HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants

Virus Genes ◽  
2010 ◽  
Vol 40 (3) ◽  
pp. 440-446 ◽  
Author(s):  
Noriho Fukuzawa ◽  
Noriko Itchoda ◽  
Takeaki Ishihara ◽  
Kazunori Goto ◽  
Chikara Masuta ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor

scholarly journals Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Heiko Ziebell ◽  
Alex M. Murphy ◽  
Simon C. Groen ◽  
Trisna Tungadi ◽  
Jack H. Westwood ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor

scholarly journals Cucumber Mosaic Virus 2b Protein Subcellular Targets and Interactions: Their Significance to RNA Silencing Suppressor Activity

2010 ◽  
Vol 23 (3) ◽  
pp. 294-303 ◽  
Author(s):  
Inmaculada González ◽  
Llucia Martínez ◽  
Daria V. Rakitina ◽  
Mathew G. Lewsey ◽  
Félix A. Atencio ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Small Rnas ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor ◽  
2B Protein

The RNA silencing suppressor activity of the 2b protein of Cucumber mosaic virus (CMV) has been variously attributed to its nuclear targeting, its interaction with and inhibition of Argonaute 1 (AGO1), or its ability to bind small RNAs in vitro. In addition, the 2b ortholog of Tomato aspermy virus forms aggregates and binds RNAs in vitro. We have further studied the relationships between CMV 2b protein silencing suppressor activity and its subcellular distribution, protein-protein interactions in vivo, and interactions with small interfering RNAs in vitro. To do this, we tagged the protein with fluorescent markers and showed that it retained suppressor activity. We showed that the 2b protein is present in the nucleolus and that it self-interacts and interacts with AGO1 and AGO4 in vivo. Using a battery of mutants, we showed that the putative nuclear localization signals and phosphorylation motif of the 2b protein are not required for self-interaction or for interaction with AGO proteins. The occurrence of neither of these interactions or of nucleolar targeting was sufficient to provide local silencing-suppression activity. In contrast, the ability of the 2b protein to bind small RNAs appears to be indispensable for silencing suppressor function.

A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector

2019 ◽  
Vol 272 ◽  
pp. 197733 ◽  
Author(s):  
Mireya Martínez-Pérez ◽  
José A. Navarro ◽  
Vicente Pallás ◽  
Jesús A. Sánchez-Navarro
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Expression Vector ◽  
Alfalfa Mosaic Virus ◽  
Silencing Suppressor ◽  
Activity Assay ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor ◽  
Virus Expression

scholarly journals A cucumber mosaic virus mutant lacking the 2b counter-defence protein gene provides protection against wild-type strains

2007 ◽  
Vol 88 (10) ◽  
pp. 2862-2871 ◽  
Author(s):  
Heiko Ziebell ◽  
Tina Payne ◽  
James O. Berry ◽  
John A. Walsh ◽  
John P. Carr
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Cross Protection ◽  
Host Cells ◽  
Silencing Suppressor ◽  
Wild Type ◽  
Rna Sequences ◽  
Challenge Inoculation ◽  
Virus Mutant

Several plant virus mutants, in which genes encoding silencing suppressor proteins have been deleted, are known to induce systemic or localized RNA silencing against themselves and other RNA molecules containing homologous sequences. Thus, it is thought that many cases of cross-protection, in which infection with a mild or asymptomatic virus mutant protects plants against challenge infection with closely related virulent viruses, can be explained by RNA silencing. We found that a cucumber mosaic virus (CMV) mutant of the subgroup IA strain Fny (Fny-CMVΔ2b), which cannot express the 2b silencing suppressor protein, cross-protects tobacco (Nicotiana tabacum) and Nicotiana benthamiana plants against disease induction by wild-type Fny-CMV. However, protection is most effective only if inoculation with Fny-CMVΔ2b and challenge inoculation with wild-type CMV occurs on the same leaf. Unexpectedly, Fny-CMVΔ2b also protected plants against infection with TC-CMV, a subgroup II strain that is not closely related to Fny-CMV. Additionally, in situ hybridization revealed that Fny-CMVΔ2b and Fny-CMV can co-exist in the same tissues but these tissues contain zones of Fny-CMVΔ2b-infected host cells from which Fny-CMV appears to be excluded. Taken together, it appears unlikely that cross-protection by Fny-CMVΔ2b occurs by induction of systemic RNA silencing against itself and homologous RNA sequences in wild-type CMV. It is more likely that protection occurs through either induction of very highly localized RNA silencing, or by competition between strains for host cells or resources.

The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor

2013 ◽  
Vol 177 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Liying Sun ◽  
Ida Bagus Andika ◽  
Jiangfeng Shen ◽  
Di Yang ◽  
Claudio Ratti ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor ◽  
Chinese Wheat

RNA Silencing Suppressor p19 Regulates The Expressions of Cell Cycle Related Genes*

2009 ◽  
Vol 36 (5) ◽  
pp. 541-548 ◽  
Author(s):  
Li LIU ◽  
Jian LI ◽  
Yu-Ping XU ◽  
Wen-Tao QIAO ◽  
Qi-Min CHEN ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor
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