Poultry biodiversity for alternative farming systems development

Poultry biodiversity represents a key factor to improve poultry resilience and promote sustainable and low input farming systems. The EU and member states promote protection of livestock biodiversity and the development of alternative farming through funding projects such as “Local Chicken Breeds in Alternative Production Chain: Welfare, Quality and Sustainability” (funded by the Italian Ministry of Research and University). The aim of the present research was to identify among five different poultry genotypes Bionda Piemontese (BP), Robusta Maculata (RM), RM x Sasso (RMxS), BP x Sasso (BPxS) and a commercial hybrid (Ross 308) the best suitable breed in terms of productivity and welfare for alternative housing system. A total of 300 (60 x genotype), 21 days old male birds were randomly allotted in two housing systems: 1) standard intensive farming (controlled environment, 33 kg/m2 and standard diet) and 2) free-range (“natural” environmental conditions, 21 kg/m2, access to outdoor area and low-input diet). Slaughtering was performed at 81 days of age. During the trial, the productive performance and behaviour of the animals were evaluated. The housing system, the genotype and their interaction significantly affected many of the studied variables, showing broiler not the ideal genotype for extensive farming system, which is more suited for low/medium performance strains.

The impact of low input DNA on the reliability of DNA methylation as measured by the Illumina Infinium MethylationEPIC BeadChip

Background: DNA methylation (DNAm) is commonly assayed using the Illumina Infinium MethylationEPIC BeadChip, but there is currently little published evidence to define the lower limits of the amount of DNA that can be used whilst preserving data quality. Such evidence is valuable for analyses utilising precious or limited DNA sources. Materials and methods: We use a single pooled sample of DNA in quadruplicate at three dilutions to define replicability and noise, and an independent population dataset of 328 individuals (from a community-based study including US-born non-Hispanic Black and white persons) to assess the impact of total DNA input on the quality of data generated using the Illumina Infinium MethylationEPIC BeadChip. Results: Data are less reliable and more noisy as DNA input decreases to 40ng, with clear reductions in data quality; however samples with a total input as low as 40ng pass standard quality control tests, and we observe little evidence that low input DNA obscures the associations between DNAm and two phenotypes, age and smoking status. Conclusions: DNA input as low as 40ng can be used with the Illumina Infinium MethylationEPIC BeadChip, provided quality checks and sensitivity analyses are undertaken.

Do Crossbreeding using Exotic Breeds in Goat is the Right Solution for a Low-input Production System in Ethiopia? : A Review

Analysis and evaluation of the previous genetic improvement attempts and their fruition are paramount to make the right decision in the future. Hence, this paper reviews the status of goat genetic improvement programs through quantitative evidence and elucidates how it can be implemented in the future through an intensive literature review. Goat genetic improvement through crossbreeding was initiated early in 1975. However, most crossbreeding programs have lacked analysis of the existing resources and infrastructure and also lack long-term strategies. As a result, crossbreeding program was discontinued without significant contribution due to incompatibility of the exotic genotype with low-input production systems. On the other hand, the moderate to high genetic variation within a population open the window for within-breed selection. Accordingly, a well-designed within-breed selection program was initiated late in 2013 for specified breeds. Currently, governmental and non-governmental institutions plan to scale up community-based within-breed selection program. Besides, the efficiency of assisted reproductive technologies in goat genetic improvement was evaluated by ICARDA and reported a moderate achievement. However, the application of molecular technologies in Ethiopia is only limited to diversity studies. Nevertheless, there is an opportunity to use molecular technologies to enhance the genetic progress of a genetic improvement program. In conclusion, the expected benefits from crossbreeding program were not obtained and will not be obtained under the existing low input-production system. Therefore, a within-breed selection program would be an ideal option for the existing low-input production system if integrated with assisted reproductive and molecular technologies.

Effect of Different Agricultural Farming Practices on Microbial Biomass and Enzyme Activities of Celery Growing Field Soil

Soil quality is directly affected by alterations in its microbiological, biological, biochemical, physical, and chemical aspects. The microbiological activities of soil can affect soil fertility and plant growth because it can speed up the cycling of nutrients, enzymes, and hormones that are needed by plants for proper growth and development. The use of different agricultural management practices can influence microbial biomass and enzyme activities by altering soil microclimate, soil microorganism habitat, and nutrient cycling. Based on this, the present work planned to evaluate the impact of conventional, low-input, and organic farming systems in a vegetable field growing celery on microbial biomass and different soil enzyme activities. The present study showed a comparison of the effect of different practices on biological soil quality indicators during two sampling times, i.e., one month after colonization and one month before harvesting. It was observed that the soil microbial biomass in the organic farming system was significantly higher than that found in conventional and low-input practices. Under an organic farming system, the soil microbial biomass in December was significantly higher than that in October. The soil microbial biomass carbon in the 0–20 cm soil layer showed higher variation compared to that in the 20–40 cm layer for all the three of the farming management practices that were used in the study. Additionally, the soil total carbon and total organic carbon were recorded as being higher in the December samples than they were in the October samples. Under all the three of the management practices that were applied, the soil catalase activity was higher in the October samples than it was in the December soil samples that were collected the from 20–40 cm soil layer compared to those that were taken from the 0–20 cm layer. The application of organic fertilizer (chicken and cowmanure compost) resulted inincreases in the soil urease and in the protease activity. The protease activity of the soil samples that were extracted from the 0–20 cm and 20–40 cm soil layers in October was higher in the samples that were taken from farms using conventional practices than it was in the samples that were taken from farms using organic and low-input practices, while the samples that were collected during December from both of the soil layers showed higher protease activity when organic methods had been used. No significant variation in the soil urease activity was observed between the two soil layer samples. Urease activity was the highest when organic management practices were being used, followed by the low-input and the conventional modes. For the conventional and low-input practices, the soil urease activity showed an obvious trend of change that was related to thetime of sampling, i.e., activity in December was significantly higher than activity in October. The novelty of this study was to determine the microbial biomass carbon and enzymatic activity in a six-field crop rotation (tomato, cucumber, celery, fennel, cauliflower, and eggplant) using three management practices: low-input, conventional, and organic systems. The present study showed that the long-term application of organic fertilizers plays a large role in maintaining excellent microbial and enzyme activitythat result in improved soil quality.