Development of a Highly Sensitive Colorimetric Method for Detecting 17β-Estradiol Based on Combination of Gold Nanoparticles and Shortening DNA Aptamers

2019 ◽  
Vol 230 (6) ◽  
Author(s):  
Hongbin Pu ◽  
Zhibin Huang ◽  
Da-Wen Sun ◽  
Xiaohui Xie ◽  
Weibiao Zhou
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Dna Aptamers ◽  
Highly Sensitive ◽  
17Β Estradiol

scholarly journals Highly sensitive colorimetric detection of 17β-estradiol using split DNA aptamers immobilized on unmodified gold nanoparticles

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Jinchuan Liu ◽  
Wenhui Bai ◽  
Shucao Niu ◽  
Chao Zhu ◽  
Shuming Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Dna Aptamers ◽  
Highly Sensitive ◽  
17Β Estradiol

scholarly journals Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2679 ◽  
Author(s):  
Zheng-Jun Xie ◽  
Xian-Yu Bao ◽  
Chi-Fang Peng
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Limit Of Detection ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Optimal Conditions ◽  
Surface Deposition ◽  
Highly Sensitive ◽  
Dna Strand

A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+ by centrifugation. It was found that the CH3Hg+ reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2 was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+ with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+ detection in environmental samples.

scholarly journals A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2774-2778
Author(s):  
Jiahui Wen ◽  
Yongming Liu ◽  
Jingwen Li ◽  
Hao Lin ◽  
Yiran Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Nuclease Activity ◽  
Colorimetric Sensor ◽  
Sensitive Detection ◽  
Label Free ◽  
S1 Nuclease ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A label-free, simple and rapid colorimetric method for the sensitive detection of S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles.

scholarly journals Highly sensitive detection of estradiol by a SERS sensor based on TiO2 covered with gold nanoparticles

2020 ◽  
Vol 11 ◽  
pp. 1026-1035 ◽  
Author(s):  
Andrea Brognara ◽  
Ili F Mohamad Ali Nasri ◽  
Beatrice R Bricchi ◽  
Andrea Li Bassi ◽  
Caroline Gauchotte-Lindsay ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Gold Deposition ◽  
Annealing Conditions ◽  
Highly Sensitive ◽  
Surface Enhanced Raman ◽  
17Β Estradiol ◽  
Enhanced Raman Scattering ◽  
Highly Sensitive Detection

We propose the use of gold nanoparticles grown on the surface of nanoporous TiO2 films as surface-enhanced Raman scattering (SERS) sensors for the detection of 17β-estradiol. Gold deposition on top of a TiO2 surface leads to the formation of nanoparticles the plasmonic properties of which fulfil the requirements of a SERS sensor. The morphological and optical properties of the surface were investigated. Specifically, we demonstrate that the TiO2 background pressure during pulsed laser deposition and the annealing conditions offer control over the formation of Au nanoparticles with different sizes, shapes and distributions, yielding a versatile sensor. We have exploited the surface for the detection of 17β-estradiol, an emerging contaminant in environmental waters. We have found a limit of detection of 1 nM with a sensitivity allowing for a dynamic range of five orders of magnitude (up to 100 µM).

scholarly journals Highly sensitive detection of estradiol by a SERS sensor based on TiO2 covered with gold nanoparticles

2020 ◽  
Author(s):  
Andrea Brognara ◽  
Ili F Mohamad Ali Nasri ◽  
Beatrice R Bricchi ◽  
Andrea Li Bassi ◽  
Caroline Gauchotte-Lindsay ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Gold Deposition ◽  
Annealing Conditions ◽  
Highly Sensitive ◽  
Surface Enhanced Raman ◽  
17Β Estradiol ◽  
Enhanced Raman Scattering ◽  
Highly Sensitive Detection

We propose the use of gold nanoparticles grown on the surface of nanoporous TiO2 films as Surface Enhanced Raman Scattering (SERS) sensors for the detection of 17β-estradiol. Gold deposition on top of TiO2 surfaces leads to the formation of nanoparticles, which plasmonic properties fit the requirements of a SERS sensor well. The morphological and optical properties of this surface were investigated. Specifically, we demonstrated that the TiO2 background pressure during pulsed laser deposition and annealing conditions enabled the formation of a variety of Au nanoparticles with controlled size, shape and distribution thus resulting in a versatile sensor. We have exploited this surface for the detection of 17β-estradiol, an emerging contaminant in environmental waters. We have found a limit of detection of 10 nM with a sensitivity allowing dynamic range of five orders of magnitude (up to 100 µM).

Synthesis and application of polycation-stabilized gold nanoparticles as a highly sensitive sensor for molecular cysteine determination

2021 ◽  
pp. 106481
Author(s):  
Quang Khanh Nguyen ◽  
Thi Hieu Hoang ◽  
Xuan Thanh Bui ◽  
Thi Anh Huong Nguyen ◽  
Tien Duc Pham ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Highly Sensitive ◽  
Sensitive Sensor

scholarly journals A dual colorimetric probe for rapid environmental monitoring of Hg2+ and As3+ using gold nanoparticles functionalized with d-penicillamine

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5456-5465
Author(s):  
Su-Jin Yoon ◽  
Yun-Sik Nam ◽  
Yeonhee Lee ◽  
In Hwan Oh ◽  
Kang-Bong Lee
Keyword(s):  
Gold Nanoparticles ◽  
Environmental Monitoring ◽  
Colorimetric Assay ◽  
Colorimetric Probe ◽  
Highly Sensitive ◽  
Dual Detection

A highly sensitive and selective colorimetric assay for the dual detection of Hg2+ and As3+ using gold nanoparticles (AuNPs) conjugated with d-penicillamine (DPL) was developed.

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

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