scholarly journals Development of a Three-Dimensional In Vitro Model for Longitudinal Observation of Cell Behavior: Monitoring by Magnetic Resonance Imaging and Optical Imaging

2009 ◽  
Vol 12 (4) ◽  
pp. 367-376 ◽  
Author(s):  
Klaus Kruttwig ◽  
Chantal Brueggemann ◽  
Eric Kaijzel ◽  
Susanne Vorhagen ◽  
Thomas Hilger ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Cell Behavior ◽  
Resonance Imaging ◽  
Behavior Monitoring ◽  
Longitudinal Observation ◽  
Vitro Model

scholarly journals Transthoracic three-dimensional echocardiographic reconstruction of left and right ventricles: In vitro validation and comparison with magnetic resonance imaging

1997 ◽  
Vol 133 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Riccardo Pini ◽  
Giuseppe Giannazzo ◽  
Mauro Di Bari ◽  
Francesca Innocenti ◽  
Luigi Rega ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Three Dimensional ◽  
Resonance Imaging ◽  
Left And Right

scholarly journals Label-free magnetic resonance imaging to locate live cells in three-dimensional porous scaffolds

2012 ◽  
Vol 9 (74) ◽  
pp. 2321-2331 ◽  
Author(s):  
A. Abarrategi ◽  
M. E. Fernandez-Valle ◽  
T. Desmet ◽  
D. Castejón ◽  
A. Civantos ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Live Cells ◽  
Porous Scaffolds ◽  
Label Free ◽  
Resonance Imaging ◽  
Critical Feature

Porous scaffolds are widely tested materials used for various purposes in tissue engineering. A critical feature of a porous scaffold is its ability to allow cell migration and growth on its inner surface. Up to now, there has not been a method to locate live cells deep inside a material, or in an entire structure, using real-time imaging and a non-destructive technique. Herein, we seek to demonstrate the feasibility of the magnetic resonance imaging (MRI) technique as a method to detect and locate in vitro non-labelled live cells in an entire porous material. Our results show that the use of optimized MRI parameters (4.7 T; repetition time = 3000 ms; echo time = 20 ms; resolution 39 × 39 µm) makes it possible to obtain images of the scaffold structure and to locate live non-labelled cells in the entire material, with a signal intensity higher than that obtained in the culture medium. In the current study, cells are visualized and located in different kinds of porous scaffolds. Moreover, further development of this MRI method might be useful in several three-dimensional biomaterial tests such as cell distribution studies, routine qualitative testing methods and in situ monitoring of cells inside scaffolds.

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