Bioaccumulation and toxicity effects of flubendiamide in zebrafish (Danio rerio)

Excessive production of melanin implicates hyperpigmentation disorders. Flavokawain A (FLA) and flavokawain B (FLB) have been reported with anti-melanogenic activity, but their melanogenic inhibition and toxicity effects on the vertebrate model of zebrafish are still unknown. In the present study, cytotoxic as well as melanogenic effects of FLA and FLB on cellular melanin content and tyrosinase activity were evaluated in α-MSH-induced B16/F10 cells. Master regulator of microphthalmia-associated transcription factor (Mitf) and the other downstream melanogenic-related genes were verified via quantitative real time PCR (qPCR). Toxicity assessment and melanogenesis inhibition on zebrafish model was further observed. FLA and FLB significantly reduced the specific cellular melanin content by 4.3-fold and 9.6-fold decrement, respectively in α-MSH-induced B16/F10 cells. Concomitantly, FLA significantly reduced the specific cellular tyrosinase activity by 7-fold whilst FLB by 9-fold. The decrement of melanin production and tyrosinase activity were correlated with the mRNA suppression of Mitf which in turn down-regulate Tyr, Trp-1 and Trp-2. FLA and FLB exhibited non-toxic effects on the zebrafish model at 25 and 6.25 µM, respectively. Further experiments on the zebrafish model demonstrated successful phenotype-based depigmenting activity of FLA and FLB under induced melanogenesis. To sum up, our findings provide an important first key step for both of the chalcone derivatives to be further studied and developed as potent depigmenting agents.

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Toxicity effects of captan on different life stages of zebrafish (Danio rerio)

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2019.04.003 ◽

2019 ◽

Vol 69 ◽

pp. 80-85 ◽

Cited By ~ 7

Author(s):

Yimeng Zhou ◽

Xiangguang Chen ◽

Miaomiao Teng ◽

Jie Zhang ◽

Chengju Wang

Keyword(s):

Danio Rerio ◽

Life Stages ◽

Toxicity Effects

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Bioaccumulation and Toxicity Effects of Flubendiamide in Zebrafish (Danio Rerio)

10.21203/rs.3.rs-862021/v1 ◽

2021 ◽

Author(s):

Zhiyuan Meng ◽

Zhichao Wang ◽

xiaojun Chen ◽

Yueyi Song ◽

Miaomiao Teng ◽

...

Keyword(s):

Danio Rerio ◽

Structural Damage ◽

Biochemical Analysis ◽

Early Stage ◽

Aquatic Organisms ◽

Histopathological Analysis ◽

Caspase 9 ◽

Somatic Index ◽

Physiological And Biochemical ◽

Toxicity Effects

Abstract As a diamide insecticide, flubendiamide is widely used and has many adverse effects on environmental organisms. In this study, bioaccumulation and toxicity effects of flubendiamide in zebrafish (Danio rerio) were studied. Specifically, the results showed that the concentrations of flubendiamide increased in the early stage and achieved steady stages at 14 days and the bioconcentration factors (BCFs) of flubendiamide in zebrafish were 1.125 - 2.011. Furthermore, flubendiamide had no significant effects on the growth phenotypes of zebrafish. However, zebrafish hepatic somatic index (HSI) of zebrafish had changed significantly with exposure. Histopathological analysis showed that exposure to flubendiamide could cause structural damage to liver tissue of zebrafish. Further physiological and biochemical analysis showed that flubendiamide could significantly change the activity of CAT and the contents of MDA and GSH in liver of zebrafish. In particular, exposure to flubendiamide could also cause significant changes in the mRNA expression levels of cell apoptosis-related genes involving p53, puma, caspase-3, caspase-9, apaf-1 and bax in liver of zebrafish. In general, these results indicated that exposure to flubendiamide could induce liver damage by inducing oxidative stress and apoptosis in liver of zebrafish. The results of this study will help to further comprehensively evaluate the safety of flubendiamide to aquatic organisms.

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