Utility of Immobilized Recombinant Carbonic Anhydrase of Bacillus halodurans TSLV1 on the Surface of Modified Iron Magnetic Nanoparticles in Carbon Sequestration

2017 ◽  
Vol 31 (3) ◽  
pp. 3002-3009 ◽  
Author(s):  
Shazia Faridi ◽  
Himadri Bose ◽  
T. Satyanarayana
Keyword(s):  
Magnetic Nanoparticles ◽  
Carbon Sequestration ◽  
Carbonic Anhydrase ◽  
Bacillus Halodurans

scholarly journals Detection of urease and carbonic anhydrase activity using a rapid and economical field test to assess microbially-induced carbonate precipitation

2020 ◽  
Author(s):  
Fernando Medina Ferrer ◽  
Kathryn Hobart ◽  
Jake V. Bailey
Keyword(s):  
Carbon Sequestration ◽  
Carbonic Anhydrase ◽  
Marker Gene ◽  
Cost Effective ◽  
Carbonic Anhydrase Activity ◽  
Soil Restoration ◽  
Reaction Products ◽  
Calcareous Fens ◽  
Molecular Approaches

ABSTRACTMicrobial precipitation of calcium carbonate has diverse engineering applications, from building and soil restoration, to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate minerals, yet many microbial community studies rely on marker gene or metagenomic approaches that are unable to determine in situ activity. Here, we developed fast and cost-effective tests for the field detection of urease and CA activity using pH-sensitive strips inside microcentrifuge tubes that change color in response to the reaction products of urease (NH3) and CA (CO2). Samples from a saline lake, a series of calcareous fens, and ferrous springs were assayed in the field, finding relatively high urease activity in lake samples, whereas CA activity was only detected in a ferrous spring. Incubations of lake microbes with urea resulted in significantly higher CaCO3 precipitation compared to incubations with a urease inhibitor. Therefore, the rapid assay indicated an on-site active metabolism potentially mediating carbonate mineralization. Field urease and CA activity assays complement molecular approaches and facilitate the search for carbonate-precipitating microbes and their in situ activity, which could be applied toward agriculture, engineering and carbon sequestration technologies.

scholarly journals Production and covalent immobilisation of the recombinant bacterial carbonic anhydrase (SspCA) onto magnetic nanoparticles

2017 ◽  
Vol 32 (1) ◽  
pp. 759-766 ◽  
Author(s):  
Rosa Perfetto ◽  
Sonia Del Prete ◽  
Daniela Vullo ◽  
Giovanni Sansone ◽  
Carmela M.A. Barone ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Carbonic Anhydrase ◽  
Covalent Immobilisation

scholarly journals Enzymes for carbon sequestration: neutron crystallographic studies of carbonic anhydrase

2010 ◽  
Vol 66 (11) ◽  
pp. 1178-1183 ◽  
Author(s):  
S. Z. Fisher ◽  
A. Y. Kovalevsky ◽  
J. Domsic ◽  
M. Mustyakimov ◽  
D. N. Silverman ◽  
...  
Keyword(s):  
Carbon Sequestration ◽  
Carbonic Anhydrase

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO2Sequestration

2012 ◽  
Vol 18 (38) ◽  
pp. 12028-12034 ◽  
Author(s):  
Mari Vinoba ◽  
Margandan Bhagiyalakshmi ◽  
Soon Kwan Jeong ◽  
Sung Chan Nam ◽  
Yeoil Yoon
Keyword(s):  
Magnetic Nanoparticles ◽  
Carbonic Anhydrase
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