Sebagai Minuman Imunostimulan Alami Untuk Menarik Daya Beli Masyarakat di Masa Pandemi

The COVID-19 pandemic is not over yet. One way to fight COVID-19 is to increase the body's immunity by consuming various vitamins which have function as an immunostimulant. One of them is vitamin C which is found in many fresh fruits. This opens up the opportunities for people in the informal sector to provide immunostimulants drinks. The Small Medium Enterprise (SME that produce fresh orange juice are considered prospective during this pandemic. However, in reality, their consumers has actually decreased, as experienced by SME Kishi Sari Jeruk Segar in the Surakarta City. This SME also faces several problems, i.e: seasonal stock of oranges so that the cost of supply of raw materials is unstable and juice drink products are not durable. Our community service team helped this SME in 2 aspects. In the production aspect, a refrigerator was provided to store raw fruit materials so that they do not damage quickly and to produce frozen juice. Meanwhile, in the marketing aspect, we designed a nutritional information facts information of the orange juice especially on immunostimulant content. This is expected to increase the MSE product attractiveness. Abstrak Saat ini pandemi COVID-19 belum berakhir. Salah satu cara melawan COVID-19 adalah meningkatkan imunitas tubuh dengan mengkonsumsi vitamin. Jenis vitamin yang terbukti berfungsi sebagai imunostimulan adalah vitamin C. Vitamin C banyak terkandung dalam buah segar seperti jeruk. Hal ini membuka peluang usaha bagi masyarakat di sektor informal untuk menyediakan imunostimulan dalam bentuk minuman. UMKM sari buah jeruk dipandang prospektif di masa pandemi ini. Namun kenyataanya jumlah pembeli produk UMKM sari buah jeruk justru mengalami penurunan seperti yang dialami oleh UMKM Kishi Sari Jeruk Segar di kota Surakarta. UMKM ini juga menghadapi beberapa permasalahan lain berupa stok bahan baku buah jeruk yang bersifat musiman sehingga cost supply bahan baku tidak stabil serta produk minuman sari buah jeruk yang bersifat tidak tahan lama. Usaha yang telah dilakukan oleh tim pengabdian kami meliputi 2 aspek. Pada aspek produksi diberikan almari es untuk menyimpan bahan baku buah agar tidak cepat busuk dan memproduksi sari buah jeruk frozen/beku. Solusi ini akan menjamin harga jual produk yang stabil serta produk yang memiliki daya tahan penyimpanan yang lebih panjang. Sedangkan pada aspek pemasaran dilakukan pencantuman informasi mengenai kandungan nutrisi yang berkhasiat sebagai imunostimulan. Hal ini dilakukan untuk meningkatkan daya tarik produk UMKM.

Influence of probiotics on physico-chemical and organoleptic characteristics of sweet orange juice

Aim: The study was undertaken to evaluate the survival probiotic organisms and its influence on the physical, chemical, nutritional and sensory characteristics of sweet orange juice. Methodology: Two samples of probiotic juice were prepared with 10 percent innoculum containing LAB strains (Lactobacillus bulgaricus and Lactobacillus plantarum). Sample A (without encapsulated strains) and Sample-B (with encapsulated strains) were prepared and incubated for 10hrs at 35oC. After incubation, the physico-chemical analysis of both the samples were analyzed for TSS, pH, acidity, total sugars, reducing sugars and ascorbic acid content. Results: The results of TSS, pH, acidity, total sugars, reducing sugars and ascorbic acid content for sample –A and Sample –B were 11.4˚Brix, 3.51, 0.82 percent, 6.1 percent, 1.5 percent, 4.6 percent, 40mgml-1 and 11.6˚ Brix, 3.68, 0.77 percent, 6.4 percent, 1.7 percent, 4.9 percent, 40 mg ml-1, respectively. Sensory evaluation revealed that overall acceptance of probiotic juice containing encapsulated strains and free strains in the first week was 8.3 and 7.8, respectively. Even after 4 weeks of storage, the overall acceptance for juice with encapsulated strains was better than free strains with a score of 7.5 and 7.0 at the end of storage period. Interpretation: The sweet orange juice with encapsulated strains has high viable cell count (109cfu ml-1) even after 4 weeks of storage resulted in stable therapeutic probiotic sweet orange juice. It is further, suitable for commercial production of probiotic sweet orange juice with probiotic cultures.

Development of Carrot Nutraceutical Products as an Alternative Supplement for the Prevention of Nutritional Diseases

Nutraceuticals can serve as an alternative supplement to overcome nutritional deficiency for a healthy lifestyle. They can also play a key role in disease management. To develop carrot nutraceutical products, 64 genotypes from four different continents were evaluated for a range of morpho-nutrition variables. Genetic variability, heritability, strength and direction of association among variables, and direct and indirect relationships among physiochemical and nutritional traits with β-carotene content were evaluated. Core diameter, foliage weight, root weight and shoulder weight showed significant association with β-carotene accumulation. Principal component analysis for physiochemical and nutritional assessment divided these genotypes into two distinctive groups, Eastern carrots and Western carrots. Caloric and moisture content had high positive associations with β-carotene content while carbohydrate content was negatively associated. Five genotypes (T-29, PI 634658, PI 288765, PI 164798, and Ames 25043) with the highest β-carotene contents were selected for making three nutraceutical supplements (carrot-orange juice, carrot jam and carrot candies). These nutraceutical supplements retained high β-carotene content coupled with antioxidant properties. Carrot jam (6.5 mg/100 g) and carrot candies (4.8 mg/100 g) had greater concentrations of β-carotene than carrot-orange juice (1.017 mg/100 g). Carrot jam presented high antioxidant activity with the highest values in T-29 (39% inhibition of oxidation) followed by PI 634658 (37%), PI 164798 (36.5%), Ames 25043 (36%) and PI 288765 (35.5%). These nutraceutical products, with 4–6.5 mg/100 g β-carotene content, had higher values than the USDA recommended dietary intake of 3–6 mg β-carotene/day can be recommended for daily use to lower the risk of chronic disease.

Development of functional caramel using system packages MathLab

The article presents substantiation for expanding the market for functional food products, in particular, confectionery - caramel. Proper nutrition is an integral part of a modern person diet; due to their properties, they increase the immunity and immune status of a person. The aim of the work is to create composite mixtures for sugar-free caramel using modern software. The studies were carried out using the MathLab software package, in particular the Statistica 10.0 software. A methodology for creating recipes using system packages has been developed. To enrich the caramel, the following components were selected: apple juice, coltsfoot extract, orange juice, mint and burdock extract, cranberry and lingonberry juices. An optimal recipe has been designed and equations are presented for calculating the dosages of the components of the enriched sugar-free caramel based on a natural sweetener isomalt with the addition of plant-based additives containing macro and micronutrients necessary for the normal functioning of the human body with a diagnosis of type II diabetes mellitus.

​Health-promoting Effects of Developed Probiotic Orange Beverage: An in vitro Study

Background: Probiotic food has evolved as the new trend among the health fanatics because of their proven benefits in preventing many diseases. With change in time the way of consuming probiotics has also changed. Unlike past dairy is not the only option for commercial probiotic production, recently fruit juices have become the popular choice for it. So the current study aimed to assess the feasibility of orange juice (Citrus reticulate) as a potential probiotic carrier for the production of probiotic orange juice with lactic acid bacteria. Methods: Three test samples (TS) were developed with different combination of lactic acid probiotic bacteria viz. test sample 1 (TS1) (L. bulgaricus and L. casei), TS2 (L. bulgaricus, L. casei and L. gasseri) and TS3 (L. bulgaricus, L. casei, L. gasseri and L. fermentum). The orange juice was pasteurized for 2 min at 90°C and was inoculated at a rate of 10% inoculum. All the test samples were fermented for 4 hrs at 37°C and the physicochemical and nutritional characteristics were evaluated along with their in vitro hypocholesterolemic and in vitro hypoglycemic efficacies. Result: The probiotic orange test samples did not show inferior properties than the control in terms of physicochemical and nutritional properties. The bacterial count was decreased with time but remained above standard limit (107cfu/100ml) until 28th day of refrigerated storage. All the test samples showed promising antioxidant activity, in vitro hypocholesterolemic activity and in vitro hypoglycemic activities. Hence orange juice could be used as a suitable probiotic carrier for production of novel probiotic beverages.

Effect of soft beverages on the in vitro dissolution of gastro-resistant tablets containing low dose Acetylsalicylic acid

The need for additional fluids for easy absorption is typical for elderly patients and those with dysphagia. Most often, these patients take their medication with a glass of orange juice or another liquid instead of a glass of water. We conducted a dissolution test with gastro-resistant tablets acetylsalicylic acid where different kind of orange juice or soft drink wеre added to the release medium. As a control, release medium - buffers 1.2, 4.5 and 6.8 were used. The released aspirin was determined after HPLC analysis. The obtained data were fitted to different kinetic models. The results of the dissolution test in medium buffers with added different beverage showed results similar to those obtained in pure buffer, where it is used an artificial sweetener and different, when sugar or glucose-fructose syrup was used to sweeten the beverage. The most significant change was observed in the release kinetics of the active substance.To exclude the possibility that the other beverage ingredients or excipients used to make the tablets affect the release profile of acetylsalicylic acid, we conducted a beverage-like dissolution test. Instead of a original beverage, we used water and sugar syrup, in a concentration that is declared on the label of the original beverages. The results obtained confirm that different sugar concentrations alter the release profile of acetylsalicylic acid from gastro resistant tablets when they are taken with a glass of sugar-containing beverage instead of a glass of water.