Bioaccumulation and Toxicity Effects of Flubendiamide in Zebrafish (Danio Rerio)

Abstract As a diamide insecticide, flubendiamide is widely used and has many adverse effects on environmental organisms. In this study, bioaccumulation and toxicity effects of flubendiamide in zebrafish (Danio rerio) were studied. Specifically, the results showed that the concentrations of flubendiamide increased in the early stage and achieved steady stages at 14 days and the bioconcentration factors (BCFs) of flubendiamide in zebrafish were 1.125 - 2.011. Furthermore, flubendiamide had no significant effects on the growth phenotypes of zebrafish. However, zebrafish hepatic somatic index (HSI) of zebrafish had changed significantly with exposure. Histopathological analysis showed that exposure to flubendiamide could cause structural damage to liver tissue of zebrafish. Further physiological and biochemical analysis showed that flubendiamide could significantly change the activity of CAT and the contents of MDA and GSH in liver of zebrafish. In particular, exposure to flubendiamide could also cause significant changes in the mRNA expression levels of cell apoptosis-related genes involving p53, puma, caspase-3, caspase-9, apaf-1 and bax in liver of zebrafish. In general, these results indicated that exposure to flubendiamide could induce liver damage by inducing oxidative stress and apoptosis in liver of zebrafish. The results of this study will help to further comprehensively evaluate the safety of flubendiamide to aquatic organisms.

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Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio

Aquatic Toxicology ◽

10.1016/j.aquatox.2021.105867 ◽

2021 ◽

pp. 105867

Author(s):

Ivana Milenković ◽

Ksenija Radotić ◽

Jovana Despotović ◽

Branka Lončarević ◽

Marija Lješević ◽

...

Keyword(s):

Daphnia Magna ◽

Danio Rerio ◽

Vibrio Fischeri ◽

Aquatic Organisms ◽

Ceo2 Nanoparticles

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Evaluation of leaf physiological and biochemical traits during senescence of the wheat core collection in the southwest of China

Canadian Journal of Plant Science ◽

10.4141/cjps06018 ◽

2008 ◽

Vol 88 (2) ◽

pp. 331-337 ◽

Cited By ~ 2

Author(s):

Kai Shu ◽

Pei G Luo ◽

Huai Y Zhang ◽

Ben J Yan ◽

Jie Zhang ◽

...

Keyword(s):

Core Collection ◽

Biochemical Analysis ◽

Southwest China ◽

Heading Date ◽

Leaf Traits ◽

Triticum Aestivum L ◽

Malondialdehyde Content ◽

Good Potential ◽

Chinese Wheat ◽

Physiological And Biochemical

Agronomical, physiological, and biochemical analysis of the Chinese wheat (Triticum aestivum L.) core collection revealed abundant variation in the indices examined, both within and between ecotype regions. The genotypes from the Yellow-Huai Rivers (YH) region, the Middle and lower reaches of the Yangtze River (MY) region and the Southwest China (SWC) region showed good adaptation as measured by heading date, malondialdehyde content (MDA), and superoxide dismutase (SOD) and catalase (CAT) activities and would have good potential for wheat improvement in SWC. Key words: Adaptability, core collection, diversity, leaf traits, southwest China, wheat

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Lethal effects of abamectin on the aquatic organisms Daphnia similis, Chironomus xanthus and Danio rerio

Chemosphere ◽

10.1016/j.chemosphere.2011.08.047 ◽

2012 ◽

Vol 86 (1) ◽

pp. 36-40 ◽

Cited By ~ 64

Author(s):

Andréa Novelli ◽

Bruna Horvath Vieira ◽

Daniela Cordeiro ◽

Luciana Teresa Dias Cappelini ◽

Eny Maria Vieira ◽

...

Keyword(s):

Danio Rerio ◽

Aquatic Organisms ◽

Lethal Effects ◽

Daphnia Similis

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Physiological and Biochemical Analysis of Somaclones Derived from Leaf Explants of Lathyrus sativus

Journal of Plant Biochemistry and Biotechnology ◽

10.1007/bf03262959 ◽

1995 ◽

Vol 4 (2) ◽

pp. 85-89 ◽

Cited By ~ 5

Author(s):

Nirupama Tiwari ◽

K. C. Bansal ◽

K. All ◽

Archana Sachdev ◽

I. M. Santha ◽

...

Keyword(s):

Biochemical Analysis ◽

Leaf Explants ◽

Lathyrus Sativus ◽

Physiological And Biochemical

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Genotoxic and Histopathological Effects of Chlorpyrifos on African Catfish, C. gariepinus and Ameliorative Potentials of Ackee Apple, blighia sapida Seed

Science Journal of University of Zakho ◽

10.25271/2018.6.1.422 ◽

2018 ◽

Vol 6 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Oluyinka Iyiolai ◽

A. A. Adeagbo ◽

W. S. Weliange ◽

S. I. Abdulkareem ◽

Anifowoshe T. Abass ◽

...

Keyword(s):

Chronic Exposure ◽

Clarias Gariepinus ◽

Aquatic Organisms ◽

Acute Exposure ◽

Histopathological Analysis ◽

African Catfish ◽

Dependent Manner ◽

Dose Dependent ◽

Histopathological Effects ◽

Positive Controls

Chlorpyrifos (CPF) is a very toxic pesticide commonly used for controlling agriculturally important pests. The present study investigates DNA damaging effects of CPF on Clarias gariepinus was assessed using genetic assays. Fish were exposed to varying concentrations of CPF (100ppm, 200ppm and 300ppm) at 96 hours (acute exposure). LC50 of the pesticide was found to be 120ppm and one-tenth of the LC50 (12ppm) was taken for the chronic exposure. Distilled water and colchicine were used as negative and positive controls respectively. After 28 days of chronic exposure, fish were fed with inclusion of B. sapida seed (powder) for 14 days. Liver and gills of the fish were removed following 96h exposure, days 7, 14 and 28 of the chronic exposure as well as after the period of amelioration (14 days) for CA assay and histopathological analysis. The results of CA assay showed statistically significant (p ˂ 0.05) increase in CA in a dose-dependent manner for all the exposed groups after acute exposure and time dependent after chronic exposure. Also, this study showed that CPF can potentially induce genotoxic and histopathological changes in fish and other aquatic organisms.

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Urachal adenocarcinoma - case report and literature review

Vojnosanitetski pregled ◽

10.2298/vsp160827009p ◽

2018 ◽

Vol 75 (10) ◽

pp. 1045-1048

Author(s):

Milan Petrovic ◽

Vladimir Vasic ◽

Ljubinka Jankovic-Velickovic ◽

Srdjan Sterovic ◽

Tomislav Pejcic ◽

...

Keyword(s):

Case Report ◽

Early Stage ◽

Histopathological Analysis ◽

Complete Excision ◽

Urachal Carcinoma ◽

Term Survival ◽

Histologic Subtype ◽

Mucin Production ◽

Common Histologic Subtype ◽

Urachal Adenocarcinoma

Introduction. Urachal adenocarcinoma is extremely rare and comprises from 0.35% to 0.7% of all bladder tumors. The most common histologic subtype of urachal tumors is adenocarcinoma which can be associated with intestinal metaplasia and mucin production. Case report. We report a case of a 53-year-old patient who attented a urologist because of an intermittent haematuria lasting for three months. The ultrasound examination detected infiltration of the bladder at the fundus, 24 ? 29 mm in diameter. By the same wall, next to the tumor, there was an oval hypoechoic lesion about 40 mm in diameter. Computed tomography scan showed a solid, echogenic, strictly limited tumor at the fundus of the bladder, anteriorly, 32 ? 35 ? 22 mm in diameter which was positive after contrast application. The patient underwent partial cystectomy with complete excision of the tumor lesion 1.5 cm in healthy tissue. Histopathological analysis showed diagnosis of Adenocarcinoma mucinosum vesicae urinariae infiltrans. Patohistological findings detected a part of the urachal wall with a thin layer of fibromuscular tissue, chronic inflammation, microcalcifications in the lumen, flattened and desquamated epithelium. One year after the surgery, there were no signs of primary disease or metastases in other organs. Conclusion. Urachal adenocarcinoma is extremely rare. Long term survival could be achieved by surgical treatment in the early stage of the disease which consists of complete resection of urachal carcinoma and partial or total cystectomy.

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Zebrafish (Danio rerio) model as an early stage screening tool to study the biodistribution and toxicity profile of doxorubicin-loaded mixed micelles

Toxicology and Applied Pharmacology ◽

10.1016/j.taap.2018.07.019 ◽

2018 ◽

Vol 357 ◽

pp. 106-114 ◽

Cited By ~ 9

Author(s):

María Natalia Calienni ◽

Maximiliano Cagel ◽

Jorge Montanari ◽

Marcela A. Moretton ◽

Maria Jimena Prieto ◽

...

Keyword(s):

Danio Rerio ◽

Mixed Micelles ◽

Screening Tool ◽

Early Stage ◽

Toxicity Profile

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Acute toxicity of polychlorinated diphenyl ethers (PCDEs) in three model aquatic organisms (Scenedesmus obliquus, Daphnia magna, and Danio rerio) of different trophic levels

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.150366 ◽

2022 ◽

Vol 805 ◽

pp. 150366

Author(s):

Wenhui Yang ◽

Xinxin Huang ◽

Qiuxuan Wu ◽

Jiaqi Shi ◽

Xuesheng Zhang ◽

...

Keyword(s):

Acute Toxicity ◽

Daphnia Magna ◽

Danio Rerio ◽

Scenedesmus Obliquus ◽

Aquatic Organisms ◽

Trophic Levels ◽

Diphenyl Ethers ◽

Polychlorinated Diphenyl Ethers

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Melanogenic Inhibition and Toxicity Assessment of Flavokawain A and B on B16/F10 Melanoma Cells and Zebrafish (Danio rerio)

Molecules ◽

10.3390/molecules25153403 ◽

2020 ◽

Vol 25 (15) ◽

pp. 3403

Author(s):

Nurshafika Mohd Sakeh ◽

Nurliyana Najwa Md Razip ◽

Farah Idayu Mohd Ma’in ◽

Mohammad Nazri Abdul Bahari ◽

Naimah Latif ◽

...

Keyword(s):

Danio Rerio ◽

Toxicity Assessment ◽

Tyrosinase Activity ◽

The Other ◽

Quantitative Real Time Pcr ◽

Melanin Content ◽

Melanin Production ◽

Zebrafish Model ◽

Vertebrate Model ◽

Toxicity Effects

Excessive production of melanin implicates hyperpigmentation disorders. Flavokawain A (FLA) and flavokawain B (FLB) have been reported with anti-melanogenic activity, but their melanogenic inhibition and toxicity effects on the vertebrate model of zebrafish are still unknown. In the present study, cytotoxic as well as melanogenic effects of FLA and FLB on cellular melanin content and tyrosinase activity were evaluated in α-MSH-induced B16/F10 cells. Master regulator of microphthalmia-associated transcription factor (Mitf) and the other downstream melanogenic-related genes were verified via quantitative real time PCR (qPCR). Toxicity assessment and melanogenesis inhibition on zebrafish model was further observed. FLA and FLB significantly reduced the specific cellular melanin content by 4.3-fold and 9.6-fold decrement, respectively in α-MSH-induced B16/F10 cells. Concomitantly, FLA significantly reduced the specific cellular tyrosinase activity by 7-fold whilst FLB by 9-fold. The decrement of melanin production and tyrosinase activity were correlated with the mRNA suppression of Mitf which in turn down-regulate Tyr, Trp-1 and Trp-2. FLA and FLB exhibited non-toxic effects on the zebrafish model at 25 and 6.25 µM, respectively. Further experiments on the zebrafish model demonstrated successful phenotype-based depigmenting activity of FLA and FLB under induced melanogenesis. To sum up, our findings provide an important first key step for both of the chalcone derivatives to be further studied and developed as potent depigmenting agents.

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