Soil bacterial community structure and extracellular enzyme activities under different land use types in a long-term reclaimed wetland

2019 ◽  
Vol 19 (5) ◽  
pp. 2543-2557 ◽  
Author(s):  
Lingling Huang ◽  
Wanjin Hu ◽  
Jin Tao ◽  
Yizhen Liu ◽  
Zhaoyu Kong ◽  
...  
Keyword(s):  
Land Use ◽  
Community Structure ◽  
Bacterial Community ◽  
Enzyme Activities ◽  
Bacterial Community Structure ◽  
Extracellular Enzyme ◽  
Extracellular Enzyme Activities ◽  
Land Use Types ◽  
Soil Bacterial Community Structure

Effects of Long-Term Elevated CO2 on Rhizosphere and Bulk Soil Bacterial Community Structure in Pinus sylvestriformis Seedlings Fields

2011 ◽  
Vol 343-344 ◽  
pp. 351-356
Author(s):  
Xia Jia ◽  
Chun Juan Zhou
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Pinus Sylvestriformis ◽  
Soil Bacterial Community Structure ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain

The effect of long-term elevated CO2(as open top chambers) on rhizosphere and bulk bacterial community structure in Pinus sylvestriformis seedlings field was investigated in July, August, and September. The bacterial communities were processed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of bacterial 16S rDNA fragments amplified by PCR (Polymerase Chain Reaction) from DNA extracted directly from soil. DGGE profiles from rhizosphere samples showed large changes in rhizosphere bacterial community under elevated CO2compared to ambient except for that in September. For bulk samples, bacterial community structure changed when exposed to elevated CO2in three months. With the exception of bulk samples in August, a similitude of bacterial communities structures existed between different elevated CO2concentrations by analyzing UPGMA dendrogram based on Jaccard’s coefficient.

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