Thermo-alkali-stable α-carbonic anhydrase of Bacillus halodurans: heterologous expression in Pichia pastoris and applicability in carbon sequestration

2017 ◽  
Vol 25 (7) ◽  
pp. 6838-6849 ◽  
Author(s):  
Shazia Faridi ◽  
Tulasi Satyanarayana
Keyword(s):  
Carbon Sequestration ◽  
Pichia Pastoris ◽  
Carbonic Anhydrase ◽  
Heterologous Expression ◽  
Bacillus Halodurans

Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris

2013 ◽  
Vol 54 (S1) ◽  
pp. S134-S141 ◽  
Author(s):  
Lin-Feng You ◽  
Zhi-Ming Liu ◽  
Jun-Fang Lin ◽  
Li-Qiong Guo ◽  
Xun-Liu Huang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Molecular Cloning ◽  
Ganoderma Lucidum ◽  
Laccase Gene

scholarly journals Heterologous expression in Pichia pastoris and biochemical characterization of the unmodified sulfatase from Fusarium proliferatum LE1

2017 ◽  
Vol 30 (8) ◽  
pp. 571-571 ◽  
Author(s):  
Svetlana A Korban ◽  
Kirill S Bobrov ◽  
Maria A Maynskova ◽  
Stanislav N Naryzhny ◽  
Olga L Vlasova ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Biochemical Characterization ◽  
Fusarium Proliferatum

Heterologous expression of codon optimized Trichoderma reesei Cel6A in Pichia pastoris

2016 ◽  
Vol 92 ◽  
pp. 107-116 ◽  
Author(s):  
Fubao Fuelbiol Sun ◽  
Renhui Bai ◽  
Huimin Yang ◽  
Fei Wang ◽  
Jing He ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Trichoderma Reesei

scholarly journals Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana

2018 ◽  
Vol 28 (12) ◽  
pp. 2057-2063 ◽  
Author(s):  
Wang Bo ◽  
Ying Yan ◽  
Jing Xu ◽  
Xiaoyan Fu ◽  
Hongjuan Han ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Laccaria Bicolor

scholarly journals Detection of urease and carbonic anhydrase activity using a rapid and economical field test to assess microbially-induced carbonate precipitation

2020 ◽  
Author(s):  
Fernando Medina Ferrer ◽  
Kathryn Hobart ◽  
Jake V. Bailey
Keyword(s):  
Carbon Sequestration ◽  
Carbonic Anhydrase ◽  
Marker Gene ◽  
Cost Effective ◽  
Carbonic Anhydrase Activity ◽  
Soil Restoration ◽  
Reaction Products ◽  
Calcareous Fens ◽  
Molecular Approaches

ABSTRACTMicrobial precipitation of calcium carbonate has diverse engineering applications, from building and soil restoration, to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate minerals, yet many microbial community studies rely on marker gene or metagenomic approaches that are unable to determine in situ activity. Here, we developed fast and cost-effective tests for the field detection of urease and CA activity using pH-sensitive strips inside microcentrifuge tubes that change color in response to the reaction products of urease (NH3) and CA (CO2). Samples from a saline lake, a series of calcareous fens, and ferrous springs were assayed in the field, finding relatively high urease activity in lake samples, whereas CA activity was only detected in a ferrous spring. Incubations of lake microbes with urea resulted in significantly higher CaCO3 precipitation compared to incubations with a urease inhibitor. Therefore, the rapid assay indicated an on-site active metabolism potentially mediating carbonate mineralization. Field urease and CA activity assays complement molecular approaches and facilitate the search for carbonate-precipitating microbes and their in situ activity, which could be applied toward agriculture, engineering and carbon sequestration technologies.

Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system

2018 ◽  
Vol 102 (7) ◽  
pp. 3145-3158 ◽  
Author(s):  
Fernanda Gobbi Amorim ◽  
Johara Boldrini-França ◽  
Karla de Castro Figueiredo Bordon ◽  
Iara Aimê Cardoso ◽  
Edwin De Pauw ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Scorpion Venom
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