Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer

2016 ◽  
Vol 24 (4) ◽  
pp. 3748-3758 ◽  
Author(s):  
Petr Masner ◽  
Barbora Javůrková ◽  
Luděk Bláha
2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Anne Klöppel ◽  
Franz Brümmer ◽  
Denise Schwabe ◽  
Gertrud Morlock

The sessile suspension-feeding wormsnailDendropoma maxima, Sowerby 1825 (Vermetidae) secretes a mucus net to capture planktonic prey. The nets are spread out over the corals and often have remarkable deleterious effects on them like changes in growth form and pigmentation shifts not uncommonly resulting in tissue necrosis. Until now, there is no explanation for this phenomenon although the indication as well as theories about its genesis is mentioned in several publications. Vermetids are well studied concerning the intraspecific competition with neighboring individuals but not in their interaction with other taxa like corals or fish. We did extensivein situvideo recording and observed that fish avoided the plankton-load nets although several specialized taxa are known to be molluscivores, mucivores, and/or feed on plankton. As many molluscs use chemical weapons to combat feeding pressure and to defend themselves against predators, we screened empty and plankton-load mucus nets for potential bioactive metabolites. Bioactivity testing was performed with a recently developed system based on a chromatographic separation (high-performance thin-layer chromatography (HPTLC)) and a bioassay with luminescent bacteriaVibrio fischeri. Thus, we found at least two active compounds exclusively accumulated by the wormsnails themselves. This is the first record of bioactive properties in the whole family of Vermetidae.


Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1349
Author(s):  
Uwe Strotmann ◽  
Daniel Pastor Flores ◽  
Odorico Konrad ◽  
Cornelia Gendig

The activated sludge respiration inhibition test and the luminescent bacteria test with Vibrio fischeri are important bacterial test systems for evaluation of the toxicity of chemical compounds. These test systems were further optimized to result in better handling, reliability and sensitivity. Concerning the Vibrio fischeri test, media components such as yeast extract and bivalent cation concentrations like Ca2+ and Mg2+ were optimized. The cultivation, storage conditions and reactivation process of the stored bacteria were also improved, which enabled simpler handling and led to good reproducibility. Additionally, the respiration inhibition test with a prolonged incubation time was further analyzed using different chlorinated phenols as reference compounds. It could be stated that a longer incubation period significantly improved the sensitivity of the test system.


Author(s):  
M.D. Norman ◽  
C.C. Lu

Recent attention to members of the sepiolid squid genusEuprymnaand symbiotic associations with luminescent bacteria (Vibrio fischeristrains) has prompted a review of this poorly-resolved group of squids. Twelve nominal species have been placed in this genus of which the majority are ill-defined, known only from their original descriptions and separated on the basis of inadequate characters. As a first step in resolving this group, a temperate Australian species, the Southern dumpling squid,Euprymna tasmanica, is here redescribed in detail. As the genusEuprymnacurrently stands, most members are only distinguished on the number and position of enlarged suckers in mature males. No diagnostic characters are available to identify females. All nominal species placed in this genus are reviewed and a key to proposed valid species is presented. Six species are considered here to be valid:Euprymna berryi, E. hoylei, E. morsei, E. scolopes, E. tasmanicaand an undescribed species treated here asEuprymnasp. 1.Euprymna similisis a synonym ofE. morseiof Japan. Due to inadequate original descriptions, and lost or poor type material, two species are considered here to benomen dubia(E. schneehageniandE. pusilla), while the taxonomic status of four additional species remain unresolved (E. albatrossae, E. bursa, E. phenaxandE. stenodactyla).


2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Aziz Babapoor ◽  
Reza Hajimohammadi ◽  
Seyyed Mohammad Jokar ◽  
Meysam Paar

In this study, a biosensor is designed to remove mercury as a toxic metal contaminant from the soil. The rhamnolipid biosurfactant was used to extract the mercury sorbed to soil to the aqueous phase. An immobilized bioluminescent bacterium (Escherichia coli MC106) with pmerRBPmerlux plasmid is assisted for mercury detection. A significant decrease in luminescence level was observed in a biosensor system containing contaminated soil sample extract. The concentrations of extracting mercury are well correlated with the mercury toxicity data obtained from experimental biosensor systems according to the RBL value. The optimum aeration rate of 20 ml/min was obtained for the biosensor systems. The advantage of such a biosensor is the in situ quantification of mercury as a heavy metal contaminant in soils. Therefore, this system could be proposed as a good biosensor-based alternative for future detection of heavy toxic metals in soils.


Chemosphere ◽  
1997 ◽  
Vol 35 (12) ◽  
pp. 2925-2938 ◽  
Author(s):  
T. Backhaus ◽  
K. Froehner ◽  
R. Altenburger ◽  
L.H. Grimme

2001 ◽  
Vol 2001 (12) ◽  
pp. 714-719
Author(s):  
W.J. Rue ◽  
R. Connelly ◽  
K. Dixon ◽  
W. McCulloch ◽  
L. Christensen ◽  
...  

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