scholarly journals Identification of the RNA silencing suppressor activity of sugarcane streak mosaic virus P1 gene

VirusDisease ◽  
2020 ◽  
Vol 31 (3) ◽  
pp. 333-340
Author(s):  
Krishnamoorthy Bagyalakshmi ◽  
Rasappa Viswanathan
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Sugarcane Streak Mosaic Virus ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor

A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector

2019 ◽  
Vol 272 ◽  
pp. 197733 ◽  
Author(s):  
Mireya Martínez-Pérez ◽  
José A. Navarro ◽  
Vicente Pallás ◽  
Jesús A. Sánchez-Navarro
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Expression Vector ◽  
Alfalfa Mosaic Virus ◽  
Silencing Suppressor ◽  
Activity Assay ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor ◽  
Virus Expression

scholarly journals Cucumber Mosaic Virus 2b Protein Subcellular Targets and Interactions: Their Significance to RNA Silencing Suppressor Activity

2010 ◽  
Vol 23 (3) ◽  
pp. 294-303 ◽  
Author(s):  
Inmaculada González ◽  
Llucia Martínez ◽  
Daria V. Rakitina ◽  
Mathew G. Lewsey ◽  
Félix A. Atencio ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Small Rnas ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Suppressor Activity ◽  
Rna Silencing Suppressor ◽  
2B Protein

The RNA silencing suppressor activity of the 2b protein of Cucumber mosaic virus (CMV) has been variously attributed to its nuclear targeting, its interaction with and inhibition of Argonaute 1 (AGO1), or its ability to bind small RNAs in vitro. In addition, the 2b ortholog of Tomato aspermy virus forms aggregates and binds RNAs in vitro. We have further studied the relationships between CMV 2b protein silencing suppressor activity and its subcellular distribution, protein-protein interactions in vivo, and interactions with small interfering RNAs in vitro. To do this, we tagged the protein with fluorescent markers and showed that it retained suppressor activity. We showed that the 2b protein is present in the nucleolus and that it self-interacts and interacts with AGO1 and AGO4 in vivo. Using a battery of mutants, we showed that the putative nuclear localization signals and phosphorylation motif of the 2b protein are not required for self-interaction or for interaction with AGO proteins. The occurrence of neither of these interactions or of nucleolar targeting was sufficient to provide local silencing-suppression activity. In contrast, the ability of the 2b protein to bind small RNAs appears to be indispensable for silencing suppressor function.

scholarly journals A critical domain of theCucumber mosaic virus2b protein for RNA silencing suppressor activity

FEBS Letters ◽  
2008 ◽  
Vol 583 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Jian Ye ◽  
Jing Qu ◽  
Jian-Feng Zhang ◽  
Yun-Feng Geng ◽  
Rong-Xiang Fang
Keyword(s):  
Rna Silencing ◽  
Silencing Suppressor ◽  
Suppressor Activity ◽  
Critical Domain ◽  
Rna Silencing Suppressor

scholarly journals Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Heiko Ziebell ◽  
Alex M. Murphy ◽  
Simon C. Groen ◽  
Trisna Tungadi ◽  
Jack H. Westwood ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor

HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants

Virus Genes ◽  
2010 ◽  
Vol 40 (3) ◽  
pp. 440-446 ◽  
Author(s):  
Noriho Fukuzawa ◽  
Noriko Itchoda ◽  
Takeaki Ishihara ◽  
Kazunori Goto ◽  
Chikara Masuta ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Silencing Suppressor ◽  
Rna Silencing Suppressor
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